5-ROX glycine *Fluorescence reference standard for PCR reactions*

5-ROX glycine

Fluorescence reference standard for PCR reactions

5-ROX glycine is used as a reference dye for performing PCR detections. Compared to the standard ROX dyes 5-ROX glycine has improved stability. The 5-ROX glycine has the similar spectral properties to those of 6-ROX.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
396	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of 5-ROX glycine *Fluorescence reference standard for PCR reactions* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	169.019 µL	845.094 µL	1.69 mL	8.451 mL	16.902 mL
5 mM	33.804 µL	169.019 µL	338.038 µL	1.69 mL	3.38 mL
10 mM	16.902 µL	84.509 µL	169.019 µL	845.094 µL	1.69 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	591.65 g/mol	=	mL	x	mM	=	mmol

References

View all 11 references: Citation Explorer

Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides

Authors:

Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.

Journal:

Proc Natl Acad Sci U S A (2005): 5926

Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis

Authors:

Faulds K, Smith WE, Graham D.

Journal:

Anal Chem (2004): 412

Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry

Authors:

Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.

Journal:

Proc Natl Acad Sci U S A (2004): 5488

Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence

Authors:

Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.

Journal:

Langmuir (2004): 6834

Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport

Authors:

Yang JH, Basinger SF, Gross RL, Wu SM.

Journal:

Invest Ophthalmol Vis Sci (2003): 1312

Physical properties

Molecular weight	591.65
Solvent	Water

Spectral properties

Correction factor (280 nm)	0.168
Extinction coefficient (cm ^-1 M ^-1)	82000
Excitation (nm)	578
Emission (nm)	604
Quantum yield	0.94 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -60 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on July 26, 2023