5-dR6G [5-Carboxy-4,7-dichlororhodamine 6G]

5-dR6G

5-Carboxy-4,7-dichlororhodamine 6G

5-dR6G (5-Carboxy-4,7-dichlororhodamine 6G) is one of the four critical 4,7-dichloro-rhodamine dyes used as the BigDye terminators for DNA sequencing in combination with dR110, dTMR and dROX. ABI PRISM® BigDye™ Terminator Cycle Sequencing is the classic method for DNA sequencing.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
300	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-dR6G [5-Carboxy-4,7-dichlororhodamine 6G] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	159.086 µL	795.431 µL	1.591 mL	7.954 mL	15.909 mL
5 mM	31.817 µL	159.086 µL	318.172 µL	1.591 mL	3.182 mL
10 mM	15.909 µL	79.543 µL	159.086 µL	795.431 µL	1.591 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	628.59 g/mol	=	mL	x	mM	=	mmol

References

View all 6 references: Citation Explorer

Design and synthesis of fluorescence-labeled nucleotide with a cleavable azo linker for DNA sequencing

Authors:

Tan, L.; Liu, Y.; Yang, Q.; Li, X.; Wu, X. Y.; Gong, B.; Shen, Y. M.; Shao, Z.

Journal:

Chem Commun (Camb) (2016): 954-7

Synthesis of novel tyrosinyl FRET cassettes, terminators, and their potential use in DNA sequencing

Authors:

Sudhakar Rao, T.; Zhang, W.; Xiao, H.; Flick, P.; Kumar, S.; Nampalli, S.

Journal:

Nucleosides Nucleotides Nucleic Acids (2003): 1443-5

Design strategies and performance of custom DNA sequencing primers

Authors:

Buck, G. A.; Fox, J. W.; Gunthorpe, M.; Hager, K. M.; Naeve, C. W.; Pon, R. T.; Adams, P. S.; Rush, J.

Journal:

Biotechniques (1999): 528-36

Improved DNA sequencing accuracy and detection of heterozygous alleles using manganese citrate and different fluorescent dye terminators

Authors:

Korch, C.; Drabkin, H.

Journal:

Genome Res (1999): 588-95

Peak height pattern in dichloro-rhodamine and energy transfer dye terminator sequencing

Authors:

Zakeri, H.; Amparo, G.; Chen, S. M.; Spurgeon, S.; Kwok, P. Y.

Journal:

Biotechniques (1998): 406-10, 412-4

Physical properties

Molecular weight	628.59
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 6, 2025