5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5*

5-DTAF

5-(4,6-Dichlorotriazinyl)aminofluorescein; CAS 51306-35-5

The 5-isomer of fluorescein dichlorotriazine (5-DTAF) is highly reactive with proteins. Unlike other reactive fluoresceins, 5-DTAF not only reacts with amino groups, but also reacts with thiol groups and even directly reacts with hydroxy groups such as polysaccharides and other alcohols in aqueous solution at pH above 9. Due to its high reactivity, 5-DTAF is also used to label carbohydrates.

Protocol

COA

Catalog	Size	Price	Quantity
200	25 mg	Price
201	10 g	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	201.906 µL	1.01 mL	2.019 mL	10.095 mL	20.191 mL
5 mM	40.381 µL	201.906 µL	403.812 µL	2.019 mL	4.038 mL
10 mM	20.191 µL	100.953 µL	201.906 µL	1.01 mL	2.019 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	495.28 g/mol	=	mL	x	mM	=	mmol

Citations

View all 4 citations: Citation Explorer

An inulin-based glycovesicle for pathogen-targeted drug delivery to ameliorate salmonellosis

Authors:

Xu, Yujie and Niu, Congmin and Liang, Shuyi and Guo, Jiayi and Li, Kaiming and Zhang, Jiarui and Li, Jingyuan and Jin, Yaju and Bai, Jingkun and Dai, Jiangkun and others,

Journal:

International Journal of Biological Macromolecules (2024): 131656

Cellulose Dissolved in Ionic Liquids for Modification of the Shape of Keratin Fibers

Authors:

Liu, Yuwei and Jing, Su and Carvalho, Diana and Fu, Jiajia and Martins, Madalena and Cavaco-Paulo, Artur

Journal:

ACS Sustainable Chemistry \& Engineering (2021): 4102--4110

3D Confocal Laser Scanning Microscopy for Quantification of the Phase Behaviour in Agarose-MCC co-gels in Comparison to the Rheological Blending-law Analysis

Authors:

Mhaske, Pranita and Farahnaky, Asgar and Kasapis, Stefan

Journal:

Food Biophysics (2020): 1--8

Trophic strategies of freshwater nanoflagellates under variable run-off scenarios

Authors:

Symiakaki, Katerina and Berger, Stella A and {\'A}greda-L{\'o}pez, Gabriela and Butty{\'a}n, Bence and Gerg{\'a}cz, Bence and Langenheder, Silke and Nejstgaard, Jens C

Journal:

Limnology and Oceanography

References

View all 30 references: Citation Explorer

Fluorescently labeled collagen binding proteins allow specific visualization of collagen in tissues and live cell culture

Authors:

Krahn KN, Bouten CV, van Tuijl S, van Z and voort MA, Merkx M.

Journal:

Anal Biochem (2006): 177

Mitomycin C alters corneal stromal wound healing and corneal haze in rabbits after argon-fluoride excimer laser photorefractive keratectomy

Authors:

Lai YH, Wang HZ, Lin CP, Chang SJ.

Journal:

J Ocul Pharmacol Ther (2004): 129

Fluorescent cellulose microfibrils as substrate for the detection of cellulase activity

Authors:

Helbert W, Chanzy H, Husum TL, Schulein M, Ernst S.

Journal:

Biomacromolecules (2003): 481

Microbial activity in soils following steam treatment

Authors:

Richardson RE, James CA, Bhupathiraju VK, Alvarez-Cohen L.

Journal:

Biodegradation (2002): 285

Effect of collagenase inhibitors on corneal haze after PRK

Authors:

Corbett MC, O'Brart DP, Patmore AL, Marshall J.

Journal:

Exp Eye Res (2001): 253

Physical properties

Molecular weight	495.28
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	51306-35-5

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