Name: 5-TAMRA BCN
Brand: AAT Bioquest
SKU: 70512
Price: 115 USD
Availability: InStock

5-TAMRA BCN

5-TAMRA BCN is a bright, photostable orange-red fluorescent dye used for site-specific labeling of peptides and other biomolecules via copper-free click chemistry.

Bioorthogonal: Selective for azides or tetrazines with minimal biological interference.
Fast kinetics: BCN reacts more rapidly than most other click chemistries.
Catalyst-free: The reaction does not require a toxic metal catalyst such as copper.
High biocompatibility: The reaction occurs under physiological pH and room temperature.
Versatility: It can be used for site-specific modification of a wide range of macromolecules.

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
70512	1 mg	Price

Overview

5-TAMRA BCN is a clickable derivative of 5-Carboxytetramethylrhodamine (5-TAMRA), commonly used for labeling biomolecules such as peptides. 5-TAMRA is a well-characterized orange-red fluorescent dye known for its high brightness, pH-insensitive fluorescence, and good photostability. To minimize steric hindrance and reduce potential interference with biomolecular interactions after conjugation, the molecule incorporates a polyethylene glycol (PEG) spacer. The bicyclononyne (BCN) moiety enables strain-promoted azide–alkyne cycloaddition (SPAAC) with azido groups, forming stable triazole linkages under catalyst-free conditions. In addition, unlike dibenzocyclooctyne (DBCO), BCN also reacts efficiently with tetrazines through an inverse electron-demand Diels–Alder (IEDDA) reaction. This reaction is rapid, selective, and bioorthogonal, allowing labeling of biomolecules under physiological conditions without the need for metal catalysts or disruption of native biological processes.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-TAMRA BCN to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	121.218 µL	606.09 µL	1.212 mL	6.061 mL	12.122 mL
5 mM	24.244 µL	121.218 µL	242.436 µL	1.212 mL	2.424 mL
10 mM	12.122 µL	60.609 µL	121.218 µL	606.09 µL	1.212 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	824.96 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Unsaturated Phosphine Oxides for Modular Antibody Rebridging and Single Reagent Peptide-Cyclization-Bioconjugation.

Authors:

Stieger, Christian E and McMillan, Alastair J and de Geus, Mark A R and Arafiles, Jan Vincent V and Franz, Luise and Hackenberger, Christian P R

Journal:

Angewandte Chemie (International ed. in English) (2025): e202508656

Cyclooctyne-Functionalized N-Heterocyclic Carbenes for Click Coupling of Sensing Oligonucleotides to Gold Surfaces.

Authors:

Nalaoh, Phattananawee and Clark, Vincent and Arroyo-Currás, Netzahualcóyotl and Jenkins, David M

Journal:

ACS sensors (2025): 6039-6047

Effect of Click Chemistry Linkages on the Biological Behavior of Albumin-Binding 177Lu-DOTAGA-pIBA-LLP2A Analogues Targeting Melanoma.

Authors:

Li, Longbo and Wirrick, Aidan and Pun, Michael D and Lovingier, Christine L and Gallazzi, Fabio and Fong, Cyril O Y and Watkinson, Lisa and Carmack, Terry L and Rodgers, Mikayla and Tucker, Katherine and Ho, Khanh-Van and Anderson, Carolyn J

Journal:

Bioconjugate chemistry (2025): 2002-2012

Cyclooctyne End-Functionalized Poly(morpholine-2,5-dione)s.

Authors:

Schreiber, Johanna and Göppert, Natalie E and Stafast, Leanne M and Weber, Christine and Schubert, Ulrich S

Journal:

Macromolecular rapid communications (2025): e2400705

Bioorthogonal SERS-bioluminescence dual-modal imaging for real-time tracking of triple-negative breast cancer metastasis.

Authors:

Zhang, Wei and Wang, Sisi and Xing, Yanlong and Luo, Xianzhu and Wang, Rui and Yu, Fabiao

Journal:

Acta biomaterialia (2025): 431-443

Physical properties

Molecular weight	824.96
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	552
Emission (nm)	578

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on May 8, 2026