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5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5*

Chemical structure for 5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5*
Chemical structure for 5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5*
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Catalog Number200
Unit Size
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Physical properties
Molecular weight495.28
SolventDMSO
Spectral properties
Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.275
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


CAS
51306-35-5
Molecular weight
495.28
Absorbance (nm)
487
Correction Factor (260 nm)
0.32
Correction Factor (280 nm)
0.275
Extinction coefficient (cm -1 M -1)
800001
Excitation (nm)
498
Emission (nm)
517
Quantum yield
0.79001, 0.952
The 5-isomer of fluorescein dichlorotriazine (5-DTAF) is highly reactive with proteins. Unlike other reactive fluoresceins, 5-DTAF not only reacts with amino groups, but also reacts with thiol groups and even directly reacts with hydroxy groups such as polysaccharides and other alcohols in aqueous solution at pH above 9. Due to its high reactivity, 5-DTAF is also used to label carbohydrates.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM201.906 µL1.01 mL2.019 mL10.095 mL20.191 mL
5 mM40.381 µL201.906 µL403.812 µL2.019 mL4.038 mL
10 mM20.191 µL100.953 µL201.906 µL1.01 mL2.019 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.275
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952

Citations


View all 2 citations: Citation Explorer
Cellulose Dissolved in Ionic Liquids for Modification of the Shape of Keratin Fibers
Authors: Liu, Yuwei and Jing, Su and Carvalho, Diana and Fu, Jiajia and Martins, Madalena and Cavaco-Paulo, Artur
Journal: ACS Sustainable Chemistry \& Engineering (2021): 4102--4110

References


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