Name: 5-(and-6)-Carboxy SNARF-1
Brand: AAT Bioquest
SKU: 21226
Price: 352 USD
Availability: InStock

5-(and-6)-Carboxy SNARF-1

5-(and-6)-Carboxy SNARF-1 is an excellent pH probe for monitoring pH changes in the range of 6.5 and 8.5. It has pKa of ≤7.5. It exhibits a significant pH-dependent emission shift from yellow orange to deep red fluorescence under acidic and basic conditions, respectively. The ratioable pH dependent fluorescence intensity change allows the ratio of the fluorescence intensities from the dye at two emission wavelengths - typically 580 nm and 640 nm - to be used for quantitative determinations of pH in cells and other biological media.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21226	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-(and-6)-Carboxy SNARF-1 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	220.531 µL	1.103 mL	2.205 mL	11.027 mL	22.053 mL
5 mM	44.106 µL	220.531 µL	441.063 µL	2.205 mL	4.411 mL
10 mM	22.053 µL	110.266 µL	220.531 µL	1.103 mL	2.205 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	453.45 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Reducing the Bitter Taste of Pharmaceuticals Using Cell-Based Identification of Bitter-Masking Compounds.

Authors:

Beltrán, Leopoldo Raul and Sterneder, Sonja and Hasural, Ayse and Paetz, Susanne and Hans, Joachim and Ley, Jakob Peter and Somoza, Veronika

Journal:

Pharmaceuticals (Basel, Switzerland) (2022)

Ocean acidification impacts sperm swimming performance and pHi in the New Zealand sea urchin Evechinus chloroticus.

Authors:

Hudson, Michael E and Sewell, Mary A

Journal:

The Journal of experimental biology (2022)

Durability of Implanted Low-Density Polyacrylamide Hydrogel Used as a Scaffold for Microencapsulated Molecular Probes inside Small Fish.

Authors:

Shchapova, Ekaterina and Titov, Evgeniy and Gurkov, Anton and Nazarova, Anna and Borvinskaya, Ekaterina and Timofeyev, Maxim

Journal:

Polymers (2022)

Calibration and measurement of mitochondrial pH in intact adult rat cardiomyocytes.

Authors:

Gao, Meng and Qin, Yuan and Li, Anqi and Liu, Hanyu and Chen, Lei and Liu, Bilin and Zhang, Ying and Gao, Yufei and Gong, Guohua

Journal:

STAR protocols (2021): 100543

Studying Proton Gradients Across the Nuclear Envelope.

Authors:

Martínez-Zaguilán, Raul and Sennoune, Souad R

Journal:

Methods in molecular biology (Clifton, N.J.) (2020): 47-63

Physical properties

Molecular weight	453.45
Solvent	DMSO

Spectral properties

Excitation (nm)	549
Emission (nm)	587

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on December 5, 2025