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Physical properties
Molecular weight1236.69
Spectral properties
Correction Factor (260 nm)0.27
Correction Factor (280 nm)0.34
Extinction coefficient (cm -1 M -1)100000
Excitation (nm)544
Emission (nm)570
Storage, safety and handling
H-phraseH301, H311, H331
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR23, R24, R25
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
TRITC DHPE is a phospholipid that is labeled on the head group with the bright, red-fluorescent TRITC dye. Rhodamine-labeled phospholipids have been used as tracers for membrane traffic during endocytosis and for lipid processing in hepatocytes. TRITC DHPE can be used as an energy transfer acceptor from NBD, BODIPY and fluorescein lipid probes. The longer emission wavelength of the TRITC dye provides superior separation of the donor and acceptor emission signals in resonance energy transfer microscopy.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of TRITC-DHPE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM80.861 µL404.305 µL808.61 µL4.043 mL8.086 mL
5 mM16.172 µL80.861 µL161.722 µL808.61 µL1.617 mL
10 mM8.086 µL40.431 µL80.861 µL404.305 µL808.61 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles


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Spectral properties

Correction Factor (260 nm)0.27
Correction Factor (280 nm)0.34
Extinction coefficient (cm -1 M -1)100000
Excitation (nm)544
Emission (nm)570

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
5-TRITC [Tetramethylrhodamine-5-isothiocyanate] *CAS 80724-19-2*5445701000000.270.34
6-TRITC [Tetramethylrhodamine-6-isothiocyanate] *CAS 80724-20-5*5445701000000.270.34
Fluorescein DHPE4985178000010.320.35
OG488 DHPE498526760000.310.12



View all 4 citations: Citation Explorer
Highly Robust Multilamellar Lipid Vesicles Generated through Intervesicular Self-assembly Mediated by Hydrolyzed Collagen Peptides
Authors: Koo, Bon Il and Rahman, Rafia Tasnim and Jang, Jihui and Lee, Dong Jae and Lee, Jun Bae and Nam, Yoon Sung
Journal: (2023)
The magnitude of LFA-1/ICAM-1 forces fine-tune TCR-triggered T cell activation
Authors: Ma, Victor Pui-Yan and Hu, Yuesong and Kellner, Anna V and Brockman, Joshua M and Velusamy, Arventh and Blanchard, Aaron T and Evavold, Brian D and Alon, Ronen and Salaita, Khalid
Journal: Science advances (2022): eabg4485
Development of PEGylated aspartic acid-modified liposome as a bone-targeting carrier for the delivery of paclitaxel and treatment of bone metastasis
Authors: Yamashita, Shugo and Katsumi, Hidemasa and Hibino, Nozomi and Isobe, Yugo and Yagi, Yumiko and Tanaka, Yuka and Yamada, Saki and Naito, Chihiro and Yamamoto, Akira
Journal: Biomaterials (2017)
Biomimetic Multilayered Lipid Nanovesicles for Potent Protein Vaccination
Authors: Koo, Bon Il and Lee, Dong Jae and Rahman, Rafia Tasnim and Nam, Yoon Sung
Journal: Advanced Healthcare Materials : 2304109


View all 27 references: Citation Explorer
Permeabilization assay for antimicrobial peptides based on pore-spanning lipid membranes on nanoporous alumina
Authors: Neubacher H, Mey I, Carnarius C, Lazzara TD, Steinem C.
Journal: Langmuir (2014): 4767
Membrane order parameters for interdigitated lipid bilayers measured via polarized total-internal-reflection fluorescence microscopy
Authors: Ngo AT, Jakubek ZJ, Lu Z, Joos B, Morris CE, Johnston LJ.
Journal: Biochim Biophys Acta (2014): 2861
Mesoporous gold and palladium nanoleaves from liquid-liquid interface: enhanced catalytic activity of the palladium analogue toward hydrazine-assisted room-temperature 4-nitrophenol reduction
Authors: Dutta S, Sarkar S, Ray C, Roy A, Sahoo R, Pal T.
Journal: ACS Appl Mater Interfaces (2014): 9134
Phase segregation of polymerizable lipids to construct filters for separating lipid-membrane-embedded species
Authors: Hu SK, Chen YM, Chao L.
Journal: Biomicrofluidics (2014): 52005
Lipid reassembly in asymmetric Langmuir-Blodgett/Langmuir-Schaeffer bilayers
Authors: Yuan J, Hao C, Chen M, Berini P, Zou S.
Journal: Langmuir (2013): 221
Coupling supported lipid bilayer electrophoresis with matrix-assisted laser desorption/ionization-mass spectrometry imaging
Authors: Pace HP, Sherrod SD, Monson CF, Russell DH, Cremer PS.
Journal: Anal Chem (2013): 6047
Calcein release behavior from liposomal bilayer; influence of physicochemical/mechanical/structural properties of lipids
Authors: Maherani B, Arab-Tehrany E, Kheirolomoom A, Geny D, Linder M.
Journal: Biochimie (2013): 2018
In vivo digestion of bovine milk fat globules: effect of processing and interfacial structural changes. I. Gastric digestion
Authors: Gallier S, Cui J, Olson TD, Rutherfurd SM, Ye A, Moughan PJ, Singh H.
Journal: Food Chem (2013): 3273
Rapid detection of several foodborne pathogens by F0F1-ATPase molecular motor biosensor
Authors: Zhang J, Li Z, Zhang H, Wang J, Liu Y, Chen G.
Journal: J Microbiol Methods (2013): 37
Formation and diffusivity characterization of supported lipid bilayers with complex lipid compositions
Authors: Simonsson L, Hook F.
Journal: Langmuir (2012): 10528