AAT Bioquest

5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5*

Product Image
Product Image
Gallery Image 1
Ordering information
Catalog Number
Unit Size
Add to cart
Additional ordering information
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight495.28
Spectral properties
Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


See also: Fluoresceins
Molecular weight
Absorbance (nm)
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
0.79001, 0.952
The 5-isomer of fluorescein dichlorotriazine (5-DTAF) is highly reactive with proteins. Unlike other reactive fluoresceins, 5-DTAF not only reacts with amino groups, but also reacts with thiol groups and even directly reacts with hydroxy groups such as polysaccharides and other alcohols in aqueous solution at pH above 9. Due to its high reactivity, 5-DTAF is also used to label carbohydrates.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM201.906 µL1.01 mL2.019 mL10.095 mL20.191 mL
5 mM40.381 µL201.906 µL403.812 µL2.019 mL4.038 mL
10 mM20.191 µL100.953 µL201.906 µL1.01 mL2.019 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles


Open in Advanced Spectrum Viewer

Spectral properties

Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952



View all 3 citations: Citation Explorer
An inulin-based glycovesicle for pathogen-targeted drug delivery to ameliorate salmonellosis
Authors: Xu, Yujie and Niu, Congmin and Liang, Shuyi and Guo, Jiayi and Li, Kaiming and Zhang, Jiarui and Li, Jingyuan and Jin, Yaju and Bai, Jingkun and Dai, Jiangkun and others,
Journal: International Journal of Biological Macromolecules (2024): 131656
Cellulose Dissolved in Ionic Liquids for Modification of the Shape of Keratin Fibers
Authors: Liu, Yuwei and Jing, Su and Carvalho, Diana and Fu, Jiajia and Martins, Madalena and Cavaco-Paulo, Artur
Journal: ACS Sustainable Chemistry \& Engineering (2021): 4102--4110


View all 30 references: Citation Explorer
Fluorescently labeled collagen binding proteins allow specific visualization of collagen in tissues and live cell culture
Authors: Krahn KN, Bouten CV, van Tuijl S, van Z and voort MA, Merkx M.
Journal: Anal Biochem (2006): 177
Mitomycin C alters corneal stromal wound healing and corneal haze in rabbits after argon-fluoride excimer laser photorefractive keratectomy
Authors: Lai YH, Wang HZ, Lin CP, Chang SJ.
Journal: J Ocul Pharmacol Ther (2004): 129
Fluorescent cellulose microfibrils as substrate for the detection of cellulase activity
Authors: Helbert W, Chanzy H, Husum TL, Schulein M, Ernst S.
Journal: Biomacromolecules (2003): 481
Microbial activity in soils following steam treatment
Authors: Richardson RE, James CA, Bhupathiraju VK, Alvarez-Cohen L.
Journal: Biodegradation (2002): 285
Effect of collagenase inhibitors on corneal haze after PRK
Authors: Corbett MC, O'Brart DP, Patmore AL, Marshall J.
Journal: Exp Eye Res (2001): 253
Glutamic acid decarboxylase-like immunoreactive axon terminals in synaptic contact with mesencephalic trigeminal nucleus neurons in the rat
Authors: Chen P, Li J, Mizuno N.
Journal: Neurosci Lett (2001): 167
Redistribution of plasma-membrane surface molecules during formation of the Leishmania amazonensis-containing parasitophorous vacuole
Authors: Henriques C, de Souza W.
Journal: Parasitol Res (2000): 215
Application of a tetrazolium dye as an indicator of viability in anaerobic bacteria
Authors: Bhupathiraju VK, Hern and ez M, L and fear D, Alvarez-Cohen L.
Journal: J Microbiol Methods (1999): 231
Leakage responses to L-NAME differ with the fluorescent dye used to label albumin
Authors: Rumbaut RE, Harris NR, Sial AJ, Huxley VH, Granger DN.
Journal: Am J Physiol (1999): H333
Collagen fiber sliding during ligament growth and contracture
Authors: Wood ML, Lester GE, Dahners LE.
Journal: J Orthop Res (1998): 438