6-TRITC [Tetramethylrhodamine-6-isothiocyanate] CAS 80724-20-5

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Physical properties

Molecular weight	479.98
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.27
Correction Factor (280 nm)	0.34
Extinction coefficient (cm ^-1 M ^-1)	100000
Excitation (nm)	544
Emission (nm)	570

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

80724-20-5

Molecular weight

479.98

Correction Factor (260 nm)

0.27

Correction Factor (280 nm)

0.34

Extinction coefficient (cm ^-1 M ^-1)

100000

Excitation (nm)

544

Emission (nm)

570

6-TRITC (also called R isomer) is the other isomer of the TRITC labeling reagent that is widely used in preparing bioconjugates of proteins and nucleic acids. Complimentary to 5-TRITC, the 6-isomer is predominantly used in labeling nucleotides and nucleic acids. Cautions must b exercised for the storage of TRITC conjugates.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-TRITC [Tetramethylrhodamine-6-isothiocyanate] *CAS 80724-20-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	208.342 µL	1.042 mL	2.083 mL	10.417 mL	20.834 mL
5 mM	41.668 µL	208.342 µL	416.684 µL	2.083 mL	4.167 mL
10 mM	20.834 µL	104.171 µL	208.342 µL	1.042 mL	2.083 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Spectral properties

Correction Factor (260 nm)	0.27
Correction Factor (280 nm)	0.34
Extinction coefficient (cm ^-1 M ^-1)	100000
Excitation (nm)	544
Emission (nm)	570

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (260 nm)	Correction Factor (280 nm)
6-CR110 [6-Carboxyrhodamine 110] Single isomer	499	525	84000	0.24	0.091
6-CR6G [6-Carboxyrhodamine 6G] Single isomer	522	546	94000	0.24	0.214
6-TAMRA [6-Carboxytetramethylrhodamine] CAS 91809-67-5	552	578	90000	0.32	0.178
6-ROX [6-Carboxy-X-rhodamine] CAS 194785-18-7	578	604	82000	-	0.168
5-TRITC [Tetramethylrhodamine-5-isothiocyanate] CAS 80724-19-2	544	570	100000	0.27	0.34
6-FAM [6-Carboxyfluorescein] CAS 3301-79-9	493	517	83000	0.32	0.178
6-DTAF [6-(4,6-Dichlorotriazinyl)aminofluorescein] CAS 118357-32-7	498	517	80000¹	0.32	0.35
TRITC-DHPE	544	570	100000	0.27	0.34

Images

Figure 1. Chemical structure for 6-TRITC [Tetramethylrhodamine-6-isothiocyanate] *CAS 80724-20-5*

References

View all 45 references: Citation Explorer

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Journal: Tissue Cell (2004): 189

Resonance energy transfer nanobiosensors based on affinity binding between apo-enzyme and its substrate
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Journal: Biomacromolecules (2004): 1657

Nontypeable Haemophilus influenzae strain 2019 produces a biofilm containing N-acetylneuraminic acid that may mimic sialylated O-linked glycans
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Journal: Infect Immun (2004): 4249

Fluorescent dyes modify properties of proteins used in microvascular research
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Journal: Microcirculation (2003): 221

Maturity of pericytes in cerebral neocapillaries induced by growth factors: fluorescence immuno-histochemical analysis using confocal laser microscopy
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Journal: Clin Hemorheol Microcirc (2003): 417

The critical role of lymph nodes in corneal alloimmunization and graft rejection
Authors: Yamagami S, Dana MR.
Journal: Invest Ophthalmol Vis Sci (2001): 1293

C-terminal actin-binding sites of smooth muscle caldesmon switch actin between conformational states
Authors: Borovikov YS, Avrova SV, Vikhoreva NN, Vikhorev PG, Ermakov VS, Copel and O, Marston SB.
Journal: Int J Biochem Cell Biol (2001): 1151

Assessment of lectin-binding analysis for in situ detection of glycoconjugates in biofilm systems
Authors: Neu T, Swerhone GD, Lawrence JR.
Journal: Microbiology (2001): 299