5-IAF [5-Iodoacetamidofluorescein] CAS 63368-54-7

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Physical properties

Molecular weight	515.25
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

63368-54-7

Molecular weight

515.25

Absorbance (nm)

487

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.35

Extinction coefficient (cm ^-1 M ^-1)

80000¹

Excitation (nm)

498

Emission (nm)

517

Quantum yield

0.7900¹, 0.95²

5-IAF is one of the most popular green fluorescent dyes for thiol modifications of proteins along with fluorescein-5-maleimide. The resulted bioconjugates are quite stable.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-IAF [5-Iodoacetamidofluorescein] *CAS 63368-54-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	194.081 µL	970.403 µL	1.941 mL	9.704 mL	19.408 mL
5 mM	38.816 µL	194.081 µL	388.161 µL	1.941 mL	3.882 mL
10 mM	19.408 µL	97.04 µL	194.081 µL	970.403 µL	1.941 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
5-FAM [5-Carboxyfluorescein] CAS 76823-03-5	493	517	83000	0.178
5-SFX [5-FAM-X, SE] CAS 148356-00-7	493	517	83000	0.178
5-FITC [Fluorescein-5-isothiocyanate] CAS 3326-32-7	491	516	73000	0.35
5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] CAS 51306-35-5	498	517	80000¹	0.35
5-CR110 [5-Carboxyrhodamine 110] Single isomer	499	525	84000	0.091
5-CR6G [5-Carboxyrhodamine 6G] Single isomer	522	546	94000	0.214
5-TAMRA [5-Carboxytetramethylrhodamine] CAS 91809-66-4	552	578	90000	0.178
5-ROX [5-Carboxy-X-rhodamine] CAS 216699-35-3	578	604	82000	0.168
5-TRITC [Tetramethylrhodamine-5-isothiocyanate] CAS 80724-19-2	544	570	100000	0.34
DiBAC4(5) [Bis-(1,3-dibutylbarbituric acid)pentamethine oxonol]	591	615	-	-
DiSBAC2(5) [Bis-(1,3-diethylthiobarbituric acid)pentamethine oxonol]	636	655	-	-
Show More (2)

Images

Figure 1. Chemical structure for 5-IAF [5-Iodoacetamidofluorescein] *CAS 63368-54-7*

References

View all 10 references: Citation Explorer

Quantitation of reduced disulfide groups in monoclonal antibodies using 5-iodoacetamidofluorescein: a novel size exclusion-HPLC technique
Authors: Muddukrishna SN, Chen A, Qi P, Smolenski MA.
Journal: Appl Radiat Isot (1995): 1015

Na,K-ATPase labelled with 5-iodoacetamidofluorescein: E2-E1 conformational transition induced by different nucleotides
Authors: Lopina OD, Pindel EV, Boldyrev AA.
Journal: FEBS Lett (1990): 75

Identification of the 5-iodoacetamidofluorescein reporter site on the Na,K-ATPase
Authors: Tyson PA, Steinberg M, Wallick ET, Kirley TL.
Journal: J Biol Chem (1989): 726

Studies on conformational changes in Na,K-ATPase labeled with 5-iodoacetamidofluorescein
Authors: Steinberg M, Karlish SJ.
Journal: J Biol Chem (1989): 2726

Distances between 5-iodoacetamidofluorescein and the ATP and ouabain sites of (Na,K)-ATPase determined by fluorescence energy transfer
Authors: Fortes PA, Aguilar R.
Journal: Prog Clin Biol Res (1988): 197

Ligand binding to (Na,K)-ATPase labeled with 5-iodoacetamidofluorescein
Authors: Kapakos JG, Steinberg M.
Journal: J Biol Chem (1986): 2084

Steady-state fluorescence emission from the fluorescent probe, 5-iodoacetamidofluorescein, bound to hemoglobin
Authors: Hirsch RE, Zukin RS, Nagel RL.
Journal: Biochem Biophys Res Commun (1986): 489

5-Iodoacetamidofluorescein-labeled (Na,K)-ATPase. Steady-state fluorescence during turnover
Authors: Kapakos JG, Steinberg M.
Journal: J Biol Chem (1986): 2090

Fluorescent labeling of (Na+ + K+)-ATPase by 5-iodoacetamidofluorescein
Authors: Kapakos JG, Steinberg M.
Journal: Biochim Biophys Acta (1982): 493

Preparation and characterization of a new molecular cytochemical probe: 5-iodoacetamidofluorescein-labeled actin
Authors: Wang YL, Taylor DL.
Journal: J Histochem Cytochem (1980): 1198