Name: 5-Aminoallyl-dU Phosphoramidite [5-Aminoallyl-dU CEP]
Brand: AAT Bioquest
SKU: 6207
Price: 301 USD
Availability: InStock

5-Aminoallyl-dU Phosphoramidite

5-Aminoallyl-dU CEP

5-Aminoallyl-dU is a phosphoramidite building block that introduces a reactive primary amine group into oligonucleotides for post-synthetic conjugation with fluorescent dyes, enzymes, or other labels.

Versatile Conjugation Platform: Reactive primary amine enables post-synthetic coupling with NHS-ester dyes, biotin, or enzymes for microarray, FISH, and PCR applications
Superior Incorporation Efficiency: Higher labeling efficiency than direct Cy-dye incorporation due to smaller aminoallyl group versus bulky fluorophores
Standard Synthesis Compatibility: Uses normal coupling conditions and deprotection protocols without requiring specialized reagents or extended reaction times
Cost-Effective Labeling: More economical than direct Cy-modified nucleotide methods while providing better labeling consistency

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
6207	100 umoles	Price

Overview

5-Aminoallyl-dU is a uridine-based amino-modifier phosphoramidite. It is an excellent building block used for the synthesis of amine-modified oligonucleotides and DNAs. Placing the amino group farther from the ribose may be advantageous in post-synthetic reactions. Coupling is accomplished using normal dilution and protocols for oligonucleotides. It is an extremely robust method to introduce an amino group into oligonucleotides or DNAs that can be readily conjugated to a tag (such as a fluorescent dye, an enzyme or other biological tags) used in a variety of biological detections.

Calculators

Common stock solution preparation

Table 1. Volume of MeCN needed to reconstitute specific mass of 5-Aminoallyl-dU Phosphoramidite [5-Aminoallyl-dU CEP] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	113.393 µL	566.964 µL	1.134 mL	5.67 mL	11.339 mL
5 mM	22.679 µL	113.393 µL	226.786 µL	1.134 mL	2.268 mL
10 mM	11.339 µL	56.696 µL	113.393 µL	566.964 µL	1.134 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	881.89 g/mol	=	mL	x	mM	=	mmol

References

View all 9 references: Citation Explorer

Investigation and validation of labelling loop mediated isothermal amplification (LAMP) products with different nucleotide modifications for various downstream analysis.

Authors:

Warmt, Christian and Yaslanmaz, Ceren and Henkel, Jörg

Journal:

Scientific reports (2022): 7137

Round A/Round B Amplification of DNA for Microarrays.

Authors:

Rando, Oliver

Journal:

Cold Spring Harbor protocols (2019)

Indirect Aminoallyl-dUTP Labeling of RNA for Microarrays.

Authors:

Rando, Oliver

Journal:

Cold Spring Harbor protocols (2019)

A dual colour FISH method for routine validation of sexed Bos taurus semen.

Authors:

Reinsalu, Olavi and Scheler, Ott and Mikelsaar, Ruth and Mikelsaar, Aavo-Valdur and Hallap, Triin and Jaakma, Ülle and Padrik, Peeter and Kavak, Ants and Salumets, Andres and Kurg, Ants

Journal:

BMC veterinary research (2019): 104

Preparation of fluorescent-dye-labeled cDNA from RNA for microarray hybridization.

Authors:

Ares, Manuel

Journal:

Cold Spring Harbor protocols (2014): 119-23

Physical properties

Molecular weight	881.89
Solvent	MeCN

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
CAS	144253-90-7

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Page updated on May 8, 2026