AAT Bioquest

5-dR110, succinimidyl ester

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Physical properties
Molecular weight540.31
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Compared to fluorescein labeling reagents such as FITC and FAM, 5-dR110 [5-carboxy-4,7-dichlororhodamine 110] reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength. They are photostable alternative reagents superior to FITC and FAM. 5-carboxy-4,7-dichlororhodamine (dR110) is one of the four critical 4,7-dichloro-rhodamine dyes used as the BigDye terminators for DNA sequencing in combination with d6G, dTMR and dROX. ABI PRISM® BigDye™ Terminator Cycle Sequencing is the classic method for DNA sequencing. 5-dR110, succinimidyl ester is the most convenient building block for preparing the dR110 color of BigDye Sanger sequencing fluorophore set.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-dR110, succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM185.079 µL925.395 µL1.851 mL9.254 mL18.508 mL
5 mM37.016 µL185.079 µL370.158 µL1.851 mL3.702 mL
10 mM18.508 µL92.539 µL185.079 µL925.395 µL1.851 mL

Molarity calculator

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