Calculator
5-IAF [5-Iodoacetamidofluorescein] *CAS 63368-54-7*
5-IAF is one of the most popular green fluorescent dyes for thiol modifications of proteins along with fluorescein-5-maleimide. The resulted bioconjugates are quite stable.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 5-IAF [5-Iodoacetamidofluorescein] *CAS 63368-54-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 194.081 µL | 970.403 µL | 1.941 mL | 9.704 mL | 19.408 mL |
| 5 mM | 38.816 µL | 194.081 µL | 388.161 µL | 1.941 mL | 3.882 mL |
| 10 mM | 19.408 µL | 97.04 µL | 194.081 µL | 970.403 µL | 1.941 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| 5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5* | 498 | 517 | 800001 | 0.35 |
| 5-FAM [5-Carboxyfluorescein] *CAS 76823-03-5* | 493 | 517 | 83000 | 0.178 |
| 5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7* | 491 | 516 | 73000 | 0.35 |
| 5-SFX [5-FAM-X, SE] *CAS 148356-00-7* | 493 | 517 | 83000 | 0.178 |
References
View all 10 references: Citation Explorer
Quantitation of reduced disulfide groups in monoclonal antibodies using 5-iodoacetamidofluorescein: a novel size exclusion-HPLC technique
Authors: Muddukrishna SN, Chen A, Qi P, Smolenski MA.
Journal: Appl Radiat Isot (1995): 1015
Authors: Muddukrishna SN, Chen A, Qi P, Smolenski MA.
Journal: Appl Radiat Isot (1995): 1015
Na,K-ATPase labelled with 5-iodoacetamidofluorescein: E2-E1 conformational transition induced by different nucleotides
Authors: Lopina OD, Pindel EV, Boldyrev AA.
Journal: FEBS Lett (1990): 75
Authors: Lopina OD, Pindel EV, Boldyrev AA.
Journal: FEBS Lett (1990): 75
Studies on conformational changes in Na,K-ATPase labeled with 5-iodoacetamidofluorescein
Authors: Steinberg M, Karlish SJ.
Journal: J Biol Chem (1989): 2726
Authors: Steinberg M, Karlish SJ.
Journal: J Biol Chem (1989): 2726
Identification of the 5-iodoacetamidofluorescein reporter site on the Na,K-ATPase
Authors: Tyson PA, Steinberg M, Wallick ET, Kirley TL.
Journal: J Biol Chem (1989): 726
Authors: Tyson PA, Steinberg M, Wallick ET, Kirley TL.
Journal: J Biol Chem (1989): 726
Distances between 5-iodoacetamidofluorescein and the ATP and ouabain sites of (Na,K)-ATPase determined by fluorescence energy transfer
Authors: Fortes PA, Aguilar R.
Journal: Prog Clin Biol Res (1988): 197
Authors: Fortes PA, Aguilar R.
Journal: Prog Clin Biol Res (1988): 197
Page updated on May 19, 2025
Safety data sheet