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5-TRITC [Tetramethylrhodamine-5-isothiocyanate] *CAS 80724-19-2*

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Physical properties
Molecular weight479.98
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.27
Correction Factor (280 nm)0.34
Extinction coefficient (cm -1 M -1)100000
Excitation (nm)544
Emission (nm)570
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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OverviewpdfSDSpdfProtocol


CAS
80724-19-2
Molecular weight
479.98
Correction Factor (260 nm)
0.27
Correction Factor (280 nm)
0.34
Extinction coefficient (cm -1 M -1)
100000
Excitation (nm)
544
Emission (nm)
570
5-TRITC (also called G isomer) is a purified single isomer of the 5(6)-TRITC mixed isomers. This labeling reagent is predominantly used in labeling peptides and proteins. Cautions must be exercised for the storage of TRITC conjugates.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-TRITC [Tetramethylrhodamine-5-isothiocyanate] *CAS 80724-19-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM208.342 µL1.042 mL2.083 mL10.417 mL20.834 mL
5 mM41.668 µL208.342 µL416.684 µL2.083 mL4.167 mL
10 mM20.834 µL104.171 µL208.342 µL1.042 mL2.083 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.27
Correction Factor (280 nm)0.34
Extinction coefficient (cm -1 M -1)100000
Excitation (nm)544
Emission (nm)570

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References


View all 45 references: Citation Explorer
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Authors: Andreeva N, Nyamaa A, Haupt H, Gross J, Mazurek B.
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Nontypeable Haemophilus influenzae strain 2019 produces a biofilm containing N-acetylneuraminic acid that may mimic sialylated O-linked glycans
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Journal: Int J Biochem Cell Biol (2001): 1151
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Journal: Microbiology (2001): 299