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5-(and-6)-Carboxy SNARF-5F AM Ester

5-(and-6)-Carboxy SNARF-5F AM Ester is an excellent cell-permeable pH probes for ratiometrically monitoring pH changes in live cells. SNARF-5F has pKa of ∼7.2. It exhibits a significant pH-dependent emission shift from yellow orange to deep red fluorescence under acidic and basic conditions, respectively. The ratioable pH dependent fluorescence intensity change allows the ratio of the fluorescence intensities from the dye at two emission wavelengths - typically 580 nm and 640 nm - to be used for quantitative determinations of pH in cells and other biological media.

References

View all 50 references: Citation Explorer
Combined pH ratiometry and fluorescence lectin-binding analysis (pH-FLBA) for microscopy-based analyses of biofilm pH and matrix carbohydrates.
Authors: Del Rey, Yumi C and Schramm, Andreas and L Meyer, Rikke and Lund, Marie Braad and Schlafer, Sebastian
Journal: Applied and environmental microbiology (2024): e0200723
In vivo simultaneous nonlinear absorption Raman and fluorescence (SNARF) imaging of mouse brain cortical structures.
Authors: Francis, Andrew T and Manifold, Bryce and Carlson, Elena C and Hu, Ruoqian and Hill, Andrew H and Men, Shuaiqian and Fu, Dan
Journal: Communications biology (2022): 222
Non-Destructive Measurement of Acetic Acid and Its Distribution in a Photovoltaic Module during Damp Heat Testing Using pH-Sensitive Fluorescent Dye Sensors.
Authors: Nagasaki, Hideaki and Asaka, Takashi and Iwami, Kentaro and Umeda, Norihiro and Yamamoto, Chizuko and Hara, Yukiko and Masuda, Atsushi
Journal: Sensors (Basel, Switzerland) (2022)
Stimuli-Responsible SNARF Derivatives as a Latent Ratiometric Fluorescent Probe.
Authors: Nakata, Eiji and Gerelbaatar, Khongorzul and Komatsubara, Futa and Morii, Takashi
Journal: Molecules (Basel, Switzerland) (2022)
Evaluation of Interfacial pH Using Surface Forces Apparatus Fluorescence Spectroscopy.
Authors: Kasuya, Motohiro and Sano, Yuka and Kawashima, Masataka and Kurihara, Kazue
Journal: Langmuir : the ACS journal of surfaces and colloids (2021): 5073-5080
Page updated on May 19, 2025

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Catalog Number21248
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Physical properties

Molecular weight

585.54

Solvent

DMSO

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
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