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5-FITC cadaverine

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Physical properties
Molecular weight653.38
SolventDMSO
Spectral properties
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)73000
Excitation (nm)491
Emission (nm)516
Quantum yield0.92
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


See also: Fluoresceins
Molecular weight
653.38
Correction Factor (280 nm)
0.35
Extinction coefficient (cm -1 M -1)
73000
Excitation (nm)
491
Emission (nm)
516
Quantum yield
0.92
FITC cadaverine is an excellent building block for developing fluoresceinated bioconjugates. It is also a fluorescent transglutaminase substrate to label proteins by transaminidation.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-FITC cadaverine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM153.05 µL765.251 µL1.531 mL7.653 mL15.305 mL
5 mM30.61 µL153.05 µL306.101 µL1.531 mL3.061 mL
10 mM15.305 µL76.525 µL153.05 µL765.251 µL1.531 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)73000
Excitation (nm)491
Emission (nm)516
Quantum yield0.92

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)Correction Factor (260 nm)
5-FITC ethylenediamine [Fluorescein thiocarbamylethylenediamine] *CAS 75453-82-6*491516730000.35-
5-FAM cadaverine493517830000.1780.32
5-TAMRA cadaverine552578900000.1780.32

Images


Citations


View all 2 citations: Citation Explorer
Mitochondrial-targeting Mn3O4/UIO-TPP nanozyme scavenge ROS to restore mitochondrial function for osteoarthritis therapy
Authors: Zhang, Shengqing and Cai, Jinhong and Yao, Yi and Huang, Lanli and Zheng, Li and Zhao, Jinmin
Journal: Regenerative Biomaterials (2023): rbad078
Investigating the Cell-Uptake of Guanidinium-Rich RAFT Polymers: Impact of Comonomer and Monomer Distribution
Authors: Martin, Liam and Peltier, Raoul and Kuroki, Agnes and Town, James and Perrier, Sebastien
Journal: Biomacromolecules (2018)

References


View all 14 references: Citation Explorer
Transglutaminase participates in UVB-induced cell death pathways in human corneal epithelial cells
Authors: Tong L, Chen Z, De Paiva CS, Beuerman R, Li DQ, Pflugfelder SC.
Journal: Invest Ophthalmol Vis Sci (2006): 4295
Human epidermal keratinocytes undergo (-)-epigallocatechin-3-gallate-dependent differentiation but not apoptosis
Authors: Balasubramanian S, Sturniolo MT, Dubyak GR, Eckert RL.
Journal: Carcinogenesis (2005): 1100
Transglutaminase activity is present in highly purified nonsynaptosomal mouse brain and liver mitochondria
Authors: Krasnikov BF, Kim SY, McConoughey SJ, Ryu H, Xu H, Stavrovskaya I, Iismaa SE, Mearns BM, Ratan RR, Blass JP, Gibson GE, Cooper AJ.
Journal: Biochemistry (2005): 7830
Proteolysis of cell-surface tissue transglutaminase by matrix metalloproteinase-2 contributes to the adhesive defect and matrix abnormalities in thrombospondin-2-null fibroblasts and mice
Authors: Agah A, Kyriakides TR, Bornstein P.
Journal: Am J Pathol (2005): 81
The development of a standardised protocol to measure squamous differentiation in stratified epithelia, by using the fluorescein cadaverine incorporation technique
Authors: Gray AC, Malton J, Clothier RH.
Journal: Altern Lab Anim (2004): 91
Importance of tissue transglutaminase in repair of extracellular matrices and cell death of dermal fibroblasts after exposure to a solarium ultraviolet A source
Authors: Gross SR, Balklava Z, Griffin M.
Journal: J Invest Dermatol (2003): 412
Enhanced expression of transglutaminase 2 in anterior polar cataracts and its induction by TGF-beta in vitro
Authors: Wan XH, Lee EH, Koh HJ, Song J, Kim EK, Kim CY, Lee JB, Kim SY, Yao K, Lee JH.
Journal: Br J Ophthalmol (2002): 1293
Tissue transglutaminase localization and activity regulation in the extracellular matrix of articular cartilage
Authors: Summey BT, Jr., Graff RD, Lai TS, Greenberg CS, Lee GM.
Journal: J Orthop Res (2002): 76
The use of an in vitro submerged keratinocyte model to predict induction of squamous metaplasia
Authors: Gray AC, Clothier RH.
Journal: Toxicol In Vitro (2001): 427
Characterization of tissue transglutaminase in human osteoblast-like cells
Authors: Heath DJ, Downes S, Verderio E, Griffin M.
Journal: J Bone Miner Res (2001): 1477