AAT Bioquest

5-dROX, succinimidyl ester

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Physical properties
Molecular weight700.57
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
5-dROX (5-Carboxy-4,7-dichloror-X-hodamine) is one of the four critical 4,7-dichloro-rhodamine dyes used as the BigDye terminators for DNA sequencing in combination with dR110, dR6G and dROX. ABI PRISM® BigDye™ Terminator Cycle Sequencing is the classic method for DNA sequencing. 5-dROX, succinimidyl ester is the most convenient building block for preparing the dROX color of BigDye Sanger sequencing fluorophore set.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-dROX, succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM142.741 µL713.705 µL1.427 mL7.137 mL14.274 mL
5 mM28.548 µL142.741 µL285.482 µL1.427 mL2.855 mL
10 mM14.274 µL71.37 µL142.741 µL713.705 µL1.427 mL

Molarity calculator

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View all 2 references: Citation Explorer
Improved DNA sequencing accuracy and detection of heterozygous alleles using manganese citrate and different fluorescent dye terminators.
Authors: Korch, C and Drabkin, H
Journal: Genome research (1999): 588-95
Peak height pattern in dichloro-rhodamine and energy transfer dye terminator sequencing.
Authors: Zakeri, H and Amparo, G and Chen, S M and Spurgeon, S and Kwok, P Y
Journal: BioTechniques (1998): 406-10, 412-4