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5-IAF [5-Iodoacetamidofluorescein] *CAS 63368-54-7*

Chemical structure for 5-IAF [5-Iodoacetamidofluorescein] *CAS 63368-54-7*
Chemical structure for 5-IAF [5-Iodoacetamidofluorescein] *CAS 63368-54-7*
Ordering information
Price ()
Catalog Number222
Unit Size
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Physical properties
Molecular weight515.25
SolventDMSO
Spectral properties
Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.275
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


CAS
63368-54-7
Molecular weight
515.25
Absorbance (nm)
487
Correction Factor (260 nm)
0.32
Correction Factor (280 nm)
0.275
Extinction coefficient (cm -1 M -1)
800001
Excitation (nm)
498
Emission (nm)
517
Quantum yield
0.79001, 0.952
5-IAF is one of the most popular green fluorescent dyes for thiol modifications of proteins along with fluorescein-5-maleimide. The resulted bioconjugates are quite stable.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-IAF [5-Iodoacetamidofluorescein] *CAS 63368-54-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM194.081 µL970.403 µL1.941 mL9.704 mL19.408 mL
5 mM38.816 µL194.081 µL388.161 µL1.941 mL3.882 mL
10 mM19.408 µL97.04 µL194.081 µL970.403 µL1.941 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.275
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952

References


View all 10 references: Citation Explorer
Quantitation of reduced disulfide groups in monoclonal antibodies using 5-iodoacetamidofluorescein: a novel size exclusion-HPLC technique
Authors: Muddukrishna SN, Chen A, Qi P, Smolenski MA.
Journal: Appl Radiat Isot (1995): 1015
Na,K-ATPase labelled with 5-iodoacetamidofluorescein: E2-E1 conformational transition induced by different nucleotides
Authors: Lopina OD, Pindel EV, Boldyrev AA.
Journal: FEBS Lett (1990): 75
Identification of the 5-iodoacetamidofluorescein reporter site on the Na,K-ATPase
Authors: Tyson PA, Steinberg M, Wallick ET, Kirley TL.
Journal: J Biol Chem (1989): 726
Studies on conformational changes in Na,K-ATPase labeled with 5-iodoacetamidofluorescein
Authors: Steinberg M, Karlish SJ.
Journal: J Biol Chem (1989): 2726
Distances between 5-iodoacetamidofluorescein and the ATP and ouabain sites of (Na,K)-ATPase determined by fluorescence energy transfer
Authors: Fortes PA, Aguilar R.
Journal: Prog Clin Biol Res (1988): 197
Ligand binding to (Na,K)-ATPase labeled with 5-iodoacetamidofluorescein
Authors: Kapakos JG, Steinberg M.
Journal: J Biol Chem (1986): 2084
Steady-state fluorescence emission from the fluorescent probe, 5-iodoacetamidofluorescein, bound to hemoglobin
Authors: Hirsch RE, Zukin RS, Nagel RL.
Journal: Biochem Biophys Res Commun (1986): 489
5-Iodoacetamidofluorescein-labeled (Na,K)-ATPase. Steady-state fluorescence during turnover
Authors: Kapakos JG, Steinberg M.
Journal: J Biol Chem (1986): 2090
Fluorescent labeling of (Na+ + K+)-ATPase by 5-iodoacetamidofluorescein
Authors: Kapakos JG, Steinberg M.
Journal: Biochim Biophys Acta (1982): 493
Preparation and characterization of a new molecular cytochemical probe: 5-iodoacetamidofluorescein-labeled actin
Authors: Wang YL, Taylor DL.
Journal: J Histochem Cytochem (1980): 1198