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5-CR110 [5-Carboxyrhodamine 110] *Single isomer*
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Application notes
Compared to fluorescein labeling reagents such as FITC and FAM, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength. They are photostable alternative reagents superior to FITC and FAM. Carboxyrhodamine 110 is also referred as Rhodamine Green™ Carboxylic Acid.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 5-CR110 [5-Carboxyrhodamine 110] *Single isomer* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 243.422 µL | 1.217 mL | 2.434 mL | 12.171 mL | 24.342 mL |
| 5 mM | 48.684 µL | 243.422 µL | 486.843 µL | 2.434 mL | 4.868 mL |
| 10 mM | 24.342 µL | 121.711 µL | 243.422 µL | 1.217 mL | 2.434 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| 6-CR110 [6-Carboxyrhodamine 110] *Single isomer* | 499 | 525 | 84000 | 0.091 |
| 5-CR6G [5-Carboxyrhodamine 6G] *Single isomer* | 522 | 546 | 94000 | 0.214 |
| 5-TAMRA [5-Carboxytetramethylrhodamine] *CAS 91809-66-4* | 552 | 578 | 90000 | 0.178 |
| 5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3* | 578 | 604 | 82000 | 0.168 |
| 5-TRITC [Tetramethylrhodamine-5-isothiocyanate] *CAS 80724-19-2* | 544 | 570 | 100000 | 0.34 |
| 5-FAM [5-Carboxyfluorescein] *CAS 76823-03-5* | 493 | 517 | 83000 | 0.178 |
| 5-SFX [5-FAM-X, SE] *CAS 148356-00-7* | 493 | 517 | 83000 | 0.178 |
| 5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7* | 491 | 516 | 73000 | 0.35 |
| 5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5* | 498 | 517 | 800001 | 0.35 |
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Citations
View all 1 citations: Citation Explorer
Synthesis of rhodamines from fluoresceins using Pd-catalyzed C--N cross-coupling
Authors: Grimm, Jonathan B and Lavis, Luke D
Journal: Organic letters (2011): 6354--6357
Authors: Grimm, Jonathan B and Lavis, Luke D
Journal: Organic letters (2011): 6354--6357
References
View all 16 references: Citation Explorer
Combinatorial fluorescence energy transfer molecular beacons for probing nucleic acid sequences
Authors: Li X, Li Z, Marti AA, Jockusch S, Stevens N, Akins DL, Turro NJ, Ju J.
Journal: Photochem Photobiol Sci (2006): 896
Authors: Li X, Li Z, Marti AA, Jockusch S, Stevens N, Akins DL, Turro NJ, Ju J.
Journal: Photochem Photobiol Sci (2006): 896
Trace fluorescent labeling for high-throughput crystallography
Authors: Forsythe E, Achari A, Pusey ML.
Journal: Acta Crystallogr D Biol Crystallogr (2006): 339
Authors: Forsythe E, Achari A, Pusey ML.
Journal: Acta Crystallogr D Biol Crystallogr (2006): 339
Two-photon excitation induced fluorescence of a trifluorophore-labeled DNA
Authors: Jockusch S, Li Z, Ju J, Turro NJ.
Journal: Photochem Photobiol (2005): 238
Authors: Jockusch S, Li Z, Ju J, Turro NJ.
Journal: Photochem Photobiol (2005): 238
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
An integrative approach for the optical sequencing of single DNA molecules
Authors: Ramanathan A, Huff EJ, Lamers CC, Potamousis KD, Forrest DK, Schwartz DC.
Journal: Anal Biochem (2004): 227
Authors: Ramanathan A, Huff EJ, Lamers CC, Potamousis KD, Forrest DK, Schwartz DC.
Journal: Anal Biochem (2004): 227