AAT Bioquest

5-dR6G, succinimidyl ester

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Physical properties
Molecular weight624.47
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
5-dR6G (5-Carboxy-4,7-dichlororhodamine 6G) is one of the four critical 4,7-dichloro-rhodamine dyes used as the BigDye terminators for DNA sequencing in combination with dR110, dTMR and dROX. ABI PRISM® BigDye™ Terminator Cycle Sequencing is the classic method for DNA sequencing. 5-dR6G, succinimidyl ester is the most convenient building block for preparing the dR6G color of BigDye Sanger sequencing fluorophore set.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-dR6G, succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM160.136 µL800.679 µL1.601 mL8.007 mL16.014 mL
5 mM32.027 µL160.136 µL320.272 µL1.601 mL3.203 mL
10 mM16.014 µL80.068 µL160.136 µL800.679 µL1.601 mL

Molarity calculator

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View all 2 references: Citation Explorer
Improved DNA sequencing accuracy and detection of heterozygous alleles using manganese citrate and different fluorescent dye terminators.
Authors: Korch, C and Drabkin, H
Journal: Genome research (1999): 588-95
Peak height pattern in dichloro-rhodamine and energy transfer dye terminator sequencing.
Authors: Zakeri, H and Amparo, G and Chen, S M and Spurgeon, S and Kwok, P Y
Journal: BioTechniques (1998): 406-10, 412-4