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6-CR110 [6-Carboxyrhodamine 110] *Single isomer*

Compared to fluorescein labeling reagents such as FITC and FAM, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength. They are photostable alternative reagents superior to FITC and FAM. Carboxyrhodamine 110 is also referred as Rhodamine Green™ Carboxylic Acid.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-CR110 [6-Carboxyrhodamine 110] *Single isomer* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM243.422 µL1.217 mL2.434 mL12.171 mL24.342 mL
5 mM48.684 µL243.422 µL486.843 µL2.434 mL4.868 mL
10 mM24.342 µL121.711 µL243.422 µL1.217 mL2.434 mL

Molarity calculator

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Spectrum

Citations

View all 1 citations: Citation Explorer
Synthesis of rhodamines from fluoresceins using Pd-catalyzed C--N cross-coupling
Authors: Grimm, Jonathan B and Lavis, Luke D
Journal: Organic letters (2011): 6354--6357

References

View all 16 references: Citation Explorer
Combinatorial fluorescence energy transfer molecular beacons for probing nucleic acid sequences
Authors: Li X, Li Z, Marti AA, Jockusch S, Stevens N, Akins DL, Turro NJ, Ju J.
Journal: Photochem Photobiol Sci (2006): 896
Trace fluorescent labeling for high-throughput crystallography
Authors: Forsythe E, Achari A, Pusey ML.
Journal: Acta Crystallogr D Biol Crystallogr (2006): 339
Two-photon excitation induced fluorescence of a trifluorophore-labeled DNA
Authors: Jockusch S, Li Z, Ju J, Turro NJ.
Journal: Photochem Photobiol (2005): 238
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
An integrative approach for the optical sequencing of single DNA molecules
Authors: Ramanathan A, Huff EJ, Lamers CC, Potamousis KD, Forrest DK, Schwartz DC.
Journal: Anal Biochem (2004): 227
Page updated on December 1, 2024

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Physical properties

Molecular weight

410.81

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.24

Correction Factor (280 nm)

0.091

Extinction coefficient (cm -1 M -1)

84000

Excitation (nm)

499

Emission (nm)

525

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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