AAT Bioquest


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Physical properties
Molecular weight441.82
Spectral properties
Excitation (nm)498
Emission (nm)525
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
5-Carboxy-4'-aminomethylfluorescein is a key building block for developing Big Dye DNA sequencing terminators. It can also be incorporated into a peptide sequence to give fluoresceinated peptides.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-Carboxy-4'-aminomethylfluorescein to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM226.337 µL1.132 mL2.263 mL11.317 mL22.634 mL
5 mM45.267 µL226.337 µL452.673 µL2.263 mL4.527 mL
10 mM22.634 µL113.168 µL226.337 µL1.132 mL2.263 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectral properties

Excitation (nm)498
Emission (nm)525

Product Family

NameExcitation (nm)Emission (nm)



View all 65 references: Citation Explorer
UV Raman spectroscopic study on TiO2. I. Phase transformation at the surface and in the bulk
Authors: Zhang J, Li M, Feng Z, Chen J, Li C.
Journal: J Phys Chem B Condens Matter Mater Surf Interfaces Biophys (2006): 927
Genotypes of the cytochrome p450 isoform, CYP2C9, and the vitamin K epoxide reductase complex subunit 1 conjointly determine stable warfarin dose: a prospective study
Authors: Carlquist JF, Horne BD, Muhlestein JB, Lappe DL, Whiting BM, Kolek MJ, Clarke JL, James BC, Anderson JL.
Journal: J Thromb Thrombolysis. (2006)
Potentials of high-temperature anaerobic treatment and redox mediators for the reductive decolorization of azo dyes from textile wastewaters
Authors: Dos Santos AB, Cervantes FJ, van Lier JB.
Journal: Water Sci Technol (2006): 151
Investigation of the role of ENPP1 and TNAP genes in chondrocalcinosis
Authors: Zhang Y, Brown MA, Peach C, Russell G, Wordsworth BP.
Journal: Rheumatology (Oxford). (2006)
Intrahepatic cholestasis of pregnancy: three novel MDR3 gene mutations
Authors: Floreani A, Carderi I, Paternoster D, Soardo G, Azzaroli F, Esposito W, Variola A, Tommasi AM, Marchesoni D, Braghin C, Mazzella G.
Journal: Aliment Pharmacol Ther (2006): 1649
Size effect on the fluorescence properties of dansyl-doped silica nanoparticles
Authors: Montalti M, Prodi L, Zaccheroni N, Battistini G, Marcuz S, Mancin F, Rampazzo E, Tonellato U.
Journal: Langmuir (2006): 5877
Hepatitis delta virus (HDV) genotypes in patients with chronic hepatitis: molecular epidemiology of HDV in Turkey
Authors: Altuglu I, Ozacar T, Sertoz RY, Erensoy S.
Journal: Int J Infect Dis. (2006)
Polymorphisms and resistance mutations in the protease and reverse transcriptase genes of HIV-1 F subtype Romanian strains
Authors: Paraschiv S, Otelea D, Dinu M, Maxim D, Tinischi M.
Journal: Int J Infect Dis. (2006)
Voltage- and Ca2+-activated potassium channels in Ca2+ store control Ca2+ release
Authors: Yamashita M, Sugioka M, Ogawa Y.
Journal: Febs J (2006): 3585
Relation between intrinsic connections and isofrequency contours in the inferior colliculus of the big brown bat, Eptesicus fuscus
Authors: Miller KE, Casseday JH, Covey E.
Journal: Neuroscience (2005): 895