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6-TRITC [Tetramethylrhodamine-6-isothiocyanate] *CAS 80724-20-5*
6-TRITC (also called R isomer) is the other isomer of the TRITC labeling reagent that is widely used in preparing bioconjugates of proteins and nucleic acids. Complimentary to 5-TRITC, the 6-isomer is predominantly used in labeling nucleotides and nucleic acids. Cautions must b exercised for the storage of TRITC conjugates.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 6-TRITC [Tetramethylrhodamine-6-isothiocyanate] *CAS 80724-20-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 208.342 µL | 1.042 mL | 2.083 mL | 10.417 mL | 20.834 mL |
| 5 mM | 41.668 µL | 208.342 µL | 416.684 µL | 2.083 mL | 4.167 mL |
| 10 mM | 20.834 µL | 104.171 µL | 208.342 µL | 1.042 mL | 2.083 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
| 5-TRITC [Tetramethylrhodamine-5-isothiocyanate] *CAS 80724-19-2* | 544 | 570 | 100000 | 0.27 | 0.34 |
| 6-CR110 [6-Carboxyrhodamine 110] *Single isomer* | 499 | 525 | 84000 | 0.24 | 0.091 |
| 6-CR6G [6-Carboxyrhodamine 6G] *Single isomer* | 522 | 546 | 94000 | 0.24 | 0.214 |
| 6-ROX [6-Carboxy-X-rhodamine] *CAS 194785-18-7* | 578 | 604 | 82000 | - | 0.168 |
| 6-TAMRA [6-Carboxytetramethylrhodamine] *CAS 91809-67-5* | 552 | 578 | 90000 | 0.32 | 0.178 |
| TRITC-DHPE | 544 | 570 | 100000 | 0.27 | 0.34 |
References
View all 45 references: Citation Explorer
Recombinant human erythropoietin prevents ischemia-induced apoptosis and necrosis in explant cultures of the rat organ of Corti
Authors: Andreeva N, Nyamaa A, Haupt H, Gross J, Mazurek B.
Journal: Neurosci Lett (2006): 86
Authors: Andreeva N, Nyamaa A, Haupt H, Gross J, Mazurek B.
Journal: Neurosci Lett (2006): 86
Mechanism of advanced glycation end products-induced hyperpermeability in endothelial cells.
Authors: Guo XH, Huang QB, Chen B, Wang SY, Hou FF, Fu N.
Journal: Sheng Li Xue Bao (2005): 205
Authors: Guo XH, Huang QB, Chen B, Wang SY, Hou FF, Fu N.
Journal: Sheng Li Xue Bao (2005): 205
Musculature of an illoricate predatory rotifer Asplanchnopus multiceps as revealed by phalloidin fluorescence and confocal microscopy
Authors: Kotikova EA, Raikova OI, Reuter M, Gustafsson MK.
Journal: Tissue Cell (2004): 189
Authors: Kotikova EA, Raikova OI, Reuter M, Gustafsson MK.
Journal: Tissue Cell (2004): 189
Resonance energy transfer nanobiosensors based on affinity binding between apo-enzyme and its substrate
Authors: Chinnayelka S, McShane MJ.
Journal: Biomacromolecules (2004): 1657
Authors: Chinnayelka S, McShane MJ.
Journal: Biomacromolecules (2004): 1657
Nontypeable Haemophilus influenzae strain 2019 produces a biofilm containing N-acetylneuraminic acid that may mimic sialylated O-linked glycans
Authors: Greiner LL, Watanabe H, Phillips NJ, Shao J, Morgan A, Zaleski A, Gibson BW, Apicella MA.
Journal: Infect Immun (2004): 4249
Authors: Greiner LL, Watanabe H, Phillips NJ, Shao J, Morgan A, Zaleski A, Gibson BW, Apicella MA.
Journal: Infect Immun (2004): 4249
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