5-FAM [5-Carboxyfluorescein] *CAS 76823-03-5*

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Chemical structure for 5-FAM [5-Carboxyfluorescein] *CAS 76823-03-5*
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Overview

Ex/Em (nm)494/521
MW376.32
CAS #76823-03-5
SolventDMSO
Storage F/D/L
Category Peptide Synthesis
Peptide Labeling Reagents
Related Carbonyl-Reactive Biotins
Amine-Reactive Probes
Secondary Reagents
5-FAM is the purified single isomer of carboxyfluorescein. It is one of the most popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. It has been predominantly used to develop a variety of green fluorescent peptides that can be excited with the 488 nm line of the Ar laser. It has also been used to prepare various small fluorescent molecules.




Calculators
Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-FAM [5-Carboxyfluorescein] *CAS 76823-03-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.



Molarity calculator

Table 2. Enter any two values (mass, volume, concentration) to calculate the third.

Mass Molecular weight Volume Concentration Moles
/ = x =
 






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References & Citations

Multicellular Vascularized Engineered Tissues through User-Programmable Biomaterial Photodegradation
Authors: Christopher K Arakawa, Barry A Badeau, Ying Zheng, Cole A DeForest
Journal: Advanced Materials (2017)

Green tea inhibited the elimination of nephro-cardiovascular toxins and deteriorated the renal function in rats with renal failure
Authors: Yu-Hsuan Peng, Douglas H Sweet, Shiuan-Pey Lin, Chung-Ping Yu, Pei-Dawn Lee Chao, Yu-Chi Hou
Journal: Scientific reports (2015)

Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Søren Preus, L Marcus Wilhelmsson
Journal: ChemBioChem (2012): 1990--2001






Additional Documents

 
Safety Data Sheet (SDS)


Catalogs
1. Oligonucleotide Labeling Reagents
2. Peptide Labeling Reagents

Certificate of Analysis