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5-CR110 [5-Carboxyrhodamine 110] *Single isomer*

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Physical properties
Molecular weight410.81
Spectral properties
Correction Factor (260 nm)0.24
Correction Factor (280 nm)0.091
Extinction coefficient (cm -1 M -1)84000
Excitation (nm)499
Emission (nm)525
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Show More (40)


Molecular weight
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Compared to fluorescein labeling reagents such as FITC and FAM, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength. They are photostable alternative reagents superior to FITC and FAM. Carboxyrhodamine 110 is also referred as Rhodamine Green™ Carboxylic Acid.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-CR110 [5-Carboxyrhodamine 110] *Single isomer* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM243.422 µL1.217 mL2.434 mL12.171 mL24.342 mL
5 mM48.684 µL243.422 µL486.843 µL2.434 mL4.868 mL
10 mM24.342 µL121.711 µL243.422 µL1.217 mL2.434 mL

Molarity calculator

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Spectral properties

Correction Factor (260 nm)0.24
Correction Factor (280 nm)0.091
Extinction coefficient (cm -1 M -1)84000
Excitation (nm)499
Emission (nm)525



View all 1 citations: Citation Explorer
Synthesis of rhodamines from fluoresceins using Pd-catalyzed C--N cross-coupling
Authors: Grimm, Jonathan B and Lavis, Luke D
Journal: Organic letters (2011): 6354--6357


View all 16 references: Citation Explorer
Combinatorial fluorescence energy transfer molecular beacons for probing nucleic acid sequences
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Trace fluorescent labeling for high-throughput crystallography
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Journal: Acta Crystallogr D Biol Crystallogr (2006): 339
Two-photon excitation induced fluorescence of a trifluorophore-labeled DNA
Authors: Jockusch S, Li Z, Ju J, Turro NJ.
Journal: Photochem Photobiol (2005): 238
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
An integrative approach for the optical sequencing of single DNA molecules
Authors: Ramanathan A, Huff EJ, Lamers CC, Potamousis KD, Forrest DK, Schwartz DC.
Journal: Anal Biochem (2004): 227
High-power blue/UV light-emitting diodes as excitation sources for sensitive detection
Authors: Kuo JS, Kuyper CL, Allen PB, Fiorini GS, Chiu DT.
Journal: Electrophoresis (2004): 3796
Modifying the adsorption behavior of polyamidoamine dendrimers at silica surfaces investigated by total internal reflection fluorescence correlation spectroscopy
Authors: McCain KS, Schluesche P, Harris JM.
Journal: Anal Chem (2004): 930
An economical and preparative orthogonal solid phase synthesis of fluorescein and rhodamine derivatized peptides: FRET substrates for the Staphylococcus aureus sortase SrtA transpeptidase reaction
Authors: Kruger RG, Dostal P, McCafferty DG.
Journal: Chem Commun (Camb) (2002): 2092
Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant
Authors: Arezi B, Hansen CJ, Hogrefe HH.
Journal: J Mol Biol (2002): 719
Fluorescence resonance energy transfer (FRET) and competing processes in donor-acceptor substituted DNA strands: a comparative study of ensemble and single-molecule data
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Journal: J Biotechnol (2002): 211