5-TRITC [Tetramethylrhodamine-5-isothiocyanate] *CAS 80724-19-2*
5-TRITC (also called G isomer) is a purified single isomer of the 5(6)-TRITC mixed isomers. This labeling reagent is predominantly used in labeling peptides and proteins. Cautions must be exercised for the storage of TRITC conjugates.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 5-TRITC [Tetramethylrhodamine-5-isothiocyanate] *CAS 80724-19-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 208.342 µL | 1.042 mL | 2.083 mL | 10.417 mL | 20.834 mL |
5 mM | 41.668 µL | 208.342 µL | 416.684 µL | 2.083 mL | 4.167 mL |
10 mM | 20.834 µL | 104.171 µL | 208.342 µL | 1.042 mL | 2.083 mL |
Molarity calculator
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Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
5-CR110 [5-Carboxyrhodamine 110] *Single isomer* | 499 | 525 | 84000 | 0.24 | 0.091 |
5-CR6G [5-Carboxyrhodamine 6G] *Single isomer* | 522 | 546 | 94000 | 0.24 | 0.214 |
5-TAMRA [5-Carboxytetramethylrhodamine] *CAS 91809-66-4* | 552 | 578 | 90000 | 0.32 | 0.178 |
5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3* | 578 | 604 | 82000 | - | 0.168 |
6-TRITC [Tetramethylrhodamine-6-isothiocyanate] *CAS 80724-20-5* | 544 | 570 | 100000 | 0.27 | 0.34 |
5-FAM [5-Carboxyfluorescein] *CAS 76823-03-5* | 493 | 517 | 83000 | 0.32 | 0.178 |
5-SFX [5-FAM-X, SE] *CAS 148356-00-7* | 493 | 517 | 83000 | 0.32 | 0.178 |
5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7* | 491 | 516 | 73000 | - | 0.35 |
5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5* | 498 | 517 | 800001 | 0.32 | 0.35 |
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References
View all 45 references: Citation Explorer
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Journal: Tissue Cell (2004): 189
Resonance energy transfer nanobiosensors based on affinity binding between apo-enzyme and its substrate
Authors: Chinnayelka S, McShane MJ.
Journal: Biomacromolecules (2004): 1657
Authors: Chinnayelka S, McShane MJ.
Journal: Biomacromolecules (2004): 1657
Nontypeable Haemophilus influenzae strain 2019 produces a biofilm containing N-acetylneuraminic acid that may mimic sialylated O-linked glycans
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Journal: Infect Immun (2004): 4249
Authors: Greiner LL, Watanabe H, Phillips NJ, Shao J, Morgan A, Zaleski A, Gibson BW, Apicella MA.
Journal: Infect Immun (2004): 4249
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