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6-CR110 [6-Carboxyrhodamine 110] *Single isomer*

Chemical structure for 6-CR110 [6-Carboxyrhodamine 110] *Single isomer*
Chemical structure for 6-CR110 [6-Carboxyrhodamine 110] *Single isomer*
Ordering information
Price ()
Catalog Number323
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
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ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight410.81
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.24
Correction Factor (280 nm)0.091
Extinction coefficient (cm -1 M -1)84000
Excitation (nm)499
Emission (nm)525
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Show More (50)

OverviewpdfSDSpdfProtocol


Molecular weight
410.81
Correction Factor (260 nm)
0.24
Correction Factor (280 nm)
0.091
Extinction coefficient (cm -1 M -1)
84000
Excitation (nm)
499
Emission (nm)
525
Compared to fluorescein labeling reagents such as FITC and FAM, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength. They are photostable alternative reagents superior to FITC and FAM. Carboxyrhodamine 110 is also referred as Rhodamine Green™ Carboxylic Acid.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-CR110 [6-Carboxyrhodamine 110] *Single isomer* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM243.422 µL1.217 mL2.434 mL12.171 mL24.342 mL
5 mM48.684 µL243.422 µL486.843 µL2.434 mL4.868 mL
10 mM24.342 µL121.711 µL243.422 µL1.217 mL2.434 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.24
Correction Factor (280 nm)0.091
Extinction coefficient (cm -1 M -1)84000
Excitation (nm)499
Emission (nm)525

Citations


View all 1 citations: Citation Explorer
Synthesis of rhodamines from fluoresceins using Pd-catalyzed C--N cross-coupling
Authors: Grimm, Jonathan B and Lavis, Luke D
Journal: Organic letters (2011): 6354--6357

References


View all 16 references: Citation Explorer
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Journal: Photochem Photobiol Sci (2006): 896
Trace fluorescent labeling for high-throughput crystallography
Authors: Forsythe E, Achari A, Pusey ML.
Journal: Acta Crystallogr D Biol Crystallogr (2006): 339
Two-photon excitation induced fluorescence of a trifluorophore-labeled DNA
Authors: Jockusch S, Li Z, Ju J, Turro NJ.
Journal: Photochem Photobiol (2005): 238
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
An integrative approach for the optical sequencing of single DNA molecules
Authors: Ramanathan A, Huff EJ, Lamers CC, Potamousis KD, Forrest DK, Schwartz DC.
Journal: Anal Biochem (2004): 227
High-power blue/UV light-emitting diodes as excitation sources for sensitive detection
Authors: Kuo JS, Kuyper CL, Allen PB, Fiorini GS, Chiu DT.
Journal: Electrophoresis (2004): 3796
Modifying the adsorption behavior of polyamidoamine dendrimers at silica surfaces investigated by total internal reflection fluorescence correlation spectroscopy
Authors: McCain KS, Schluesche P, Harris JM.
Journal: Anal Chem (2004): 930
An economical and preparative orthogonal solid phase synthesis of fluorescein and rhodamine derivatized peptides: FRET substrates for the Staphylococcus aureus sortase SrtA transpeptidase reaction
Authors: Kruger RG, Dostal P, McCafferty DG.
Journal: Chem Commun (Camb) (2002): 2092
Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant
Authors: Arezi B, Hansen CJ, Hogrefe HH.
Journal: J Mol Biol (2002): 719
Fluorescence resonance energy transfer (FRET) and competing processes in donor-acceptor substituted DNA strands: a comparative study of ensemble and single-molecule data
Authors: Dietrich A, Buschmann V, Muller C, Sauer M.
Journal: J Biotechnol (2002): 211