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6-CR6G [6-Carboxyrhodamine 6G] *Single isomer*

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Physical properties
Molecular weight494.97
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.24
Correction Factor (280 nm)0.214
Extinction coefficient (cm -1 M -1)94000
Excitation (nm)522
Emission (nm)546
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Show More (40)

OverviewpdfSDSpdfProtocol


CAS
180144-68-7
Molecular weight
494.97
Correction Factor (260 nm)
0.24
Correction Factor (280 nm)
0.214
Extinction coefficient (cm -1 M -1)
94000
Excitation (nm)
522
Emission (nm)
546
6-CR6G is the other purified single isomer of 5(6)-CR6G. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-CR6G and 6-CR6G might significantly affect some biological properties of the underlying conjugates. Complimentary to 5-CR6G, 6-CR6G is predominantly used for labeling nucleotides and nucleic acids.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-CR6G [6-Carboxyrhodamine 6G] *Single isomer* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM202.032 µL1.01 mL2.02 mL10.102 mL20.203 mL
5 mM40.406 µL202.032 µL404.065 µL2.02 mL4.041 mL
10 mM20.203 µL101.016 µL202.032 µL1.01 mL2.02 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.24
Correction Factor (280 nm)0.214
Extinction coefficient (cm -1 M -1)94000
Excitation (nm)522
Emission (nm)546

Images


Citations


View all 1 citations: Citation Explorer
Rotational motion of rhodamine 6G tethered to actin through oligo (ethylene glycol) linkers studied by frequency-domain fluorescence anisotropy
Authors: Wazawa, Tetsuichi and Morimoto, Nobuyuki and Nagai, Takeharu and Suzuki, Makoto
Journal: Biophysics and Physicobiology (2015): 87--102

References


View all 6 references: Citation Explorer
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
High-power blue/UV light-emitting diodes as excitation sources for sensitive detection
Authors: Kuo JS, Kuyper CL, Allen PB, Fiorini GS, Chiu DT.
Journal: Electrophoresis (2004): 3796
Modifying the adsorption behavior of polyamidoamine dendrimers at silica surfaces investigated by total internal reflection fluorescence correlation spectroscopy
Authors: McCain KS, Schluesche P, Harris JM.
Journal: Anal Chem (2004): 930
Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant
Authors: Arezi B, Hansen CJ, Hogrefe HH.
Journal: J Mol Biol (2002): 719
Fluorescence resonance energy transfer (FRET) and competing processes in donor-acceptor substituted DNA strands: a comparative study of ensemble and single-molecule data
Authors: Dietrich A, Buschmann V, Muller C, Sauer M.
Journal: J Biotechnol (2002): 211
Optimization of spectroscopic and electrophoretic properties of energy transfer primers
Authors: Hung SC, Mathies RA, Glazer AN.
Journal: Anal Biochem (1997): 78