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5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5*
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Application notes
The 5-isomer of fluorescein dichlorotriazine (5-DTAF) is highly reactive with proteins. Unlike other reactive fluoresceins, 5-DTAF not only reacts with amino groups, but also reacts with thiol groups and even directly reacts with hydroxy groups such as polysaccharides and other alcohols in aqueous solution at pH above 9. Due to its high reactivity, 5-DTAF is also used to label carbohydrates.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 201.906 µL | 1.01 mL | 2.019 mL | 10.095 mL | 20.191 mL |
| 5 mM | 40.381 µL | 201.906 µL | 403.812 µL | 2.019 mL | 4.038 mL |
| 10 mM | 20.191 µL | 100.953 µL | 201.906 µL | 1.01 mL | 2.019 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| 5-FAM [5-Carboxyfluorescein] *CAS 76823-03-5* | 493 | 517 | 83000 | 0.178 |
| 5-SFX [5-FAM-X, SE] *CAS 148356-00-7* | 493 | 517 | 83000 | 0.178 |
| 5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7* | 491 | 516 | 73000 | 0.35 |
| 6-DTAF [6-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 118357-32-7* | 498 | 517 | 800001 | 0.35 |
| 5-IAF [5-Iodoacetamidofluorescein] *CAS 63368-54-7* | 498 | 517 | 800001 | 0.35 |
| 5-CR110 [5-Carboxyrhodamine 110] *Single isomer* | 499 | 525 | 84000 | 0.091 |
| 5-CR6G [5-Carboxyrhodamine 6G] *Single isomer* | 522 | 546 | 94000 | 0.214 |
| 5-TAMRA [5-Carboxytetramethylrhodamine] *CAS 91809-66-4* | 552 | 578 | 90000 | 0.178 |
| 5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3* | 578 | 604 | 82000 | 0.168 |
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Citations
View all 3 citations: Citation Explorer
An inulin-based glycovesicle for pathogen-targeted drug delivery to ameliorate salmonellosis
Authors: Xu, Yujie and Niu, Congmin and Liang, Shuyi and Guo, Jiayi and Li, Kaiming and Zhang, Jiarui and Li, Jingyuan and Jin, Yaju and Bai, Jingkun and Dai, Jiangkun and others,
Journal: International Journal of Biological Macromolecules (2024): 131656
Authors: Xu, Yujie and Niu, Congmin and Liang, Shuyi and Guo, Jiayi and Li, Kaiming and Zhang, Jiarui and Li, Jingyuan and Jin, Yaju and Bai, Jingkun and Dai, Jiangkun and others,
Journal: International Journal of Biological Macromolecules (2024): 131656
Cellulose Dissolved in Ionic Liquids for Modification of the Shape of Keratin Fibers
Authors: Liu, Yuwei and Jing, Su and Carvalho, Diana and Fu, Jiajia and Martins, Madalena and Cavaco-Paulo, Artur
Journal: ACS Sustainable Chemistry \& Engineering (2021): 4102--4110
Authors: Liu, Yuwei and Jing, Su and Carvalho, Diana and Fu, Jiajia and Martins, Madalena and Cavaco-Paulo, Artur
Journal: ACS Sustainable Chemistry \& Engineering (2021): 4102--4110
3D Confocal Laser Scanning Microscopy for Quantification of the Phase Behaviour in Agarose-MCC co-gels in Comparison to the Rheological Blending-law Analysis
Authors: Mhaske, Pranita and Farahnaky, Asgar and Kasapis, Stefan
Journal: Food Biophysics (2020): 1--8
Authors: Mhaske, Pranita and Farahnaky, Asgar and Kasapis, Stefan
Journal: Food Biophysics (2020): 1--8
References
View all 30 references: Citation Explorer
Fluorescently labeled collagen binding proteins allow specific visualization of collagen in tissues and live cell culture
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Journal: Anal Biochem (2006): 177
Authors: Krahn KN, Bouten CV, van Tuijl S, van Z and voort MA, Merkx M.
Journal: Anal Biochem (2006): 177
Mitomycin C alters corneal stromal wound healing and corneal haze in rabbits after argon-fluoride excimer laser photorefractive keratectomy
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Journal: J Ocul Pharmacol Ther (2004): 129
Authors: Lai YH, Wang HZ, Lin CP, Chang SJ.
Journal: J Ocul Pharmacol Ther (2004): 129
Fluorescent cellulose microfibrils as substrate for the detection of cellulase activity
Authors: Helbert W, Chanzy H, Husum TL, Schulein M, Ernst S.
Journal: Biomacromolecules (2003): 481
Authors: Helbert W, Chanzy H, Husum TL, Schulein M, Ernst S.
Journal: Biomacromolecules (2003): 481
Microbial activity in soils following steam treatment
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Journal: Biodegradation (2002): 285
Authors: Richardson RE, James CA, Bhupathiraju VK, Alvarez-Cohen L.
Journal: Biodegradation (2002): 285
Effect of collagenase inhibitors on corneal haze after PRK
Authors: Corbett MC, O'Brart DP, Patmore AL, Marshall J.
Journal: Exp Eye Res (2001): 253
Authors: Corbett MC, O'Brart DP, Patmore AL, Marshall J.
Journal: Exp Eye Res (2001): 253