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5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3*

Chemical structure for 5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3*
Chemical structure for 5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3*
Ordering information
Price ()Discontinued
Catalog Number381
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight534.60
Spectral properties
Correction Factor (280 nm)0.168
Extinction coefficient (cm -1 M -1)82000
Excitation (nm)578
Emission (nm)604
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
534.60
Correction Factor (280 nm)
0.168
Extinction coefficient (cm -1 M -1)
82000
Excitation (nm)
578
Emission (nm)
604
5-ROX is the purified single isomer of 5(6)-ROX. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-ROX and 6-ROX might significantly affect some biological properties of the underlying conjugates. 5-ROX is predominantly used to label peptides and proteins. Currently AAT Bioquest is using a proprietary process to synthesize isomer-free 5-ROX without the conventional chromatographic purification. Cautions must be exercised to prevent the deterioration during storage.

Calculators


Common stock solution preparation

Table 1. Volume of appropriate solvent needed to reconstitute specific mass of 5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM187.056 µL935.279 µL1.871 mL9.353 mL18.706 mL
5 mM37.411 µL187.056 µL374.111 µL1.871 mL3.741 mL
10 mM18.706 µL93.528 µL187.056 µL935.279 µL1.871 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Correction Factor (280 nm)0.168
Extinction coefficient (cm -1 M -1)82000
Excitation (nm)578
Emission (nm)604

Citations


View all 1 citations: Citation Explorer
A novel cell penetrating peptide for the differentiation of human neural stem cells
Authors: Ma, Weili and Jin, Geun-woo and Gehret, Paul M and Chada, Neil C and Suh, Won Hyuk
Journal: Biomolecules (2018): 48

References


View all 11 references: Citation Explorer
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Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834
Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488
Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412
Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
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Journal: Invest Ophthalmol Vis Sci (2003): 1312
HLA-DRB fluorotyping by dark quenching and automated analysis
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Journal: Tissue Antigens (2001): 250
Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction--amplified short tandem repeats during denaturing capillary electrophoresis
Authors: Hahn M, Wilhelm J, Pingoud A.
Journal: Electrophoresis (2001): 2691
Design, synthesis, and spectroscopic properties of peptide-bridged fluorescence energy-transfer cassettes
Authors: Li Y, Glazer AN.
Journal: Bioconjug Chem (1999): 241
Differential display with carboxy-X-rhodamine-labeled primers and the selection of differentially amplified cDNA fragments without cloning
Authors: Yoshikawa Y, Mukai H, Asada K, Hino F, Kato I.
Journal: Anal Biochem (1998): 82
Comparison of fluorescence energy transfer primers with different donor-acceptor dye combinations
Authors: Hung SC, Mathies RA, Glazer AN.
Journal: Anal Biochem (1998): 32