5(6)-Carboxyrhodamine 6G ethylenediamine

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Physical properties

Molecular weight	728.63
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.24
Correction Factor (280 nm)	0.214
Extinction coefficient (cm ^-1 M ^-1)	94000
Excitation (nm)	522
Emission (nm)	546

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

728.63

Correction Factor (260 nm)

0.24

Correction Factor (280 nm)

0.214

Extinction coefficient (cm ^-1 M ^-1)

94000

Excitation (nm)

522

Emission (nm)

546

5(6)-Carboxyrhodamine 6G ethylenediamine is a building block for developing rhodamine bioconjugates. In general, rhodamine 6G conjugates have higher fluorescence quantum yields than tetramethylrhodamine conjugates, as well as good photostability. The excitation and emission wavelengths of rhodamine 6G fall between those of fluorescein and tetramethylrhodamine derivatives, making 5(6)-CR6G another color choice for the multicolor fluorescence imaging applications. 5(6)-CR6G is the mixture of two carboxy rhodamine 6G isomers.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-Carboxyrhodamine 6G ethylenediamine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	137.244 µL	686.219 µL	1.372 mL	6.862 mL	13.724 mL
5 mM	27.449 µL	137.244 µL	274.488 µL	1.372 mL	2.745 mL
10 mM	13.724 µL	68.622 µL	137.244 µL	686.219 µL	1.372 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.24
Correction Factor (280 nm)	0.214
Extinction coefficient (cm ^-1 M ^-1)	94000
Excitation (nm)	522
Emission (nm)	546

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (260 nm)	Correction Factor (280 nm)
5(6)-Carboxyrhodamine 6G cadaverine	522	546	94000	0.24	0.214

Images

Figure 1. Chemical structure for 5(6)-Carboxyrhodamine 6G ethylenediamine

Citations

View all 1 citations: Citation Explorer

Rotational motion of rhodamine 6G tethered to actin through oligo (ethylene glycol) linkers studied by frequency-domain fluorescence anisotropy
Authors: Wazawa, Tetsuichi and Morimoto, Nobuyuki and Nagai, Takeharu and Suzuki, Makoto
Journal: Biophysics and Physicobiology (2015): 87--102

References

View all 6 references: Citation Explorer

Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926

High-power blue/UV light-emitting diodes as excitation sources for sensitive detection
Authors: Kuo JS, Kuyper CL, Allen PB, Fiorini GS, Chiu DT.
Journal: Electrophoresis (2004): 3796

Modifying the adsorption behavior of polyamidoamine dendrimers at silica surfaces investigated by total internal reflection fluorescence correlation spectroscopy
Authors: McCain KS, Schluesche P, Harris JM.
Journal: Anal Chem (2004): 930

Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant
Authors: Arezi B, Hansen CJ, Hogrefe HH.
Journal: J Mol Biol (2002): 719

Fluorescence resonance energy transfer (FRET) and competing processes in donor-acceptor substituted DNA strands: a comparative study of ensemble and single-molecule data
Authors: Dietrich A, Buschmann V, Muller C, Sauer M.
Journal: J Biotechnol (2002): 211

Optimization of spectroscopic and electrophoretic properties of energy transfer primers
Authors: Hung SC, Mathies RA, Glazer AN.
Journal: Anal Biochem (1997): 78