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5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7*

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Physical properties
Molecular weight389.38
Spectral properties
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)73000
Excitation (nm)491
Emission (nm)516
Quantum yield0.92
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
5-FITC and 6-FITC have very similar absorption and fluorescence spectra. However, the isomers may differ in their binding and reactivities to proteins, and the conjugates may migrate differently in electrophoretic gels. Thus, we offer highly purified single isomers. Cautions must be exercised for the storage of FITC conjugates.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM256.819 µL1.284 mL2.568 mL12.841 mL25.682 mL
5 mM51.364 µL256.819 µL513.637 µL2.568 mL5.136 mL
10 mM25.682 µL128.409 µL256.819 µL1.284 mL2.568 mL

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Spectral properties

Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)73000
Excitation (nm)491
Emission (nm)516
Quantum yield0.92



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View all 103 references: Citation Explorer
Isothiocyanato Boron Dipyrromethenes - The First BODIPY Analogues of Fluorescein Isothiocyanate (FITC)
Authors: Malatesti N, Hudson R, Smith K, Savoie H, Rix K, Welham K, Boyle RW.
Journal: Photochem Photobiol. (2006)
Detection of FITC-cortisol via modulated supraparticle lighthouses
Authors: Petkus MM, McLauchlin M, Vuppu AK, Rios L, Garcia AA, Hayes MA.
Journal: Anal Chem (2006): 1405
Clathrin-mediated endocytosis of FITC-albumin in alveolar type II epithelial cell line RLE-6TN
Authors: Yumoto R, Nishikawa H, Okamoto M, Katayama H, Nagai J, Takano M.
Journal: Am J Physiol Lung Cell Mol Physiol (2006): L946
Fluorescein isothiocyanate labelled bovine serum albumin (FITC-BSA) as a model protein drug: opportunities and drawbacks
Authors: Wischke C, Borchert HH.
Journal: Pharmazie (2006): 770
Increased FITC fluorescence on LPS stimulated neutrophils cultured in whole blood
Authors: Wilkins RC, Bellier PV, Kutzner BC, McNamee JP.
Journal: Cell Biol Int (2006): 394
Effects of pretreatment of needle puncture and sandpaper abrasion on the in vitro skin permeation of fluorescein isothiocyanate (FITC)-dextran
Authors: Wu XM, Todo H, Sugibayashi K.
Journal: Int J Pharm (2006): 102
Uptake of dextran-FITC by epithelial cells of the chorioallantoic placentome and the omphalopleure of the placentotrophic lizard, Pseudemoia entrecasteauxii
Authors: Stewart JR, Thompson MB, Attaway MB, Herbert JF, Murphy CR.
Journal: J Exp Zoolog A Comp Exp Biol (2006): 883
Potential determination of aminated pesticides and metabolites by cyclodextrin capillary electrophoresis-laser-induced fluorescence using FITC as labelling
Authors: Navarrete-Casas M, Segura-Carretero A, Cruces-Blanco C, Fern and ez-Gutierrez A., undefined
Journal: Pest Manag Sci (2005): 197
Preparation of a gradient biotinylated polyethylene surface to bind streptavidin-FITC
Authors: Kim MS, Seo KS, Khang G, Lee HB.
Journal: Bioconjug Chem (2005): 245
FITC-induced murine pulmonary inflammation: CC10 up-regulation and concurrent Shh expression
Authors: Fisher CE, Ahmad SA, Fitch PM, Lamb JR, Howie SE.
Journal: Cell Biol Int (2005): 868