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FITC-xtra
Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with FITC-xtra are far superior compared to the corresponding FITC conjugates. FITC-xtra conjugates are significantly brighter than FITC conjugates and are much more photostable. Additionally, the fluorescence of FITC-xtra is not affected by pH (4-10). This pH insensitivity is a major improvement over FITC, which emits its maximum fluorescence only at pH above 9. FITC-xtra has spectral properties almost identical FITC. In addition, FITC-xtra give much higher conjugation yield under mild conjugation conditions than FITC. Like 5-FITC, FITC-xtra antibody conjugates have excitation ideally suited to the 488 nm laser line, making them alternatives to the corresponding FITC-labeled antibody conjugates. Under the same conditions tested, FITC-xtra antibody conjugates give much higher signal/background ratios than the corresponding FITC-labeled conjugates.
HeLa cells were incubated with (Tubulin+) or without (Tubulin-) mouse anti-tubulin and biotin goat anti-mouse IgG followed by FITC-Xtra streptavidin conjugate (Green, Left) or FITC-streptavidin conjugate (Green, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17530).
HeLa cells were incubated with (Tubulin+) or without (Tubulin-) mouse anti-tubulin and biotin goat anti-mouse IgG followed by FITC-Xtra streptavidin conjugate (Green, Left) or FITC-streptavidin conjugate (Green, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17530).
protocol
Protocol
CatalogSize
Price
Quantity
1355 mg
Price
13625 mg
Price
 
Physical properties
Molecular weight620.52
SolventDMSO
Spectral properties
Correction factor (280 nm)0.16
Extinction coefficient (cm -1 M -1)
75000
1
Excitation (nm)498
Emission (nm)526
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Documents
Protocol
Safety Data Sheet (Catalog 135)
Safety Data Sheet (Catalog 136)
Certificate of Analysis
Brochure: Labeling Antibodies & Biopolymers
Brochure: Secondary Detection Probes & Kits
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Page updated on October 31, 2025