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5(6)-Carboxyrhodamine 6G ethylenediamine

5(6)-Carboxyrhodamine 6G ethylenediamine is a building block for developing rhodamine bioconjugates. In general, rhodamine 6G conjugates have higher fluorescence quantum yields than tetramethylrhodamine conjugates, as well as good photostability. The excitation and emission wavelengths of rhodamine 6G fall between those of fluorescein and tetramethylrhodamine derivatives, making 5(6)-CR6G another color choice for the multicolor fluorescence imaging applications. 5(6)-CR6G is the mixture of two carboxy rhodamine 6G isomers.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-Carboxyrhodamine 6G ethylenediamine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM137.244 µL686.219 µL1.372 mL6.862 mL13.724 mL
5 mM27.449 µL137.244 µL274.488 µL1.372 mL2.745 mL
10 mM13.724 µL68.622 µL137.244 µL686.219 µL1.372 mL

Molarity calculator

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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
5(6)-Carboxyrhodamine 6G cadaverine522546940000.240.214

Citations

View all 1 citations: Citation Explorer
Rotational motion of rhodamine 6G tethered to actin through oligo (ethylene glycol) linkers studied by frequency-domain fluorescence anisotropy
Authors: Wazawa, Tetsuichi and Morimoto, Nobuyuki and Nagai, Takeharu and Suzuki, Makoto
Journal: Biophysics and Physicobiology (2015): 87--102

References

View all 6 references: Citation Explorer
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
Modifying the adsorption behavior of polyamidoamine dendrimers at silica surfaces investigated by total internal reflection fluorescence correlation spectroscopy
Authors: McCain KS, Schluesche P, Harris JM.
Journal: Anal Chem (2004): 930
High-power blue/UV light-emitting diodes as excitation sources for sensitive detection
Authors: Kuo JS, Kuyper CL, Allen PB, Fiorini GS, Chiu DT.
Journal: Electrophoresis (2004): 3796
Fluorescence resonance energy transfer (FRET) and competing processes in donor-acceptor substituted DNA strands: a comparative study of ensemble and single-molecule data
Authors: Dietrich A, Buschmann V, Muller C, Sauer M.
Journal: J Biotechnol (2002): 211
Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant
Authors: Arezi B, Hansen CJ, Hogrefe HH.
Journal: J Mol Biol (2002): 719
Page updated on November 9, 2024

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Physical properties

Molecular weight

728.63

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.24

Correction Factor (280 nm)

0.214

Extinction coefficient (cm -1 M -1)

94000

Excitation (nm)

522

Emission (nm)

546

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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