AAT Bioquest

5-dTMR, succinimidyl ester

Product Image
Product Image
Gallery Image 1
Ordering information
Catalog Number
Unit Size
Add to cart
Additional ordering information
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight596.42
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
5-dTMR (5-Carboxy-4,7-dichlorortetramethylrhodamine) is one of the four critical 4,7-dichloro-rhodamine dyes used as the BigDye terminators for DNA sequencing in combination with dR110, dR6G and dROX. ABI PRISM® BigDye™ Terminator Cycle Sequencing is the classic method for DNA sequencing. 5-dTMR, succinimidyl ester is the most convenient building block for preparing the dTMR color of BigDye Sanger sequencing fluorophore set.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-dTMR, succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM167.667 µL838.335 µL1.677 mL8.383 mL16.767 mL
5 mM33.533 µL167.667 µL335.334 µL1.677 mL3.353 mL
10 mM16.767 µL83.834 µL167.667 µL838.335 µL1.677 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles



View all 2 references: Citation Explorer
Improved DNA sequencing accuracy and detection of heterozygous alleles using manganese citrate and different fluorescent dye terminators.
Authors: Korch, C and Drabkin, H
Journal: Genome research (1999): 588-95
Peak height pattern in dichloro-rhodamine and energy transfer dye terminator sequencing.
Authors: Zakeri, H and Amparo, G and Chen, S M and Spurgeon, S and Kwok, P Y
Journal: BioTechniques (1998): 406-10, 412-4