AAT Bioquest

# 5-TAMRA [5-Carboxytetramethylrhodamine] *CAS 91809-66-4*

5-TAMRA is the purified single isomer of 5(6)-TAMRA. It is widely used to label peptides and proteins. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-TAMRA and 6-TAMRA might affect some biological properties of the underlying conjugates. Currently AAT Bioquest is using a proprietary process to synthesize isomer-free 5-TAMRA without the conventional chromatographic purification.

## Calculators

### Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-TAMRA [5-Carboxytetramethylrhodamine] *CAS 91809-66-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

 0.1 mg 0.5 mg 1 mg 5 mg 10 mg 1 mM 232.315 µL 1.162 mL 2.323 mL 11.616 mL 23.232 mL 5 mM 46.463 µL 232.315 µL 464.63 µL 2.323 mL 4.646 mL 10 mM 23.232 µL 116.158 µL 232.315 µL 1.162 mL 2.323 mL

### Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

 Mass (Calculate) Molecular weight Volume (Calculate) Concentration (Calculate) Moles fgpgngµgmgg / g/mol = fLpLnLµLmLL x fMpMnMµMmMM = fmolpmolnmolµmolmmolmol

## Product family

 Name Excitation (nm) Emission (nm) Extinction coefficient (cm -1 M -1) Correction Factor (280 nm) 5-CR110 [5-Carboxyrhodamine 110] *Single isomer* 499 525 84000 0.091 5-CR6G [5-Carboxyrhodamine 6G] *Single isomer* 522 546 94000 0.214 6-TAMRA [6-Carboxytetramethylrhodamine] *CAS 91809-67-5* 552 578 90000 0.178 5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3* 578 604 82000 0.168 5-TRITC [Tetramethylrhodamine-5-isothiocyanate] *CAS 80724-19-2* 544 570 100000 0.34 5-FAM [5-Carboxyfluorescein] *CAS 76823-03-5* 493 517 83000 0.178 5-SFX [5-FAM-X, SE] *CAS 148356-00-7* 493 517 83000 0.178 5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7* 491 516 73000 0.35 5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5* 498 517 800001 0.35 Show More (3)

## Citations

View all 4 citations: Citation Explorer
Amikacin liposome inhalation suspension (ALIS) penetrates non-tuberculous mycobacterial biofilms and enhances amikacin uptake into macrophages
Authors: Zhang, Jimin and Leifer, Franziska and Rose, Sasha and Chun, Dung Yu and Thaisz, Jill and Herr, Tracey and Nashed, Mary and Joseph, Jayanthi and Perkins, Walter R and DiPetrillo, Keith
Journal: Frontiers in microbiology (2018)
Design and Characterization of Two Bifunctional Cryptophane A-Based Host Molecules for Xenon Magnetic Resonance Imaging Applications
Authors: Rossella, Federica and Rose, Honor May and Witte, Christopher and Jayapaul, Jabadurai and Schr&ouml;der, Leif
Journal: ChemPlusChem (2014): 1463--1471
Human erythrocytes as drug carriers: loading efficiency and side effects of hypotonic dialysis, chlorpromazine treatment and fusion with liposomes
Authors: Favretto, ME and Cluitmans, JCA and Bosman, GJCGM and Brock, R
Journal: Journal of Controlled Release (2013): 343--351
Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Preus, S&oslash;ren and Wilhelmsson, L Marcus
Journal: ChemBioChem (2012): 1990--2001

## References

View all 96 references: Citation Explorer
Metal-enhanced fluorescence-based RNA sensing
Authors: Aslan K, Huang J, Wilson GM, Geddes CD.
Journal: J Am Chem Soc (2006): 4206
G Quadruplex-Based FRET Probes with the Thrombin-Binding Aptamer (TBA) Sequence Designed for the Efficient Fluorometric Detection of the Potassium Ion
Authors: Nagatoishi S, Nojima T, Galezowska E, Juskowiak B, Takenaka S.
Journal: Chembiochem (2006): 1730
A targeted protease substrate for a quantitative determination of protease activities in the endolysosomal pathway
Authors: Fischer R, Bachle D, Fotin-Mleczek M, Jung G, Kalbacher H, Brock R.
Journal: Chembiochem (2006): 1428
Electrostatic-gated transport in chemically modified glass nanopore electrodes
Authors: Wang G, Zhang B, Wayment JR, Harris JM, White HS.
Journal: J Am Chem Soc (2006): 7679
Fluorescence anisotropy and FRET studies of G-quadruplex formation in presence of different cations
Authors: Juskowiak B, Galezowska E, Zawadzka A, Gluszynska A, Takenaka S.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 835
Page updated on September 19, 2024

## Ordering information

 Price Unit size 10 mg100 mg1 g Catalog Number 363364365 Quantity

 Telephone 1-800-990-8053 Fax 1-800-609-2943 Email sales@aatbio.com International See distributors Bulk request Inquire Custom size Inquire Technical Support Contact us Purchase order Send to sales@aatbio.com Shipping Standard overnight for United States, inquire for international

430.45

DMSO

0.32

0.178

90000

552

578

## Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

### Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

91809-66-4

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