5-TAMRA C6 maleimide

5-TAMRA C6 maleimide is an excellent replacement for tetramethylrhodamine-5-maleimide. It selectively reacts with thiol groups of peptides, proteins and other biological molecules (such as thiol-modified oligonucleotides and small thiol-containing organic molecules). It might give higher conjugation yield than tetramethylrhodamine-5-maleimide under similar conjugation conditions. 5-TMR C6 maleimide has the spectral propertied identical to those of tetramethylrhodamine-5-maleimide, thus all the instrument settings of the tetramethylrhodamine-5-maleimide would be applicable to the applications of 5-TMR C6 maleimide conjugates.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
424	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-TAMRA C6 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	160.341 µL	801.706 µL	1.603 mL	8.017 mL	16.034 mL
5 mM	32.068 µL	160.341 µL	320.682 µL	1.603 mL	3.207 mL
10 mM	16.034 µL	80.171 µL	160.341 µL	801.706 µL	1.603 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	623.67 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

Yeast Surface-Displayed Quenchbody as a Novel Whole-Cell Biosensor for One-Step Detection of Influenza A (H1N1) Virus

Authors:

Seo, Yoonjoo and Zhou, Aijia and Nguyen, Thanh H and Wei, Na

Journal:

ACS Synthetic Biology (2024)

Isolation of a human SARS-CoV-2 neutralizing antibody from a synthetic phage library and its conversion to fluorescent biosensors

Authors:

Li, Haimei and Zhu, Bo and Li, Baowei and Chen, Limei and Ning, Xuerao and Dong, Hang and Liang, Jingru and Yang, Xueying and Dong, Jinhua and Ueda, Hiroshi

Journal:

Scientific reports (2022): 1--14

Interchromophoric Interactions Between TMR, Alexa, and BODIPY Fluorophores

Authors:

Donaphon, Bryan M

References

View all 21 references: Citation Explorer

Severing of F-actin by yeast cofilin is pH-independent

Authors:

Pavlov D, Muhlrad A, Cooper J, Wear M, Reisler E.

Journal:

Cell Motil Cytoskeleton (2006): 533

Fluorometric measurements of intermolecular distances between the alpha and beta subunits of the Na+/K+-ATPase

Authors:

Dempski RE, Hartung K, Friedrich T, Bamberg E.

Journal:

J Biol Chem. (2006)

4-aminopyridine prevents the conformational changes associated with P/C-type inactivation in Shaker channels

Authors:

Claydon TW, Vaid M, Rezazadeh S, Kehl SJ, Fedida D.

Journal:

J Pharmacol Exp Ther. (2006)

Both rotor and stator subunits are necessary for efficient binding of F1 to F0 in functionally assembled Escherichia coli ATP synthase

Authors:

Krebstakies T, Zimmermann B, Graber P, Altendorf K, Borsch M, Greie JC.

Journal:

J Biol Chem (2005): 33338

The beta subunit of the Na+/K+-ATPase follows the conformational state of the holoenzyme

Authors:

Dempski RE, Friedrich T, Bamberg E.

Journal:

J Gen Physiol (2005): 505

Physical properties

Molecular weight	623.67
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	552
Emission (nm)	578

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 13, 2025