5-CR110 [5-Carboxyrhodamine 110] *Single isomer*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 410.81 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.24 |
Correction Factor (280 nm) | 0.091 |
Extinction coefficient (cm -1 M -1) | 84000 |
Excitation (nm) | 499 |
Emission (nm) | 525 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | ![]() ![]() |
See also: Rhodamines and Rhodamine Derivatives
Molecular weight 410.81 | Correction Factor (260 nm) 0.24 | Correction Factor (280 nm) 0.091 | Extinction coefficient (cm -1 M -1) 84000 | Excitation (nm) 499 | Emission (nm) 525 |
Compared to fluorescein labeling reagents such as FITC and FAM, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength. They are photostable alternative reagents superior to FITC and FAM. Carboxyrhodamine 110 is also referred as Rhodamine Green™ Carboxylic Acid.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 5-CR110 [5-Carboxyrhodamine 110] *Single isomer* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 243.422 µL | 1.217 mL | 2.434 mL | 12.171 mL | 24.342 mL |
5 mM | 48.684 µL | 243.422 µL | 486.843 µL | 2.434 mL | 4.868 mL |
10 mM | 24.342 µL | 121.711 µL | 243.422 µL | 1.217 mL | 2.434 mL |
Molarity calculator
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.24 |
Correction Factor (280 nm) | 0.091 |
Extinction coefficient (cm -1 M -1) | 84000 |
Excitation (nm) | 499 |
Emission (nm) | 525 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
6-CR110 [6-Carboxyrhodamine 110] *Single isomer* | 499 | 525 | 84000 | 0.091 |
5-CR6G [5-Carboxyrhodamine 6G] *Single isomer* | 522 | 546 | 94000 | 0.214 |
5-TAMRA [5-Carboxytetramethylrhodamine] *CAS 91809-66-4* | 552 | 578 | 90000 | 0.178 |
5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3* | 578 | 604 | 82000 | 0.168 |
5-TRITC [Tetramethylrhodamine-5-isothiocyanate] *CAS 80724-19-2* | 544 | 570 | 100000 | 0.34 |
5-FAM [5-Carboxyfluorescein] *CAS 76823-03-5* | 493 | 517 | 83000 | 0.178 |
5-SFX [5-FAM-X, SE] *CAS 148356-00-7* | 493 | 517 | 83000 | 0.178 |
5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7* | 491 | 516 | 73000 | 0.35 |
5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5* | 498 | 517 | 800001 | 0.35 |
Show More (3) |
Citations
View all 1 citations: Citation Explorer
Synthesis of rhodamines from fluoresceins using Pd-catalyzed C--N cross-coupling
Authors: Grimm, Jonathan B and Lavis, Luke D
Journal: Organic letters (2011): 6354--6357
Authors: Grimm, Jonathan B and Lavis, Luke D
Journal: Organic letters (2011): 6354--6357
References
View all 16 references: Citation Explorer
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Authors: Li X, Li Z, Marti AA, Jockusch S, Stevens N, Akins DL, Turro NJ, Ju J.
Journal: Photochem Photobiol Sci (2006): 896
Trace fluorescent labeling for high-throughput crystallography
Authors: Forsythe E, Achari A, Pusey ML.
Journal: Acta Crystallogr D Biol Crystallogr (2006): 339
Authors: Forsythe E, Achari A, Pusey ML.
Journal: Acta Crystallogr D Biol Crystallogr (2006): 339
Two-photon excitation induced fluorescence of a trifluorophore-labeled DNA
Authors: Jockusch S, Li Z, Ju J, Turro NJ.
Journal: Photochem Photobiol (2005): 238
Authors: Jockusch S, Li Z, Ju J, Turro NJ.
Journal: Photochem Photobiol (2005): 238
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
An integrative approach for the optical sequencing of single DNA molecules
Authors: Ramanathan A, Huff EJ, Lamers CC, Potamousis KD, Forrest DK, Schwartz DC.
Journal: Anal Biochem (2004): 227
Authors: Ramanathan A, Huff EJ, Lamers CC, Potamousis KD, Forrest DK, Schwartz DC.
Journal: Anal Biochem (2004): 227
High-power blue/UV light-emitting diodes as excitation sources for sensitive detection
Authors: Kuo JS, Kuyper CL, Allen PB, Fiorini GS, Chiu DT.
Journal: Electrophoresis (2004): 3796
Authors: Kuo JS, Kuyper CL, Allen PB, Fiorini GS, Chiu DT.
Journal: Electrophoresis (2004): 3796
Modifying the adsorption behavior of polyamidoamine dendrimers at silica surfaces investigated by total internal reflection fluorescence correlation spectroscopy
Authors: McCain KS, Schluesche P, Harris JM.
Journal: Anal Chem (2004): 930
Authors: McCain KS, Schluesche P, Harris JM.
Journal: Anal Chem (2004): 930
An economical and preparative orthogonal solid phase synthesis of fluorescein and rhodamine derivatized peptides: FRET substrates for the Staphylococcus aureus sortase SrtA transpeptidase reaction
Authors: Kruger RG, Dostal P, McCafferty DG.
Journal: Chem Commun (Camb) (2002): 2092
Authors: Kruger RG, Dostal P, McCafferty DG.
Journal: Chem Commun (Camb) (2002): 2092
Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant
Authors: Arezi B, Hansen CJ, Hogrefe HH.
Journal: J Mol Biol (2002): 719
Authors: Arezi B, Hansen CJ, Hogrefe HH.
Journal: J Mol Biol (2002): 719
Fluorescence resonance energy transfer (FRET) and competing processes in donor-acceptor substituted DNA strands: a comparative study of ensemble and single-molecule data
Authors: Dietrich A, Buschmann V, Muller C, Sauer M.
Journal: J Biotechnol (2002): 211
Authors: Dietrich A, Buschmann V, Muller C, Sauer M.
Journal: J Biotechnol (2002): 211
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