5-Carboxy-4'-aminomethylfluorescein

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Physical properties

Molecular weight	441.82
Solvent	DMSO

Spectral properties

Excitation (nm)	498
Emission (nm)	525

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

441.82

Excitation (nm)

498

Emission (nm)

525

5-Carboxy-4'-aminomethylfluorescein is a key building block for developing Big Dye DNA sequencing terminators. It can also be incorporated into a peptide sequence to give fluoresceinated peptides.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-Carboxy-4'-aminomethylfluorescein to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	226.337 µL	1.132 mL	2.263 mL	11.317 mL	22.634 mL
5 mM	45.267 µL	226.337 µL	452.673 µL	2.263 mL	4.527 mL
10 mM	22.634 µL	113.168 µL	226.337 µL	1.132 mL	2.263 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Excitation (nm)	498
Emission (nm)	525

Product Family

Name	Excitation (nm)	Emission (nm)
6-Carboxy-4'-aminomethylfluorescein	498	525

Images

Figure 1. Chemical structure for 5-Carboxy-4'-aminomethylfluorescein

References

View all 65 references: Citation Explorer

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Journal: Langmuir (2006): 5877

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Journal: Aliment Pharmacol Ther (2006): 1649

Investigation of the role of ENPP1 and TNAP genes in chondrocalcinosis
Authors: Zhang Y, Brown MA, Peach C, Russell G, Wordsworth BP.
Journal: Rheumatology (Oxford). (2006)

Potentials of high-temperature anaerobic treatment and redox mediators for the reductive decolorization of azo dyes from textile wastewaters
Authors: Dos Santos AB, Cervantes FJ, van Lier JB.
Journal: Water Sci Technol (2006): 151

Genotypes of the cytochrome p450 isoform, CYP2C9, and the vitamin K epoxide reductase complex subunit 1 conjointly determine stable warfarin dose: a prospective study
Authors: Carlquist JF, Horne BD, Muhlestein JB, Lappe DL, Whiting BM, Kolek MJ, Clarke JL, James BC, Anderson JL.
Journal: J Thromb Thrombolysis. (2006)

Relation between intrinsic connections and isofrequency contours in the inferior colliculus of the big brown bat, Eptesicus fuscus
Authors: Miller KE, Casseday JH, Covey E.
Journal: Neuroscience (2005): 895