5-FAM | AAT Bioquest

5-FAM

5-Carboxyfluorescein; CAS 76823-03-5

5-FAM is the purified single isomer of carboxyfluorescein. It is one of the most popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. It has been predominantly used to develop a variety of green fluorescent peptides that can be excited with the 488 nm line of the Ar laser. It has also been used to prepare various small fluorescent molecules.

Protocol

COO

COA

Catalog	Size	Price
103	100 mg	Price
104	1 g	Price
105	5 g	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-FAM [5-Carboxyfluorescein] *CAS 76823-03-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	265.731 µL	1.329 mL	2.657 mL	13.287 mL	26.573 mL
5 mM	53.146 µL	265.731 µL	531.463 µL	2.657 mL	5.315 mL
10 mM	26.573 µL	132.866 µL	265.731 µL	1.329 mL	2.657 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	376.32 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

Multicellular Vascularized Engineered Tissues through User-Programmable Biomaterial Photodegradation

Authors:

Arakawa, Christopher K and Badeau, Barry A and Zheng, Ying and DeForest, Cole A

Journal:

Advanced Materials (2017)

Green tea inhibited the elimination of nephro-cardiovascular toxins and deteriorated the renal function in rats with renal failure

Authors:

Peng, Yu-Hsuan and Sweet, Douglas H and Lin, Shiuan-Pey and Yu, Chung-Ping and Chao, Pei-Dawn Lee and Hou, Yu-Chi

Journal:

Scientific reports (2015)

Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids

Authors:

Preus, Søren and Wilhelmsson, L Marcus

Journal:

ChemBioChem (2012): 1990--2001

References

View all 137 references: Citation Explorer

Carboxyfluorescein leakage from poly(ethylene glycol)-grafted liposomes induced by the interaction with serum

Authors:

Hashizaki K, Taguchi H, Sakai H, Abe M, Saito Y, Ogawa N.

Journal:

Chem Pharm Bull (Tokyo) (2006): 80

Quantifying lymphocyte kinetics in vivo using carboxyfluorescein diacetate succinimidyl ester (cfse)

Authors:

Asquith B, Debacq C, Florins A, Gillet N, Sanchez-Alcaraz T, Mosley A, Willems L.

Journal:

Proc Biol Sci (2006): 1165

Evidence for dimer formation by an amphiphilic heptapeptide that mediates chloride and carboxyfluorescein release from liposomes

Authors:

Pajewski R, Ferdani R, Pajewska J, Djedovic N, Schlesinger PH, Gokel GW.

Journal:

Org Biomol Chem (2005): 619

Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling

Authors:

Wang XQ, Duan XM, Liu LH, Fang YQ, Tan Y.

Journal:

Acta Biochim Biophys Sin (Shanghai) (2005): 379

Low incidence of acute rejection after living-donor liver transplantation: Immunologic analyses by mixed lymphocyte reaction using a carboxyfluorescein diacetate succinimidyl ester labeling technique

Authors:

Tanaka Y, Ohdan H, Onoe T, Mitsuta H, Tashiro H, Itamoto T, Asahara T.

Journal:

Transplantation (2005): 1262

Physical properties

Molecular weight	376.32
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	83000
Excitation (nm)	493
Emission (nm)	517

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	76823-03-5

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Page updated on January 23, 2025