5-(and-6)-Carboxy SNARF-4F AM Ester

5-(and-6)-Carboxy SNARF-4F AM Ester is an excellent cell-permeable pH probes for ratiometrically monitoring pH changes in live cells. SNARF-4F has pKa of ∼6.4. It exhibits a significant pH-dependent emission shift from yellow orange to deep red fluorescence under acidic and basic conditions, respectively. The ratioable pH dependent fluorescence intensity change allows the ratio of the fluorescence intensities from the dye at two emission wavelengths - typically 580 nm and 640 nm - to be used for quantitative determinations of pH in cells and other biological media.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21173	10x50 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-(and-6)-Carboxy SNARF-4F AM Ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	170.783 µL	853.913 µL	1.708 mL	8.539 mL	17.078 mL
5 mM	34.157 µL	170.783 µL	341.565 µL	1.708 mL	3.416 mL
10 mM	17.078 µL	85.391 µL	170.783 µL	853.913 µL	1.708 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	585.54 g/mol	=	mL	x	mM	=	mmol

References

View all 17 references: Citation Explorer

Biofilm-responsive encapsulated-phage coating for autonomous biofouling mitigation in water storage systems.

Authors:

Zuo, Pengxiao and Metz, Jordin and Yu, Pingfeng and Alvarez, Pedro J J

Journal:

Water research (2022): 119070

Non-Destructive Measurement of Acetic Acid and Its Distribution in a Photovoltaic Module during Damp Heat Testing Using pH-Sensitive Fluorescent Dye Sensors.

Authors:

Nagasaki, Hideaki and Asaka, Takashi and Iwami, Kentaro and Umeda, Norihiro and Yamamoto, Chizuko and Hara, Yukiko and Masuda, Atsushi

Journal:

Sensors (Basel, Switzerland) (2022)

Evaluation of Interfacial pH Using Surface Forces Apparatus Fluorescence Spectroscopy.

Authors:

Kasuya, Motohiro and Sano, Yuka and Kawashima, Masataka and Kurihara, Kazue

Journal:

Langmuir : the ACS journal of surfaces and colloids (2021): 5073-5080

Identifying the localization and exploring a functional role for Gprc5c in the kidney.

Authors:

Rajkumar, Premraj and Cha, Boyoung and Yin, Jianyi and Arend, Lois J and Păunescu, Teodor G and Hirabayashi, Yoshio and Donowitz, Mark and Pluznick, Jennifer L

Journal:

FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2018): 2046-2059

Both NHERF3 and NHERF2 are necessary for multiple aspects of acute regulation of NHE3 by elevated Ca2+, cGMP, and lysophosphatidic acid.

Authors:

Avula, Leela Rani and Chen, Tiane and Kovbasnjuk, Olga and Donowitz, Mark

Journal:

American journal of physiology. Gastrointestinal and liver physiology (2018): G81-G90

Physical properties

Molecular weight	585.54
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on December 6, 2025