Name: 5(6)-Carboxyrhodamine 6G ethylenediamine
Brand: AAT Bioquest
SKU: 344
Price: 348 USD
Availability: InStock

5(6)-Carboxyrhodamine 6G ethylenediamine

5(6)-Carboxyrhodamine 6G ethylenediamine is a building block for developing rhodamine bioconjugates. In general, rhodamine 6G conjugates have higher fluorescence quantum yields than tetramethylrhodamine conjugates, as well as good photostability. The excitation and emission wavelengths of rhodamine 6G fall between those of fluorescein and tetramethylrhodamine derivatives, making 5(6)-CR6G another color choice for the multicolor fluorescence imaging applications. 5(6)-CR6G is the mixture of two carboxy rhodamine 6G isomers.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
344	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-Carboxyrhodamine 6G ethylenediamine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	137.244 µL	686.219 µL	1.372 mL	6.862 mL	13.724 mL
5 mM	27.449 µL	137.244 µL	274.488 µL	1.372 mL	2.745 mL
10 mM	13.724 µL	68.622 µL	137.244 µL	686.219 µL	1.372 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	728.63 g/mol	=	mL	x	mM	=	mmol

References

View all 6 references: Citation Explorer

Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides

Authors:

Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.

Journal:

Proc Natl Acad Sci U S A (2005): 5926

Modifying the adsorption behavior of polyamidoamine dendrimers at silica surfaces investigated by total internal reflection fluorescence correlation spectroscopy

Authors:

McCain KS, Schluesche P, Harris JM.

Journal:

Anal Chem (2004): 930

High-power blue/UV light-emitting diodes as excitation sources for sensitive detection

Authors:

Kuo JS, Kuyper CL, Allen PB, Fiorini GS, Chiu DT.

Journal:

Electrophoresis (2004): 3796

Fluorescence resonance energy transfer (FRET) and competing processes in donor-acceptor substituted DNA strands: a comparative study of ensemble and single-molecule data

Authors:

Dietrich A, Buschmann V, Muller C, Sauer M.

Journal:

J Biotechnol (2002): 211

Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant

Authors:

Arezi B, Hansen CJ, Hogrefe HH.

Journal:

J Mol Biol (2002): 719

Physical properties

Molecular weight	728.63
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.24
Correction factor (280 nm)	0.214
Extinction coefficient (cm ^-1 M ^-1)	94000
Excitation (nm)	522
Emission (nm)	546

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on September 20, 2025