5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7*
Ordering information
| Price | |
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 389.38 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (280 nm) | 0.35 |
| Extinction coefficient (cm -1 M -1) | 73000 |
| Excitation (nm) | 491 |
| Emission (nm) | 516 |
| Quantum yield | 0.92 |
Storage, safety and handling
| Certificate of Origin | Download PDF |
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
See also: Amine Reactive Dyes and Probes for Conjugation, Bioconjugation, Chemical Reagents, Classic Dyes, Dyes by Functional Group, Förster Resonance Energy Transfer (FRET), Enzymes, Kinases, Fluoresceins
CAS 3326-32-7 | Molecular weight 389.38 | Correction Factor (280 nm) 0.35 | Extinction coefficient (cm -1 M -1) 73000 | Excitation (nm) 491 | Emission (nm) 516 | Quantum yield 0.92 |
5-FITC and 6-FITC have very similar absorption and fluorescence spectra. However, the isomers may differ in their binding and reactivities to proteins, and the conjugates may migrate differently in electrophoretic gels. Thus, we offer highly purified single isomers. Cautions must be exercised for the storage of FITC conjugates.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 256.819 µL | 1.284 mL | 2.568 mL | 12.841 mL | 25.682 mL |
| 5 mM | 51.364 µL | 256.819 µL | 513.637 µL | 2.568 mL | 5.136 mL |
| 10 mM | 25.682 µL | 128.409 µL | 256.819 µL | 1.284 mL | 2.568 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (280 nm) | 0.35 |
| Extinction coefficient (cm -1 M -1) | 73000 |
| Excitation (nm) | 491 |
| Emission (nm) | 516 |
| Quantum yield | 0.92 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| 5-FAM [5-Carboxyfluorescein] *CAS 76823-03-5* | 493 | 517 | 83000 | 0.178 |
| 5-SFX [5-FAM-X, SE] *CAS 148356-00-7* | 493 | 517 | 83000 | 0.178 |
| 5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5* | 498 | 517 | 800001 | 0.35 |
| 5-IAF [5-Iodoacetamidofluorescein] *CAS 63368-54-7* | 498 | 517 | 800001 | 0.35 |
| 5-CR110 [5-Carboxyrhodamine 110] *Single isomer* | 499 | 525 | 84000 | 0.091 |
| 5-CR6G [5-Carboxyrhodamine 6G] *Single isomer* | 522 | 546 | 94000 | 0.214 |
| 5-TAMRA [5-Carboxytetramethylrhodamine] *CAS 91809-66-4* | 552 | 578 | 90000 | 0.178 |
| 5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3* | 578 | 604 | 82000 | 0.168 |
| 5-TRITC [Tetramethylrhodamine-5-isothiocyanate] *CAS 80724-19-2* | 544 | 570 | 100000 | 0.34 |
Show More (3) | ||||
Images
Figure 1. Chemical structure for 5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7*
Citations
View all 4 citations: Citation Explorer
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Authors: Qiu, Peng and Song, Zhaorui
Journal: Analytical Letters (2023): 1--11
Authors: Qiu, Peng and Song, Zhaorui
Journal: Analytical Letters (2023): 1--11
Determination of sodium benzoate in food products by fluorescence polarization immunoassay
Authors: Ren, Linlin and Meng, Meng and Wang, Peng and Xu, Zhihuan and Eremin, Sergei A and Zhao, Junhong and Yin, Yongmei and Xi, Rimo
Journal: Talanta (2014): 136--143
Authors: Ren, Linlin and Meng, Meng and Wang, Peng and Xu, Zhihuan and Eremin, Sergei A and Zhao, Junhong and Yin, Yongmei and Xi, Rimo
Journal: Talanta (2014): 136--143
Complexes containing cationic and anionic pH-sensitive liposomes: comparative study of factors influencing plasmid DNA gene delivery to tumors
Authors: Chen, Yan and Sun, Ji and Lu, Ying and Tao, Chun and Huang, Jingbin and Zhang, He and Yu, Yuan and Zou, Hao and Gao, Jing and Zhong, Yanqiang
Journal: International journal of nanomedicine (2013): 1573
Authors: Chen, Yan and Sun, Ji and Lu, Ying and Tao, Chun and Huang, Jingbin and Zhang, He and Yu, Yuan and Zou, Hao and Gao, Jing and Zhong, Yanqiang
Journal: International journal of nanomedicine (2013): 1573
Swimming behavior of the monotrichous bacterium Pseudomonas fluorescens SBW25
Authors: Ping, Liyan and Birkenbeil, Jan and Monajembashi, Shamci
Journal: FEMS microbiology ecology (2013): 36--44
Authors: Ping, Liyan and Birkenbeil, Jan and Monajembashi, Shamci
Journal: FEMS microbiology ecology (2013): 36--44
References
View all 103 references: Citation Explorer
Isothiocyanato Boron Dipyrromethenes - The First BODIPY Analogues of Fluorescein Isothiocyanate (FITC)
Authors: Malatesti N, Hudson R, Smith K, Savoie H, Rix K, Welham K, Boyle RW.
Journal: Photochem Photobiol. (2006)
Authors: Malatesti N, Hudson R, Smith K, Savoie H, Rix K, Welham K, Boyle RW.
Journal: Photochem Photobiol. (2006)
Detection of FITC-cortisol via modulated supraparticle lighthouses
Authors: Petkus MM, McLauchlin M, Vuppu AK, Rios L, Garcia AA, Hayes MA.
Journal: Anal Chem (2006): 1405
Authors: Petkus MM, McLauchlin M, Vuppu AK, Rios L, Garcia AA, Hayes MA.
Journal: Anal Chem (2006): 1405
Clathrin-mediated endocytosis of FITC-albumin in alveolar type II epithelial cell line RLE-6TN
Authors: Yumoto R, Nishikawa H, Okamoto M, Katayama H, Nagai J, Takano M.
Journal: Am J Physiol Lung Cell Mol Physiol (2006): L946
Authors: Yumoto R, Nishikawa H, Okamoto M, Katayama H, Nagai J, Takano M.
Journal: Am J Physiol Lung Cell Mol Physiol (2006): L946
Fluorescein isothiocyanate labelled bovine serum albumin (FITC-BSA) as a model protein drug: opportunities and drawbacks
Authors: Wischke C, Borchert HH.
Journal: Pharmazie (2006): 770
Authors: Wischke C, Borchert HH.
Journal: Pharmazie (2006): 770
Increased FITC fluorescence on LPS stimulated neutrophils cultured in whole blood
Authors: Wilkins RC, Bellier PV, Kutzner BC, McNamee JP.
Journal: Cell Biol Int (2006): 394
Authors: Wilkins RC, Bellier PV, Kutzner BC, McNamee JP.
Journal: Cell Biol Int (2006): 394
Effects of pretreatment of needle puncture and sandpaper abrasion on the in vitro skin permeation of fluorescein isothiocyanate (FITC)-dextran
Authors: Wu XM, Todo H, Sugibayashi K.
Journal: Int J Pharm (2006): 102
Authors: Wu XM, Todo H, Sugibayashi K.
Journal: Int J Pharm (2006): 102
Uptake of dextran-FITC by epithelial cells of the chorioallantoic placentome and the omphalopleure of the placentotrophic lizard, Pseudemoia entrecasteauxii
Authors: Stewart JR, Thompson MB, Attaway MB, Herbert JF, Murphy CR.
Journal: J Exp Zoolog A Comp Exp Biol (2006): 883
Authors: Stewart JR, Thompson MB, Attaway MB, Herbert JF, Murphy CR.
Journal: J Exp Zoolog A Comp Exp Biol (2006): 883
Intracellular FITC-derivatization with PEG
Authors: Chen F, Zhi D, Xia G.
Journal: Electrophoresis (2005): 4204
Authors: Chen F, Zhi D, Xia G.
Journal: Electrophoresis (2005): 4204
Potential determination of aminated pesticides and metabolites by cyclodextrin capillary electrophoresis-laser-induced fluorescence using FITC as labelling
Authors: Navarrete-Casas M, Segura-Carretero A, Cruces-Blanco C, Fern and ez-Gutierrez A., undefined
Journal: Pest Manag Sci (2005): 197
Authors: Navarrete-Casas M, Segura-Carretero A, Cruces-Blanco C, Fern and ez-Gutierrez A., undefined
Journal: Pest Manag Sci (2005): 197
Preparation and characterization of a directly labeled mouse anti-human CD14 monoclonal antibody ZCH-2F9-FITC.
Authors: Ning BT, Tang YM, Shen HQ, Yang SL, Chen YH, Song H, Shi SW, Qian BQ, Xu WQ.
Journal: Zhejiang Da Xue Xue Bao Yi Xue Ban (2005): 167
Authors: Ning BT, Tang YM, Shen HQ, Yang SL, Chen YH, Song H, Shi SW, Qian BQ, Xu WQ.
Journal: Zhejiang Da Xue Xue Bao Yi Xue Ban (2005): 167
Application notes
Abbreviation of Common Chemical Compounds Related to Peptides
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis