5(6)-Carboxyrhodamine 6G ethylenediamine
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 728.63 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.24 |
Correction Factor (280 nm) | 0.214 |
Extinction coefficient (cm -1 M -1) | 94000 |
Excitation (nm) | 522 |
Emission (nm) | 546 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | ![]() ![]() |
See also: Rhodamines and Rhodamine Derivatives
Molecular weight 728.63 | Correction Factor (260 nm) 0.24 | Correction Factor (280 nm) 0.214 | Extinction coefficient (cm -1 M -1) 94000 | Excitation (nm) 522 | Emission (nm) 546 |
5(6)-Carboxyrhodamine 6G ethylenediamine is a building block for developing rhodamine bioconjugates. In general, rhodamine 6G conjugates have higher fluorescence quantum yields than tetramethylrhodamine conjugates, as well as good photostability. The excitation and emission wavelengths of rhodamine 6G fall between those of fluorescein and tetramethylrhodamine derivatives, making 5(6)-CR6G another color choice for the multicolor fluorescence imaging applications. 5(6)-CR6G is the mixture of two carboxy rhodamine 6G isomers.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-Carboxyrhodamine 6G ethylenediamine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 137.244 µL | 686.219 µL | 1.372 mL | 6.862 mL | 13.724 mL |
5 mM | 27.449 µL | 137.244 µL | 274.488 µL | 1.372 mL | 2.745 mL |
10 mM | 13.724 µL | 68.622 µL | 137.244 µL | 686.219 µL | 1.372 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.24 |
Correction Factor (280 nm) | 0.214 |
Extinction coefficient (cm -1 M -1) | 94000 |
Excitation (nm) | 522 |
Emission (nm) | 546 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
5(6)-Carboxyrhodamine 6G cadaverine | 522 | 546 | 94000 | 0.24 | 0.214 |
Citations
View all 1 citations: Citation Explorer
Rotational motion of rhodamine 6G tethered to actin through oligo (ethylene glycol) linkers studied by frequency-domain fluorescence anisotropy
Authors: Wazawa, Tetsuichi and Morimoto, Nobuyuki and Nagai, Takeharu and Suzuki, Makoto
Journal: Biophysics and Physicobiology (2015): 87--102
Authors: Wazawa, Tetsuichi and Morimoto, Nobuyuki and Nagai, Takeharu and Suzuki, Makoto
Journal: Biophysics and Physicobiology (2015): 87--102
References
View all 6 references: Citation Explorer
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
High-power blue/UV light-emitting diodes as excitation sources for sensitive detection
Authors: Kuo JS, Kuyper CL, Allen PB, Fiorini GS, Chiu DT.
Journal: Electrophoresis (2004): 3796
Authors: Kuo JS, Kuyper CL, Allen PB, Fiorini GS, Chiu DT.
Journal: Electrophoresis (2004): 3796
Modifying the adsorption behavior of polyamidoamine dendrimers at silica surfaces investigated by total internal reflection fluorescence correlation spectroscopy
Authors: McCain KS, Schluesche P, Harris JM.
Journal: Anal Chem (2004): 930
Authors: McCain KS, Schluesche P, Harris JM.
Journal: Anal Chem (2004): 930
Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant
Authors: Arezi B, Hansen CJ, Hogrefe HH.
Journal: J Mol Biol (2002): 719
Authors: Arezi B, Hansen CJ, Hogrefe HH.
Journal: J Mol Biol (2002): 719
Fluorescence resonance energy transfer (FRET) and competing processes in donor-acceptor substituted DNA strands: a comparative study of ensemble and single-molecule data
Authors: Dietrich A, Buschmann V, Muller C, Sauer M.
Journal: J Biotechnol (2002): 211
Authors: Dietrich A, Buschmann V, Muller C, Sauer M.
Journal: J Biotechnol (2002): 211
Optimization of spectroscopic and electrophoretic properties of energy transfer primers
Authors: Hung SC, Mathies RA, Glazer AN.
Journal: Anal Biochem (1997): 78
Authors: Hung SC, Mathies RA, Glazer AN.
Journal: Anal Biochem (1997): 78
Application notes
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A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets