TRITC-DHPE

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Physical properties

Molecular weight	1236.69
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.27
Correction Factor (280 nm)	0.34
Extinction coefficient (cm ^-1 M ^-1)	100000
Excitation (nm)	544
Emission (nm)	570

Storage, safety and handling

H-phrase	H301, H311, H331
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R23, R24, R25
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Molecular weight

1236.69

Correction Factor (260 nm)

0.27

Correction Factor (280 nm)

0.34

Extinction coefficient (cm ^-1 M ^-1)

100000

Excitation (nm)

544

Emission (nm)

570

TRITC DHPE is a phospholipid that is labeled on the head group with the bright, red-fluorescent TRITC dye. Rhodamine-labeled phospholipids have been used as tracers for membrane traffic during endocytosis and for lipid processing in hepatocytes. TRITC DHPE can be used as an energy transfer acceptor from NBD, BODIPY and fluorescein lipid probes. The longer emission wavelength of the TRITC dye provides superior separation of the donor and acceptor emission signals in resonance energy transfer microscopy.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of TRITC-DHPE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	80.861 µL	404.305 µL	808.61 µL	4.043 mL	8.086 mL
5 mM	16.172 µL	80.861 µL	161.722 µL	808.61 µL	1.617 mL
10 mM	8.086 µL	40.431 µL	80.861 µL	404.305 µL	808.61 µL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.27
Correction Factor (280 nm)	0.34
Extinction coefficient (cm ^-1 M ^-1)	100000
Excitation (nm)	544
Emission (nm)	570

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (260 nm)	Correction Factor (280 nm)
5-TRITC [Tetramethylrhodamine-5-isothiocyanate] CAS 80724-19-2	544	570	100000	0.27	0.34
6-TRITC [Tetramethylrhodamine-6-isothiocyanate] CAS 80724-20-5	544	570	100000	0.27	0.34
Fluorescein DHPE	498	517	80000¹	0.32	0.35
OG488 DHPE	498	526	76000	0.31	0.12

Images

Figure 1. Chemical structure for TRITC-DHPE

Citations

View all 3 citations: Citation Explorer

Highly Robust Multilamellar Lipid Vesicles Generated through Intervesicular Self-assembly Mediated by Hydrolyzed Collagen Peptides
Authors: Koo, Bon Il and Rahman, Rafia Tasnim and Jang, Jihui and Lee, Dong Jae and Lee, Jun Bae and Nam, Yoon Sung
Journal: (2023)

The magnitude of LFA-1/ICAM-1 forces fine-tune TCR-triggered T cell activation
Authors: Ma, Victor Pui-Yan and Hu, Yuesong and Kellner, Anna V and Brockman, Joshua M and Velusamy, Arventh and Blanchard, Aaron T and Evavold, Brian D and Alon, Ronen and Salaita, Khalid
Journal: Science advances (2022): eabg4485

Development of PEGylated aspartic acid-modified liposome as a bone-targeting carrier for the delivery of paclitaxel and treatment of bone metastasis
Authors: Yamashita, Shugo and Katsumi, Hidemasa and Hibino, Nozomi and Isobe, Yugo and Yagi, Yumiko and Tanaka, Yuka and Yamada, Saki and Naito, Chihiro and Yamamoto, Akira
Journal: Biomaterials (2017)

References

View all 27 references: Citation Explorer

Permeabilization assay for antimicrobial peptides based on pore-spanning lipid membranes on nanoporous alumina
Authors: Neubacher H, Mey I, Carnarius C, Lazzara TD, Steinem C.
Journal: Langmuir (2014): 4767

Membrane order parameters for interdigitated lipid bilayers measured via polarized total-internal-reflection fluorescence microscopy
Authors: Ngo AT, Jakubek ZJ, Lu Z, Joos B, Morris CE, Johnston LJ.
Journal: Biochim Biophys Acta (2014): 2861

Mesoporous gold and palladium nanoleaves from liquid-liquid interface: enhanced catalytic activity of the palladium analogue toward hydrazine-assisted room-temperature 4-nitrophenol reduction
Authors: Dutta S, Sarkar S, Ray C, Roy A, Sahoo R, Pal T.
Journal: ACS Appl Mater Interfaces (2014): 9134

Phase segregation of polymerizable lipids to construct filters for separating lipid-membrane-embedded species
Authors: Hu SK, Chen YM, Chao L.
Journal: Biomicrofluidics (2014): 52005

Lipid reassembly in asymmetric Langmuir-Blodgett/Langmuir-Schaeffer bilayers
Authors: Yuan J, Hao C, Chen M, Berini P, Zou S.
Journal: Langmuir (2013): 221

Coupling supported lipid bilayer electrophoresis with matrix-assisted laser desorption/ionization-mass spectrometry imaging
Authors: Pace HP, Sherrod SD, Monson CF, Russell DH, Cremer PS.
Journal: Anal Chem (2013): 6047

Calcein release behavior from liposomal bilayer; influence of physicochemical/mechanical/structural properties of lipids
Authors: Maherani B, Arab-Tehrany E, Kheirolomoom A, Geny D, Linder M.
Journal: Biochimie (2013): 2018

In vivo digestion of bovine milk fat globules: effect of processing and interfacial structural changes. I. Gastric digestion
Authors: Gallier S, Cui J, Olson TD, Rutherfurd SM, Ye A, Moughan PJ, Singh H.
Journal: Food Chem (2013): 3273

Rapid detection of several foodborne pathogens by F0F1-ATPase molecular motor biosensor
Authors: Zhang J, Li Z, Zhang H, Wang J, Liu Y, Chen G.
Journal: J Microbiol Methods (2013): 37

Formation and diffusivity characterization of supported lipid bilayers with complex lipid compositions
Authors: Simonsson L, Hook F.
Journal: Langmuir (2012): 10528