Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.  Learn more >>

5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7*

Chemical structure for 5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7*
Chemical structure for 5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7*
Ordering information
Price ()
Catalog Number120
Unit Size
Find Distributor
Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight389.38
SolventDMSO
Spectral properties
Correction Factor (280 nm)0.254
Extinction coefficient (cm -1 M -1)73000
Excitation (nm)491
Emission (nm)516
Quantum yield0.92
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


CAS
3326-32-7
Molecular weight
389.38
Correction Factor (280 nm)
0.254
Extinction coefficient (cm -1 M -1)
73000
Excitation (nm)
491
Emission (nm)
516
Quantum yield
0.92
5-FITC and 6-FITC have very similar absorption and fluorescence spectra. However, the isomers may differ in their binding and reactivities to proteins, and the conjugates may migrate differently in electrophoretic gels. Thus, we offer highly purified single isomers. Cautions must be exercised for the storage of FITC conjugates.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM256.819 µL1.284 mL2.568 mL12.841 mL25.682 mL
5 mM51.364 µL256.819 µL513.637 µL2.568 mL5.136 mL
10 mM25.682 µL128.409 µL256.819 µL1.284 mL2.568 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (280 nm)0.254
Extinction coefficient (cm -1 M -1)73000
Excitation (nm)491
Emission (nm)516
Quantum yield0.92

Citations


View all 3 citations: Citation Explorer
Determination of sodium benzoate in food products by fluorescence polarization immunoassay
Authors: Ren, Linlin and Meng, Meng and Wang, Peng and Xu, Zhihuan and Eremin, Sergei A and Zhao, Junhong and Yin, Yongmei and Xi, Rimo
Journal: Talanta (2014): 136--143
Complexes containing cationic and anionic pH-sensitive liposomes: comparative study of factors influencing plasmid DNA gene delivery to tumors
Authors: Chen, Yan and Sun, Ji and Lu, Ying and Tao, Chun and Huang, Jingbin and Zhang, He and Yu, Yuan and Zou, Hao and Gao, Jing and Zhong, Yanqiang
Journal: International journal of nanomedicine (2013): 1573
Swimming behavior of the monotrichous bacterium Pseudomonas fluorescens SBW25
Authors: Ping, Liyan and Birkenbeil, Jan and Monajembashi, Shamci
Journal: FEMS microbiology ecology (2013): 36--44

References


View all 103 references: Citation Explorer
Isothiocyanato Boron Dipyrromethenes - The First BODIPY Analogues of Fluorescein Isothiocyanate (FITC)
Authors: Malatesti N, Hudson R, Smith K, Savoie H, Rix K, Welham K, Boyle RW.
Journal: Photochem Photobiol. (2006)
Detection of FITC-cortisol via modulated supraparticle lighthouses
Authors: Petkus MM, McLauchlin M, Vuppu AK, Rios L, Garcia AA, Hayes MA.
Journal: Anal Chem (2006): 1405
Clathrin-mediated endocytosis of FITC-albumin in alveolar type II epithelial cell line RLE-6TN
Authors: Yumoto R, Nishikawa H, Okamoto M, Katayama H, Nagai J, Takano M.
Journal: Am J Physiol Lung Cell Mol Physiol (2006): L946
Fluorescein isothiocyanate labelled bovine serum albumin (FITC-BSA) as a model protein drug: opportunities and drawbacks
Authors: Wischke C, Borchert HH.
Journal: Pharmazie (2006): 770
Increased FITC fluorescence on LPS stimulated neutrophils cultured in whole blood
Authors: Wilkins RC, Bellier PV, Kutzner BC, McNamee JP.
Journal: Cell Biol Int (2006): 394
Effects of pretreatment of needle puncture and sandpaper abrasion on the in vitro skin permeation of fluorescein isothiocyanate (FITC)-dextran
Authors: Wu XM, Todo H, Sugibayashi K.
Journal: Int J Pharm (2006): 102
Uptake of dextran-FITC by epithelial cells of the chorioallantoic placentome and the omphalopleure of the placentotrophic lizard, Pseudemoia entrecasteauxii
Authors: Stewart JR, Thompson MB, Attaway MB, Herbert JF, Murphy CR.
Journal: J Exp Zoolog A Comp Exp Biol (2006): 883
Intracellular FITC-derivatization with PEG
Authors: Chen F, Zhi D, Xia G.
Journal: Electrophoresis (2005): 4204
Potential determination of aminated pesticides and metabolites by cyclodextrin capillary electrophoresis-laser-induced fluorescence using FITC as labelling
Authors: Navarrete-Casas M, Segura-Carretero A, Cruces-Blanco C, Fern and ez-Gutierrez A., undefined
Journal: Pest Manag Sci (2005): 197
Preparation and characterization of a directly labeled mouse anti-human CD14 monoclonal antibody ZCH-2F9-FITC.
Authors: Ning BT, Tang YM, Shen HQ, Yang SL, Chen YH, Song H, Shi SW, Qian BQ, Xu WQ.
Journal: Zhejiang Da Xue Xue Bao Yi Xue Ban (2005): 167