5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3*
![Chemical structure for 5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3*](https://images.aatbio.com/products/figures-and-data/5-rox-5-carboxy-x-rhodamine-cas-216699-35-3/chemical-structure-of-5-rox-5-carboxy-x-rhodamine-cas-216699-35-3.svg)
| Overview |  SDSProtocol |
See also: Classic Dyes, Chemical Reagents, Dyes by Functional Group, Peptide and Oligonucleotide Labeling, Rhodamines and Rhodamine Derivatives
Molecular weight 534.60 | Correction Factor (280 nm) 0.168 | Extinction coefficient (cm -1 M -1) 82000 | Excitation (nm) 578 | Emission (nm) 604 |
5-ROX is the purified single isomer of 5(6)-ROX. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-ROX and 6-ROX might significantly affect some biological properties of the underlying conjugates. 5-ROX is predominantly used to label peptides and proteins. Currently AAT Bioquest is using a proprietary process to synthesize isomer-free 5-ROX without the conventional chromatographic purification. Cautions must be exercised to prevent the deterioration during storage.
Calculators
Common stock solution preparation
Table 1. Volume of appropriate solvent needed to reconstitute specific mass of 5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 187.056 µL | 935.279 µL | 1.871 mL | 9.353 mL | 18.706 mL |
| 5 mM | 37.411 µL | 187.056 µL | 374.111 µL | 1.871 mL | 3.741 mL |
| 10 mM | 18.706 µL | 93.528 µL | 187.056 µL | 935.279 µL | 1.871 mL |
Molarity calculator
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Spectrum
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Spectral properties
| Correction Factor (280 nm) | 0.168 |
| Extinction coefficient (cm -1 M -1) | 82000 |
| Excitation (nm) | 578 |
| Emission (nm) | 604 |
Images
Figure 1. Chemical structure for 5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3*
Citations
View all 1 citations: Citation Explorer
A novel cell penetrating peptide for the differentiation of human neural stem cells
Authors: Ma, Weili and Jin, Geun-woo and Gehret, Paul M and Chada, Neil C and Suh, Won Hyuk
Journal: Biomolecules (2018): 48
Authors: Ma, Weili and Jin, Geun-woo and Gehret, Paul M and Chada, Neil C and Suh, Won Hyuk
Journal: Biomolecules (2018): 48
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Journal: Proc Natl Acad Sci U S A (2004): 5488
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Journal: Bioconjug Chem (1999): 241
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