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5-CFDA, AM [5-Carboxyfluorescein Diacetate, Acetoxymethyl Ester]

5-Carboxyfluorescein diacetate, acetoxymethyl ester (CFDA, AM) is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is required to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Upon hydrolysis by intracellular esterases, CFDA, AM yields the 5-carboxyfluorescein product that gives bright green fluorescence when excited with a blue light or the common 488 nm Argon laser. It is often used to monitor cell viability or label live cells for cell analysis.

References

View all 50 references: Citation Explorer
Sodium Oligomannate Electrostatically Binds to Aβ and Blocks Its Aggregation.
Authors: Zhou, Chen and Zhang, Jingjing and Luo, Xinwen and Lian, Fulin and Zeng, Yangyang and Zhang, Zhenqing and Zhang, Haiyan and Zhang, Naixia
Journal: The journal of physical chemistry. B (2023): 1983-1994
A Zn(II) coordination polymer as a dual sensor for ppb level detection of antibiotics and organo-toxins in a green solvent.
Authors: Rawat, Ashutosh and Kanzariya, Dashrathbhai B and Lama, Prem and Pal, Tapan K
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2023): 122579
Treatment effects of pulmonary artery denervation for pulmonary arterial hypertension stratified by REVEAL risk score: Results from PADN-CFDA trial.
Authors: Zhang, Juan and Kan, Jing and Wei, Yongyue and Zhang, Caojin and Yang, Zhenwen and Gu, Heping and Fan, Fenling and Gu, Hong and Wang, Qiguang and Xie, Dujiang and Zhang, Gangcheng and Guo, Xiaomei and Yin, Yuehui and Jin, Bowen and Zhou, Hongmei and Yang, Ziyang and Wang, Zhouming and Xin, Yu and Zhang, Chen and Meng, Lili and Wang, Xiaoyu and Zhao, Chunxia and Zhang, Hang and Yan, Xiaoyan and Chen, Feng and Yao, Cheng and Benza, Raymond L and Stone, Gregg W and Chen, Shao-Liang
Journal: The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation (2023)
Research Progress of Immunotherapy for Gastric Cancer.
Authors: Zhang, Zhipeng and Liu, Ningning and Sun, Mingyu
Journal: Technology in cancer research & treatment (2023): 15330338221150555
Cytotoxicity Assays with Zebrafish Cell Lines.
Authors: Rodrigues de Souza, Irisdoris and Wilke Sivek, Tainá and Vaz de Oliveira, Júlia Beatriz and Di Pietro Micali Canavez, Andrezza and de Albuquerque Vita, Natália and Cigaran Schuck, Desiree and Rodrigues de Souza, Isisdoris and Cestari, Marta Margarete and Lorencini, Marcio and Leme, Daniela Morais
Journal: Journal of visualized experiments : JoVE (2023)
Page updated on December 13, 2024

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Catalog Number21910
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Physical properties

Molecular weight

532.46

Solvent

DMSO

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure

CAS

124412-00-6
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