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5-Aminoallyl-dU Phosphoramidite [5-Aminoallyl-dU CEP]

Product key features

  • A robust building block for preparing amino-modified oligonucleotides
  • Mild coupling conditions
  • Readily deprotected

Product description

5-Aminoallyl-dU is a uridine-based amino-modifier phosphoramidite. It is an excellent building block used for the synthesis of amine-modified oligonucleotides and DNAs. Placing the amino group farther from the ribose may be advantageous in post-synthetic reactions. Coupling is accomplished using normal dilution and protocols for oligonucleotides. It is an extremely robust method to introduce an amino group into oligonucleotides or DNAs that can be readily conjugated to a tag (such as a fluorescent dye, an enzyme or other biological tags) used in a variety of biological detections.

References

View all 9 references: Citation Explorer
Investigation and validation of labelling loop mediated isothermal amplification (LAMP) products with different nucleotide modifications for various downstream analysis.
Authors: Warmt, Christian and Yaslanmaz, Ceren and Henkel, Jörg
Journal: Scientific reports (2022): 7137
Round A/Round B Amplification of DNA for Microarrays.
Authors: Rando, Oliver
Journal: Cold Spring Harbor protocols (2019)
Indirect Aminoallyl-dUTP Labeling of RNA for Microarrays.
Authors: Rando, Oliver
Journal: Cold Spring Harbor protocols (2019)
A dual colour FISH method for routine validation of sexed Bos taurus semen.
Authors: Reinsalu, Olavi and Scheler, Ott and Mikelsaar, Ruth and Mikelsaar, Aavo-Valdur and Hallap, Triin and Jaakma, Ülle and Padrik, Peeter and Kavak, Ants and Salumets, Andres and Kurg, Ants
Journal: BMC veterinary research (2019): 104
Preparation of fluorescent-dye-labeled cDNA from RNA for microarray hybridization.
Authors: Ares, Manuel
Journal: Cold Spring Harbor protocols (2014): 119-23
Page updated on September 17, 2025

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Catalog Number6207
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Physical properties

Molecular weight

881.89

Solvent

MeCN

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure

CAS

144253-90-7