Name: 5-CR6G [5-Carboxyrhodamine 6G] *Single isomer*
Brand: AAT Bioquest
SKU: 331
Price: 348 USD
Availability: InStock

5-CR6G

5-Carboxyrhodamine 6G; Single isomer

5-CR6G is the purified single isomer of 5(6)-CR6G. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-CR6G and 6-CR6G might significantly affect some biological properties of the underlying conjugates. This isomer is predominantly used to label small molecules, peptides and proteins.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
331	10 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-CR6G [5-Carboxyrhodamine 6G] *Single isomer* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	202.032 µL	1.01 mL	2.02 mL	10.102 mL	20.203 mL
5 mM	40.406 µL	202.032 µL	404.065 µL	2.02 mL	4.041 mL
10 mM	20.203 µL	101.016 µL	202.032 µL	1.01 mL	2.02 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	494.97 g/mol	=	mL	x	mM	=	mmol

References

View all 6 references: Citation Explorer

Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides

Authors:

Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.

Journal:

Proc Natl Acad Sci U S A (2005): 5926

Modifying the adsorption behavior of polyamidoamine dendrimers at silica surfaces investigated by total internal reflection fluorescence correlation spectroscopy

Authors:

McCain KS, Schluesche P, Harris JM.

Journal:

Anal Chem (2004): 930

High-power blue/UV light-emitting diodes as excitation sources for sensitive detection

Authors:

Kuo JS, Kuyper CL, Allen PB, Fiorini GS, Chiu DT.

Journal:

Electrophoresis (2004): 3796

Fluorescence resonance energy transfer (FRET) and competing processes in donor-acceptor substituted DNA strands: a comparative study of ensemble and single-molecule data

Authors:

Dietrich A, Buschmann V, Muller C, Sauer M.

Journal:

J Biotechnol (2002): 211

Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant

Authors:

Arezi B, Hansen CJ, Hogrefe HH.

Journal:

J Mol Biol (2002): 719

Physical properties

Molecular weight	494.97
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.24
Correction factor (280 nm)	0.214
Extinction coefficient (cm ^-1 M ^-1)	94000
Excitation (nm)	522
Emission (nm)	546

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	180144-69-8

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Page updated on October 13, 2025