5-dROX, succinimidyl ester

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Physical properties

Molecular weight	700.57
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

700.57

5-dROX (5-Carboxy-4,7-dichloror-X-hodamine) is one of the four critical 4,7-dichloro-rhodamine dyes used as the BigDye terminators for DNA sequencing in combination with dR110, dR6G and dROX. ABI PRISM® BigDye™ Terminator Cycle Sequencing is the classic method for DNA sequencing. 5-dROX, succinimidyl ester is the most convenient building block for preparing the dROX color of BigDye Sanger sequencing fluorophore set.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-dROX, succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	142.741 µL	713.705 µL	1.427 mL	7.137 mL	14.274 mL
5 mM	28.548 µL	142.741 µL	285.482 µL	1.427 mL	2.855 mL
10 mM	14.274 µL	71.37 µL	142.741 µL	713.705 µL	1.427 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Images

Figure 1. Chemical structure for 5-dROX, succinimidyl ester.

References

View all 2 references: Citation Explorer

Improved DNA sequencing accuracy and detection of heterozygous alleles using manganese citrate and different fluorescent dye terminators.
Authors: Korch, C and Drabkin, H
Journal: Genome research (1999): 588-95

Peak height pattern in dichloro-rhodamine and energy transfer dye terminator sequencing.
Authors: Zakeri, H and Amparo, G and Chen, S M and Spurgeon, S and Kwok, P Y
Journal: BioTechniques (1998): 406-10, 412-4