6-TRITC [Tetramethylrhodamine-6-isothiocyanate] *CAS 80724-20-5*
Ordering information
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Additional ordering information
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Fax | 1-800-609-2943 |
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Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 479.98 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.27 |
Correction Factor (280 nm) | 0.34 |
Extinction coefficient (cm -1 M -1) | 100000 |
Excitation (nm) | 544 |
Emission (nm) | 570 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
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See also: Amine Reactive Dyes and Probes for Conjugation, Bioconjugation, Chemical Reagents, Classic Dyes, Dyes by Functional Group, Rhodamines and Rhodamine Derivatives
CAS 80724-20-5 | Molecular weight 479.98 | Correction Factor (260 nm) 0.27 | Correction Factor (280 nm) 0.34 | Extinction coefficient (cm -1 M -1) 100000 | Excitation (nm) 544 | Emission (nm) 570 |
6-TRITC (also called R isomer) is the other isomer of the TRITC labeling reagent that is widely used in preparing bioconjugates of proteins and nucleic acids. Complimentary to 5-TRITC, the 6-isomer is predominantly used in labeling nucleotides and nucleic acids. Cautions must b exercised for the storage of TRITC conjugates.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 6-TRITC [Tetramethylrhodamine-6-isothiocyanate] *CAS 80724-20-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 208.342 µL | 1.042 mL | 2.083 mL | 10.417 mL | 20.834 mL |
5 mM | 41.668 µL | 208.342 µL | 416.684 µL | 2.083 mL | 4.167 mL |
10 mM | 20.834 µL | 104.171 µL | 208.342 µL | 1.042 mL | 2.083 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.27 |
Correction Factor (280 nm) | 0.34 |
Extinction coefficient (cm -1 M -1) | 100000 |
Excitation (nm) | 544 |
Emission (nm) | 570 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
6-CR110 [6-Carboxyrhodamine 110] *Single isomer* | 499 | 525 | 84000 | 0.24 | 0.091 |
6-CR6G [6-Carboxyrhodamine 6G] *Single isomer* | 522 | 546 | 94000 | 0.24 | 0.214 |
6-TAMRA [6-Carboxytetramethylrhodamine] *CAS 91809-67-5* | 552 | 578 | 90000 | 0.32 | 0.178 |
6-ROX [6-Carboxy-X-rhodamine] *CAS 194785-18-7* | 578 | 604 | 82000 | - | 0.168 |
5-TRITC [Tetramethylrhodamine-5-isothiocyanate] *CAS 80724-19-2* | 544 | 570 | 100000 | 0.27 | 0.34 |
6-FAM [6-Carboxyfluorescein] *CAS 3301-79-9* | 493 | 517 | 83000 | 0.32 | 0.178 |
6-DTAF [6-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 118357-32-7* | 498 | 517 | 800001 | 0.32 | 0.35 |
TRITC-DHPE | 544 | 570 | 100000 | 0.27 | 0.34 |
Images
References
View all 45 references: Citation Explorer
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Journal: Neurosci Lett (2006): 86
Authors: Andreeva N, Nyamaa A, Haupt H, Gross J, Mazurek B.
Journal: Neurosci Lett (2006): 86
Mechanism of advanced glycation end products-induced hyperpermeability in endothelial cells.
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Journal: Sheng Li Xue Bao (2005): 205
Authors: Guo XH, Huang QB, Chen B, Wang SY, Hou FF, Fu N.
Journal: Sheng Li Xue Bao (2005): 205
Musculature of an illoricate predatory rotifer Asplanchnopus multiceps as revealed by phalloidin fluorescence and confocal microscopy
Authors: Kotikova EA, Raikova OI, Reuter M, Gustafsson MK.
Journal: Tissue Cell (2004): 189
Authors: Kotikova EA, Raikova OI, Reuter M, Gustafsson MK.
Journal: Tissue Cell (2004): 189
Resonance energy transfer nanobiosensors based on affinity binding between apo-enzyme and its substrate
Authors: Chinnayelka S, McShane MJ.
Journal: Biomacromolecules (2004): 1657
Authors: Chinnayelka S, McShane MJ.
Journal: Biomacromolecules (2004): 1657
Nontypeable Haemophilus influenzae strain 2019 produces a biofilm containing N-acetylneuraminic acid that may mimic sialylated O-linked glycans
Authors: Greiner LL, Watanabe H, Phillips NJ, Shao J, Morgan A, Zaleski A, Gibson BW, Apicella MA.
Journal: Infect Immun (2004): 4249
Authors: Greiner LL, Watanabe H, Phillips NJ, Shao J, Morgan A, Zaleski A, Gibson BW, Apicella MA.
Journal: Infect Immun (2004): 4249
Fluorescent dyes modify properties of proteins used in microvascular research
Authors: Bingaman S, Huxley VH, Rumbaut RE.
Journal: Microcirculation (2003): 221
Authors: Bingaman S, Huxley VH, Rumbaut RE.
Journal: Microcirculation (2003): 221
Maturity of pericytes in cerebral neocapillaries induced by growth factors: fluorescence immuno-histochemical analysis using confocal laser microscopy
Authors: Jariyapongskul A, Nakano A, Yamaguchi S, Nageswari K, Niimi H.
Journal: Clin Hemorheol Microcirc (2003): 417
Authors: Jariyapongskul A, Nakano A, Yamaguchi S, Nageswari K, Niimi H.
Journal: Clin Hemorheol Microcirc (2003): 417
The critical role of lymph nodes in corneal alloimmunization and graft rejection
Authors: Yamagami S, Dana MR.
Journal: Invest Ophthalmol Vis Sci (2001): 1293
Authors: Yamagami S, Dana MR.
Journal: Invest Ophthalmol Vis Sci (2001): 1293
C-terminal actin-binding sites of smooth muscle caldesmon switch actin between conformational states
Authors: Borovikov YS, Avrova SV, Vikhoreva NN, Vikhorev PG, Ermakov VS, Copel and O, Marston SB.
Journal: Int J Biochem Cell Biol (2001): 1151
Authors: Borovikov YS, Avrova SV, Vikhoreva NN, Vikhorev PG, Ermakov VS, Copel and O, Marston SB.
Journal: Int J Biochem Cell Biol (2001): 1151
Assessment of lectin-binding analysis for in situ detection of glycoconjugates in biofilm systems
Authors: Neu T, Swerhone GD, Lawrence JR.
Journal: Microbiology (2001): 299
Authors: Neu T, Swerhone GD, Lawrence JR.
Journal: Microbiology (2001): 299
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)