Name: 6-DTAF [6-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 118357-32-7*
Brand: AAT Bioquest
SKU: 205
Price: 2286 USD
Availability: InStock

6-DTAF

6-(4,6-Dichlorotriazinyl)aminofluorescein; CAS 118357-32-7

Compared to 5-DTAF, the 6-isomer is more preferably used for labeling oligonucleotides and nucleic acids. Like 5-DTAF, 6-DTAF not only reacts with amino groups, but also reacts with thiol groups and even directly reacts with hydroxy groups such as polysaccharides and other alcohols in aqueous solution at pH above 9. Due to its high reactivity, 6-DTAF is also used to label carbohydrates.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
205	1 g	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-DTAF [6-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 118357-32-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	201.906 µL	1.01 mL	2.019 mL	10.095 mL	20.191 mL
5 mM	40.381 µL	201.906 µL	403.812 µL	2.019 mL	4.038 mL
10 mM	20.191 µL	100.953 µL	201.906 µL	1.01 mL	2.019 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	495.28 g/mol	=	mL	x	mM	=	mmol

References

View all 30 references: Citation Explorer

Fluorescently labeled collagen binding proteins allow specific visualization of collagen in tissues and live cell culture

Authors:

Krahn KN, Bouten CV, van Tuijl S, van Z and voort MA, Merkx M.

Journal:

Anal Biochem (2006): 177

Mitomycin C alters corneal stromal wound healing and corneal haze in rabbits after argon-fluoride excimer laser photorefractive keratectomy

Authors:

Lai YH, Wang HZ, Lin CP, Chang SJ.

Journal:

J Ocul Pharmacol Ther (2004): 129

Fluorescent cellulose microfibrils as substrate for the detection of cellulase activity

Authors:

Helbert W, Chanzy H, Husum TL, Schulein M, Ernst S.

Journal:

Biomacromolecules (2003): 481

Microbial activity in soils following steam treatment

Authors:

Richardson RE, James CA, Bhupathiraju VK, Alvarez-Cohen L.

Journal:

Biodegradation (2002): 285

Effect of collagenase inhibitors on corneal haze after PRK

Authors:

Corbett MC, O'Brart DP, Patmore AL, Marshall J.

Journal:

Exp Eye Res (2001): 253

Physical properties

Molecular weight	495.28
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	118357-32-7

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Page updated on October 12, 2025