5-ROX [5-Carboxy-X-rhodamine] CAS 216699-35-3

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Telephone	1-800-990-8053
Fax	1-800-609-2943
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Physical properties

Molecular weight	534.60
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.168
Extinction coefficient (cm ^-1 M ^-1)	82000
Excitation (nm)	578
Emission (nm)	604
Quantum yield	0.94¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

216699-35-3

Molecular weight

534.60

Correction Factor (280 nm)

0.168

Extinction coefficient (cm ^-1 M ^-1)

82000

Excitation (nm)

578

Emission (nm)

604

Quantum yield

0.94¹

5-ROX is the purified single isomer of 5(6)-ROX. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-ROX and 6-ROX might significantly affect some biological properties of the underlying conjugates. 5-ROX is predominantly used to label peptides and proteins. Currently AAT Bioquest is using a proprietary process to synthesize isomer-free 5-ROX without the conventional chromatographic purification. Cautions must be exercised to prevent the deterioration during storage.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	187.056 µL	935.279 µL	1.871 mL	9.353 mL	18.706 mL
5 mM	37.411 µL	187.056 µL	374.111 µL	1.871 mL	3.741 mL
10 mM	18.706 µL	93.528 µL	187.056 µL	935.279 µL	1.871 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (280 nm)	0.168
Extinction coefficient (cm ^-1 M ^-1)	82000
Excitation (nm)	578
Emission (nm)	604
Quantum yield	0.94¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
5-CR110 [5-Carboxyrhodamine 110] Single isomer	499	525	84000	0.091
5-CR6G [5-Carboxyrhodamine 6G] Single isomer	522	546	94000	0.214
5-TAMRA [5-Carboxytetramethylrhodamine] CAS 91809-66-4	552	578	90000	0.178
6-ROX [6-Carboxy-X-rhodamine] CAS 194785-18-7	578	604	82000	0.168
5-TRITC [Tetramethylrhodamine-5-isothiocyanate] CAS 80724-19-2	544	570	100000	0.34
5-FAM [5-Carboxyfluorescein] CAS 76823-03-5	493	517	83000	0.178
5-SFX [5-FAM-X, SE] CAS 148356-00-7	493	517	83000	0.178
5-FITC [Fluorescein-5-isothiocyanate] CAS 3326-32-7	491	516	73000	0.35
5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] CAS 51306-35-5	498	517	80000¹	0.35
5-IAF [5-Iodoacetamidofluorescein] CAS 63368-54-7	498	517	80000¹	0.35
DiBAC4(5) [Bis-(1,3-dibutylbarbituric acid)pentamethine oxonol]	591	615	-	-
DiSBAC2(5) [Bis-(1,3-diethylthiobarbituric acid)pentamethine oxonol]	636	655	-	-
Show More (3)

Images

Figure 1. Chemical structure for 5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3*

Citations

View all 1 citations: Citation Explorer

A novel cell penetrating peptide for the differentiation of human neural stem cells
Authors: Ma, Weili and Jin, Geun-woo and Gehret, Paul M and Chada, Neil C and Suh, Won Hyuk
Journal: Biomolecules (2018): 48

References

View all 11 references: Citation Explorer

Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926

Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence
Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834

Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488

Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412

Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
Authors: Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: Invest Ophthalmol Vis Sci (2003): 1312

HLA-DRB fluorotyping by dark quenching and automated analysis
Authors: Slateva K, Elsner HA, Albis-Camps M, Blasczyk R.
Journal: Tissue Antigens (2001): 250

Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction--amplified short tandem repeats during denaturing capillary electrophoresis
Authors: Hahn M, Wilhelm J, Pingoud A.
Journal: Electrophoresis (2001): 2691

Design, synthesis, and spectroscopic properties of peptide-bridged fluorescence energy-transfer cassettes
Authors: Li Y, Glazer AN.
Journal: Bioconjug Chem (1999): 241

Differential display with carboxy-X-rhodamine-labeled primers and the selection of differentially amplified cDNA fragments without cloning
Authors: Yoshikawa Y, Mukai H, Asada K, Hino F, Kato I.
Journal: Anal Biochem (1998): 82

Comparison of fluorescence energy transfer primers with different donor-acceptor dye combinations
Authors: Hung SC, Mathies RA, Glazer AN.
Journal: Anal Biochem (1998): 32