Name: 5-IAF [5-Iodoacetamidofluorescein] *CAS 63368-54-7*
Brand: AAT Bioquest
SKU: 222
Price: 232 USD
Availability: InStock

5-IAF

5-Iodoacetamidofluorescein; CAS 63368-54-7

5-IAF is one of the most popular green fluorescent dyes for thiol modifications of proteins along with fluorescein-5-maleimide. The resulted bioconjugates are quite stable.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
222	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-IAF [5-Iodoacetamidofluorescein] *CAS 63368-54-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	194.081 µL	970.403 µL	1.941 mL	9.704 mL	19.408 mL
5 mM	38.816 µL	194.081 µL	388.161 µL	1.941 mL	3.882 mL
10 mM	19.408 µL	97.04 µL	194.081 µL	970.403 µL	1.941 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	515.25 g/mol	=	mL	x	mM	=	mmol

References

View all 10 references: Citation Explorer

Quantitation of reduced disulfide groups in monoclonal antibodies using 5-iodoacetamidofluorescein: a novel size exclusion-HPLC technique

Authors:

Muddukrishna SN, Chen A, Qi P, Smolenski MA.

Journal:

Appl Radiat Isot (1995): 1015

Na,K-ATPase labelled with 5-iodoacetamidofluorescein: E2-E1 conformational transition induced by different nucleotides

Authors:

Lopina OD, Pindel EV, Boldyrev AA.

Journal:

FEBS Lett (1990): 75

Studies on conformational changes in Na,K-ATPase labeled with 5-iodoacetamidofluorescein

Authors:

Steinberg M, Karlish SJ.

Journal:

J Biol Chem (1989): 2726

Identification of the 5-iodoacetamidofluorescein reporter site on the Na,K-ATPase

Authors:

Tyson PA, Steinberg M, Wallick ET, Kirley TL.

Journal:

J Biol Chem (1989): 726

Distances between 5-iodoacetamidofluorescein and the ATP and ouabain sites of (Na,K)-ATPase determined by fluorescence energy transfer

Authors:

Fortes PA, Aguilar R.

Journal:

Prog Clin Biol Res (1988): 197

Physical properties

Molecular weight	515.25
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	63368-54-7

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Page updated on October 12, 2025