5-ROX, SE [5-Carboxy-X-rhodamine, succinimidyl ester] *CAS 209734-74-7*

5-ROX, SE

5-Carboxy-X-rhodamine, succinimidyl ester; CAS 209734-74-7

Single isomers of ROX are increasingly preferred for labeling peptides and nucleotides because they give better resolution in HPLC purifications that are often required in bioconjugations. Variable ratios of the 5- and 6-isomers often cause complications in the interpretation of labeling results and assay performances. 5-ROX is predominantly used to label peptides and proteins. Compared to other rhodamines, ROX is very unstable. Cautions must be exercised to prevent the deterioration during storage.

Protocol

COA

Catalog	Size	Price	Quantity
389	5 mg	Price
391	100 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-ROX, SE [5-Carboxy-X-rhodamine, succinimidyl ester] *CAS 209734-74-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	158.311 µL	791.553 µL	1.583 mL	7.916 mL	15.831 mL
5 mM	31.662 µL	158.311 µL	316.621 µL	1.583 mL	3.166 mL
10 mM	15.831 µL	79.155 µL	158.311 µL	791.553 µL	1.583 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	631.67 g/mol	=	mL	x	mM	=	mmol

References

View all 11 references: Citation Explorer

Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides

Authors:

Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.

Journal:

Proc Natl Acad Sci U S A (2005): 5926

Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis

Authors:

Faulds K, Smith WE, Graham D.

Journal:

Anal Chem (2004): 412

Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry

Authors:

Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.

Journal:

Proc Natl Acad Sci U S A (2004): 5488

Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence

Authors:

Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.

Journal:

Langmuir (2004): 6834

Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport

Authors:

Yang JH, Basinger SF, Gross RL, Wu SM.

Journal:

Invest Ophthalmol Vis Sci (2003): 1312

Physical properties

Molecular weight	631.67
Solvent	DMSO

Spectral properties

Correction factor (280 nm)	0.168
Extinction coefficient (cm ^-1 M ^-1)	82000
Excitation (nm)	578
Emission (nm)	604
Quantum yield	0.94 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	209734-74-7

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Page updated on October 13, 2025