5-(and-6)-Carboxy SNARF-5F Succinimidyl Ester

5-(and-6)-Carboxy SNARF-5F Succinimidyl Ester is an excellent amine-reactive building block that can be readily used for developing SNARF-5F pH probes. SNARF-5F has pKa of ∼7.2. It exhibits a significant pH-dependent emission shift from yellow orange to deep red fluorescence under acidic and basic conditions, respectively. The ratioable pH dependent fluorescence intensity change allows the ratio of the fluorescence intensities from the dye at two emission wavelengths - typically 580 nm and 640 nm - to be used for quantitative determinations of pH in cells and other biological media.

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
21246	10x50 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-(and-6)-Carboxy SNARF-5F Succinimidyl Ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	175.898 µL	879.492 µL	1.759 mL	8.795 mL	17.59 mL
5 mM	35.18 µL	175.898 µL	351.797 µL	1.759 mL	3.518 mL
10 mM	17.59 µL	87.949 µL	175.898 µL	879.492 µL	1.759 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	568.51 g/mol	=	mL	x	mM	=	mmol

References

View all 11 references: Citation Explorer

Hyperpolarization induces cytosolic alkalization of mouse sperm flagellum probably through sperm Na+/H+ exchanger.

Authors:

Hernández-Garduño, Sandra and Chávez, Julio C and Matamoros-Volante, Arturo and Sánchez-Guevara, Yoloxochitl and Torres, Paulina and Treviño, Claudia L and Nishigaki, Takuya

Journal:

Reproduction (Cambridge, England) (2022): 125-134

Quantitative Intracellular pH Determinations in Single Live Mammalian Spermatozoa Using the Ratiometric Dye SNARF-5F.

Authors:

Chávez, Julio C and Darszon, Alberto and Treviño, Claudia L and Nishigaki, Takuya

Journal:

Frontiers in cell and developmental biology (2019): 366

In vivo quantitative imaging of tumor pH by nanosonophore assisted multispectral photoacoustic imaging.

Authors:

Jo, Janggun and Lee, Chang H and Kopelman, Raoul and Wang, Xueding

Journal:

Nature communications (2017): 471

Circulating aldosterone induces the apical accumulation of the proton pumping V-ATPase and increases proton secretion in clear cells in the caput epididymis.

Authors:

Roy, Jeremy W and Hill, Eric and Ruan, Ye Chun and Vedovelli, Luca and Păunescu, Teodor G and Brown, Dennis and Breton, Sylvie

Journal:

American journal of physiology. Cell physiology (2013): C436-46

Pixels as ROIs (PAR): a less-biased and statistically powerful approach for gleaning functional information from image stacks.

Authors:

Keller, Jacob and Homma, Kazuaki and Dallos, Peter

Journal:

PloS one (2013): e69047

Physical properties

Molecular weight	568.51
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on February 7, 2026