6-Carboxy-4'-aminomethylfluorescein

6-Carboxy-4'-aminomethylfluorescein is a key building block for developing Big Dye DNA sequencing terminators. It can also be incorporated into a peptide sequence to give fluoresceinated peptides.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
208	10 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-Carboxy-4'-aminomethylfluorescein to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	226.337 µL	1.132 mL	2.263 mL	11.317 mL	22.634 mL
5 mM	45.267 µL	226.337 µL	452.673 µL	2.263 mL	4.527 mL
10 mM	22.634 µL	113.168 µL	226.337 µL	1.132 mL	2.263 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	441.82 g/mol	=	mL	x	mM	=	mmol

References

View all 65 references: Citation Explorer

UV Raman spectroscopic study on TiO2. I. Phase transformation at the surface and in the bulk

Authors:

Zhang J, Li M, Feng Z, Chen J, Li C.

Journal:

J Phys Chem B Condens Matter Mater Surf Interfaces Biophys (2006): 927

Genotypes of the cytochrome p450 isoform, CYP2C9, and the vitamin K epoxide reductase complex subunit 1 conjointly determine stable warfarin dose: a prospective study

Authors:

Carlquist JF, Horne BD, Muhlestein JB, Lappe DL, Whiting BM, Kolek MJ, Clarke JL, James BC, Anderson JL.

Journal:

J Thromb Thrombolysis. (2006)

Potentials of high-temperature anaerobic treatment and redox mediators for the reductive decolorization of azo dyes from textile wastewaters

Authors:

Dos Santos AB, Cervantes FJ, van Lier JB.

Journal:

Water Sci Technol (2006): 151

Investigation of the role of ENPP1 and TNAP genes in chondrocalcinosis

Authors:

Zhang Y, Brown MA, Peach C, Russell G, Wordsworth BP.

Journal:

Rheumatology (Oxford). (2006)

Intrahepatic cholestasis of pregnancy: three novel MDR3 gene mutations

Authors:

Floreani A, Carderi I, Paternoster D, Soardo G, Azzaroli F, Esposito W, Variola A, Tommasi AM, Marchesoni D, Braghin C, Mazzella G.

Journal:

Aliment Pharmacol Ther (2006): 1649

Physical properties

Molecular weight	441.82
Solvent	DMSO

Spectral properties

Excitation (nm)	498
Emission (nm)	525

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 13, 2025