5-CR110 [5-Carboxyrhodamine 110] Single isomer

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Physical properties

Molecular weight	410.81
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.24
Correction Factor (280 nm)	0.091
Extinction coefficient (cm ^-1 M ^-1)	84000
Excitation (nm)	499
Emission (nm)	525

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

410.81

Correction Factor (260 nm)

0.24

Correction Factor (280 nm)

0.091

Extinction coefficient (cm ^-1 M ^-1)

84000

Excitation (nm)

499

Emission (nm)

525

Compared to fluorescein labeling reagents such as FITC and FAM, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength. They are photostable alternative reagents superior to FITC and FAM. Carboxyrhodamine 110 is also referred as Rhodamine Green™ Carboxylic Acid.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-CR110 [5-Carboxyrhodamine 110] *Single isomer* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	243.422 µL	1.217 mL	2.434 mL	12.171 mL	24.342 mL
5 mM	48.684 µL	243.422 µL	486.843 µL	2.434 mL	4.868 mL
10 mM	24.342 µL	121.711 µL	243.422 µL	1.217 mL	2.434 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.24
Correction Factor (280 nm)	0.091
Extinction coefficient (cm ^-1 M ^-1)	84000
Excitation (nm)	499
Emission (nm)	525

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
6-CR110 [6-Carboxyrhodamine 110] Single isomer	499	525	84000	0.091
5-CR6G [5-Carboxyrhodamine 6G] Single isomer	522	546	94000	0.214
5-TAMRA [5-Carboxytetramethylrhodamine] CAS 91809-66-4	552	578	90000	0.178
5-ROX [5-Carboxy-X-rhodamine] CAS 216699-35-3	578	604	82000	0.168
5-TRITC [Tetramethylrhodamine-5-isothiocyanate] CAS 80724-19-2	544	570	100000	0.34
5-FAM [5-Carboxyfluorescein] CAS 76823-03-5	493	517	83000	0.178
5-SFX [5-FAM-X, SE] CAS 148356-00-7	493	517	83000	0.178
5-FITC [Fluorescein-5-isothiocyanate] CAS 3326-32-7	491	516	73000	0.35
5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] CAS 51306-35-5	498	517	80000¹	0.35
5-IAF [5-Iodoacetamidofluorescein] CAS 63368-54-7	498	517	80000¹	0.35
DiBAC4(5) [Bis-(1,3-dibutylbarbituric acid)pentamethine oxonol]	591	615	-	-
DiSBAC2(5) [Bis-(1,3-diethylthiobarbituric acid)pentamethine oxonol]	636	655	-	-
Show More (3)

Images

Figure 1. Chemical structure for 5-CR110 [5-Carboxyrhodamine 110] *Single isomer*

Citations

View all 1 citations: Citation Explorer

Synthesis of rhodamines from fluoresceins using Pd-catalyzed C--N cross-coupling
Authors: Grimm, Jonathan B and Lavis, Luke D
Journal: Organic letters (2011): 6354--6357

References

View all 16 references: Citation Explorer

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Authors: Li X, Li Z, Marti AA, Jockusch S, Stevens N, Akins DL, Turro NJ, Ju J.
Journal: Photochem Photobiol Sci (2006): 896

Trace fluorescent labeling for high-throughput crystallography
Authors: Forsythe E, Achari A, Pusey ML.
Journal: Acta Crystallogr D Biol Crystallogr (2006): 339

Two-photon excitation induced fluorescence of a trifluorophore-labeled DNA
Authors: Jockusch S, Li Z, Ju J, Turro NJ.
Journal: Photochem Photobiol (2005): 238

Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926

An integrative approach for the optical sequencing of single DNA molecules
Authors: Ramanathan A, Huff EJ, Lamers CC, Potamousis KD, Forrest DK, Schwartz DC.
Journal: Anal Biochem (2004): 227

High-power blue/UV light-emitting diodes as excitation sources for sensitive detection
Authors: Kuo JS, Kuyper CL, Allen PB, Fiorini GS, Chiu DT.
Journal: Electrophoresis (2004): 3796

Modifying the adsorption behavior of polyamidoamine dendrimers at silica surfaces investigated by total internal reflection fluorescence correlation spectroscopy
Authors: McCain KS, Schluesche P, Harris JM.
Journal: Anal Chem (2004): 930

An economical and preparative orthogonal solid phase synthesis of fluorescein and rhodamine derivatized peptides: FRET substrates for the Staphylococcus aureus sortase SrtA transpeptidase reaction
Authors: Kruger RG, Dostal P, McCafferty DG.
Journal: Chem Commun (Camb) (2002): 2092

Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant
Authors: Arezi B, Hansen CJ, Hogrefe HH.
Journal: J Mol Biol (2002): 719

Fluorescence resonance energy transfer (FRET) and competing processes in donor-acceptor substituted DNA strands: a comparative study of ensemble and single-molecule data
Authors: Dietrich A, Buschmann V, Muller C, Sauer M.
Journal: J Biotechnol (2002): 211