TRITC-DHPE
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1236.69 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.27 |
Correction Factor (280 nm) | 0.34 |
Extinction coefficient (cm -1 M -1) | 100000 |
Excitation (nm) | 544 |
Emission (nm) | 570 |
Storage, safety and handling
H-phrase | H301, H311, H331 |
Hazard symbol | T |
Intended use | Research Use Only (RUO) |
R-phrase | R23, R24, R25 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | ![]() ![]() |
See also: Rhodamines and Rhodamine Derivatives
Molecular weight 1236.69 | Correction Factor (260 nm) 0.27 | Correction Factor (280 nm) 0.34 | Extinction coefficient (cm -1 M -1) 100000 | Excitation (nm) 544 | Emission (nm) 570 |
TRITC DHPE is a phospholipid that is labeled on the head group with the bright, red-fluorescent TRITC dye. Rhodamine-labeled phospholipids have been used as tracers for membrane traffic during endocytosis and for lipid processing in hepatocytes. TRITC DHPE can be used as an energy transfer acceptor from NBD, BODIPY and fluorescein lipid probes. The longer emission wavelength of the TRITC dye provides superior separation of the donor and acceptor emission signals in resonance energy transfer microscopy.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of TRITC-DHPE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 80.861 µL | 404.305 µL | 808.61 µL | 4.043 mL | 8.086 mL |
5 mM | 16.172 µL | 80.861 µL | 161.722 µL | 808.61 µL | 1.617 mL |
10 mM | 8.086 µL | 40.431 µL | 80.861 µL | 404.305 µL | 808.61 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.27 |
Correction Factor (280 nm) | 0.34 |
Extinction coefficient (cm -1 M -1) | 100000 |
Excitation (nm) | 544 |
Emission (nm) | 570 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
5-TRITC [Tetramethylrhodamine-5-isothiocyanate] *CAS 80724-19-2* | 544 | 570 | 100000 | 0.27 | 0.34 |
6-TRITC [Tetramethylrhodamine-6-isothiocyanate] *CAS 80724-20-5* | 544 | 570 | 100000 | 0.27 | 0.34 |
Fluorescein DHPE | 498 | 517 | 800001 | 0.32 | 0.35 |
OG488 DHPE | 498 | 526 | 76000 | 0.31 | 0.12 |
Citations
View all 3 citations: Citation Explorer
Highly Robust Multilamellar Lipid Vesicles Generated through Intervesicular Self-assembly Mediated by Hydrolyzed Collagen Peptides
Authors: Koo, Bon Il and Rahman, Rafia Tasnim and Jang, Jihui and Lee, Dong Jae and Lee, Jun Bae and Nam, Yoon Sung
Journal: (2023)
Authors: Koo, Bon Il and Rahman, Rafia Tasnim and Jang, Jihui and Lee, Dong Jae and Lee, Jun Bae and Nam, Yoon Sung
Journal: (2023)
The magnitude of LFA-1/ICAM-1 forces fine-tune TCR-triggered T cell activation
Authors: Ma, Victor Pui-Yan and Hu, Yuesong and Kellner, Anna V and Brockman, Joshua M and Velusamy, Arventh and Blanchard, Aaron T and Evavold, Brian D and Alon, Ronen and Salaita, Khalid
Journal: Science advances (2022): eabg4485
Authors: Ma, Victor Pui-Yan and Hu, Yuesong and Kellner, Anna V and Brockman, Joshua M and Velusamy, Arventh and Blanchard, Aaron T and Evavold, Brian D and Alon, Ronen and Salaita, Khalid
Journal: Science advances (2022): eabg4485
Development of PEGylated aspartic acid-modified liposome as a bone-targeting carrier for the delivery of paclitaxel and treatment of bone metastasis
Authors: Yamashita, Shugo and Katsumi, Hidemasa and Hibino, Nozomi and Isobe, Yugo and Yagi, Yumiko and Tanaka, Yuka and Yamada, Saki and Naito, Chihiro and Yamamoto, Akira
Journal: Biomaterials (2017)
Authors: Yamashita, Shugo and Katsumi, Hidemasa and Hibino, Nozomi and Isobe, Yugo and Yagi, Yumiko and Tanaka, Yuka and Yamada, Saki and Naito, Chihiro and Yamamoto, Akira
Journal: Biomaterials (2017)
References
View all 27 references: Citation Explorer
Permeabilization assay for antimicrobial peptides based on pore-spanning lipid membranes on nanoporous alumina
Authors: Neubacher H, Mey I, Carnarius C, Lazzara TD, Steinem C.
Journal: Langmuir (2014): 4767
Authors: Neubacher H, Mey I, Carnarius C, Lazzara TD, Steinem C.
Journal: Langmuir (2014): 4767
Membrane order parameters for interdigitated lipid bilayers measured via polarized total-internal-reflection fluorescence microscopy
Authors: Ngo AT, Jakubek ZJ, Lu Z, Joos B, Morris CE, Johnston LJ.
Journal: Biochim Biophys Acta (2014): 2861
Authors: Ngo AT, Jakubek ZJ, Lu Z, Joos B, Morris CE, Johnston LJ.
Journal: Biochim Biophys Acta (2014): 2861
Mesoporous gold and palladium nanoleaves from liquid-liquid interface: enhanced catalytic activity of the palladium analogue toward hydrazine-assisted room-temperature 4-nitrophenol reduction
Authors: Dutta S, Sarkar S, Ray C, Roy A, Sahoo R, Pal T.
Journal: ACS Appl Mater Interfaces (2014): 9134
Authors: Dutta S, Sarkar S, Ray C, Roy A, Sahoo R, Pal T.
Journal: ACS Appl Mater Interfaces (2014): 9134
Phase segregation of polymerizable lipids to construct filters for separating lipid-membrane-embedded species
Authors: Hu SK, Chen YM, Chao L.
Journal: Biomicrofluidics (2014): 52005
Authors: Hu SK, Chen YM, Chao L.
Journal: Biomicrofluidics (2014): 52005
Lipid reassembly in asymmetric Langmuir-Blodgett/Langmuir-Schaeffer bilayers
Authors: Yuan J, Hao C, Chen M, Berini P, Zou S.
Journal: Langmuir (2013): 221
Authors: Yuan J, Hao C, Chen M, Berini P, Zou S.
Journal: Langmuir (2013): 221
Coupling supported lipid bilayer electrophoresis with matrix-assisted laser desorption/ionization-mass spectrometry imaging
Authors: Pace HP, Sherrod SD, Monson CF, Russell DH, Cremer PS.
Journal: Anal Chem (2013): 6047
Authors: Pace HP, Sherrod SD, Monson CF, Russell DH, Cremer PS.
Journal: Anal Chem (2013): 6047
Calcein release behavior from liposomal bilayer; influence of physicochemical/mechanical/structural properties of lipids
Authors: Maherani B, Arab-Tehrany E, Kheirolomoom A, Geny D, Linder M.
Journal: Biochimie (2013): 2018
Authors: Maherani B, Arab-Tehrany E, Kheirolomoom A, Geny D, Linder M.
Journal: Biochimie (2013): 2018
In vivo digestion of bovine milk fat globules: effect of processing and interfacial structural changes. I. Gastric digestion
Authors: Gallier S, Cui J, Olson TD, Rutherfurd SM, Ye A, Moughan PJ, Singh H.
Journal: Food Chem (2013): 3273
Authors: Gallier S, Cui J, Olson TD, Rutherfurd SM, Ye A, Moughan PJ, Singh H.
Journal: Food Chem (2013): 3273
Rapid detection of several foodborne pathogens by F0F1-ATPase molecular motor biosensor
Authors: Zhang J, Li Z, Zhang H, Wang J, Liu Y, Chen G.
Journal: J Microbiol Methods (2013): 37
Authors: Zhang J, Li Z, Zhang H, Wang J, Liu Y, Chen G.
Journal: J Microbiol Methods (2013): 37
Formation and diffusivity characterization of supported lipid bilayers with complex lipid compositions
Authors: Simonsson L, Hook F.
Journal: Langmuir (2012): 10528
Authors: Simonsson L, Hook F.
Journal: Langmuir (2012): 10528
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