5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] CAS 51306-35-5

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Physical properties

Molecular weight	495.28
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	201.906 µL	1.01 mL	2.019 mL	10.095 mL	20.191 mL
5 mM	40.381 µL	201.906 µL	403.812 µL	2.019 mL	4.038 mL
10 mM	20.191 µL	100.953 µL	201.906 µL	1.01 mL	2.019 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
5-FAM [5-Carboxyfluorescein] CAS 76823-03-5	493	517	83000	0.178
5-SFX [5-FAM-X, SE] CAS 148356-00-7	493	517	83000	0.178
5-FITC [Fluorescein-5-isothiocyanate] CAS 3326-32-7	491	516	73000	0.35
6-DTAF [6-(4,6-Dichlorotriazinyl)aminofluorescein] CAS 118357-32-7	498	517	80000¹	0.35
5-IAF [5-Iodoacetamidofluorescein] CAS 63368-54-7	498	517	80000¹	0.35
5-CR110 [5-Carboxyrhodamine 110] Single isomer	499	525	84000	0.091
5-CR6G [5-Carboxyrhodamine 6G] Single isomer	522	546	94000	0.214
5-TAMRA [5-Carboxytetramethylrhodamine] CAS 91809-66-4	552	578	90000	0.178
5-ROX [5-Carboxy-X-rhodamine] CAS 216699-35-3	578	604	82000	0.168
5-TRITC [Tetramethylrhodamine-5-isothiocyanate] CAS 80724-19-2	544	570	100000	0.34
DiBAC4(5) [Bis-(1,3-dibutylbarbituric acid)pentamethine oxonol]	591	615	-	-
DiSBAC2(5) [Bis-(1,3-diethylthiobarbituric acid)pentamethine oxonol]	636	655	-	-
Show More (3)

Images

Figure 1. Chemical structure for 5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5*

Citations

View all 2 citations: Citation Explorer

Cellulose Dissolved in Ionic Liquids for Modification of the Shape of Keratin Fibers
Authors: Liu, Yuwei and Jing, Su and Carvalho, Diana and Fu, Jiajia and Martins, Madalena and Cavaco-Paulo, Artur
Journal: ACS Sustainable Chemistry \& Engineering (2021): 4102--4110

3D Confocal Laser Scanning Microscopy for Quantification of the Phase Behaviour in Agarose-MCC co-gels in Comparison to the Rheological Blending-law Analysis
Authors: Mhaske, Pranita and Farahnaky, Asgar and Kasapis, Stefan
Journal: Food Biophysics (2020): 1--8

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