5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7*

5-FITC

Fluorescein-5-isothiocyanate; CAS 3326-32-7

5-FITC and 6-FITC have very similar absorption and fluorescence spectra. However, the isomers may differ in their binding and reactivities to proteins, and the conjugates may migrate differently in electrophoretic gels. Thus, we offer highly purified single isomers. Cautions must be exercised for the storage of FITC conjugates.

Datasheet

Catalog	Size	Price
120	100 mg	Price
121	1 g	Price
122	10 g	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	256.819 µL	1.284 mL	2.568 mL	12.841 mL	25.682 mL
5 mM	51.364 µL	256.819 µL	513.637 µL	2.568 mL	5.136 mL
10 mM	25.682 µL	128.409 µL	256.819 µL	1.284 mL	2.568 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	389.38 g/mol	=	mL	x	mM	=	mmol

Citations

View all 6 citations: Citation Explorer

Development of recombinant anti-human CD antibodies for diabetes and cancer monitoring using flow cytometry

Authors:

Ziyi, YANG and Xinxin, XU and Chuanlai, XU and others,

Journal:

Chinese Journal of Analytical Chemistry (2025): 100558

All-inorganic Polyoxometalates Act as Superchaotropic Membrane Carriers

Authors:

Barba-Bon, Andrea and Gumerova, Nadiia I and Tanuhadi, Elias and Ashjari, Maryam and Chen, Yao and Rompel, Annette and Nau, Werner M

Journal:

Advanced Materials (2023): 2309219

Determination of Nicotinic Acid in Food and Pharmaceuticals by a Simple and Rapid Fluorescence Polarization Immunoassay (FPIA)

Authors:

Qiu, Peng and Song, Zhaorui

Journal:

Analytical Letters (2023): 1--11

Determination of sodium benzoate in food products by fluorescence polarization immunoassay

Authors:

Ren, Linlin and Meng, Meng and Wang, Peng and Xu, Zhihuan and Eremin, Sergei A and Zhao, Junhong and Yin, Yongmei and Xi, Rimo

Journal:

Talanta (2014): 136--143

Complexes containing cationic and anionic pH-sensitive liposomes: comparative study of factors influencing plasmid DNA gene delivery to tumors

Authors:

Chen, Yan and Sun, Ji and Lu, Ying and Tao, Chun and Huang, Jingbin and Zhang, He and Yu, Yuan and Zou, Hao and Gao, Jing and Zhong, Yanqiang

Journal:

International journal of nanomedicine (2013): 1573

References

View all 103 references: Citation Explorer

Isothiocyanato Boron Dipyrromethenes - The First BODIPY Analogues of Fluorescein Isothiocyanate (FITC)

Authors:

Malatesti N, Hudson R, Smith K, Savoie H, Rix K, Welham K, Boyle RW.

Journal:

Photochem Photobiol. (2006)

Detection of FITC-cortisol via modulated supraparticle lighthouses

Authors:

Petkus MM, McLauchlin M, Vuppu AK, Rios L, Garcia AA, Hayes MA.

Journal:

Anal Chem (2006): 1405

Clathrin-mediated endocytosis of FITC-albumin in alveolar type II epithelial cell line RLE-6TN

Authors:

Yumoto R, Nishikawa H, Okamoto M, Katayama H, Nagai J, Takano M.

Journal:

Am J Physiol Lung Cell Mol Physiol (2006): L946

Fluorescein isothiocyanate labelled bovine serum albumin (FITC-BSA) as a model protein drug: opportunities and drawbacks

Authors:

Wischke C, Borchert HH.

Journal:

Pharmazie (2006): 770

Increased FITC fluorescence on LPS stimulated neutrophils cultured in whole blood

Authors:

Wilkins RC, Bellier PV, Kutzner BC, McNamee JP.

Journal:

Cell Biol Int (2006): 394

Physical properties

Molecular weight	389.38
Solvent	DMSO

Spectral properties

Absorbance (nm)	488
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.92

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure
CAS	3326-32-7

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