5-TAMRA C6 maleimide
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 623.67 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.178 |
Extinction coefficient (cm -1 M -1) | 90000 |
Excitation (nm) | 552 |
Emission (nm) | 578 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
See also: Digital PCR, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), PCR Detection of Viral DNA/RNA
Molecular weight 623.67 | Correction Factor (260 nm) 0.32 | Correction Factor (280 nm) 0.178 | Extinction coefficient (cm -1 M -1) 90000 | Excitation (nm) 552 | Emission (nm) 578 |
5-TAMRA C6 maleimide is an excellent replacement for tetramethylrhodamine-5-maleimide. It selectively reacts with thiol groups of peptides, proteins and other biological molecules (such as thiol-modified oligonucleotides and small thiol-containing organic molecules). It might give higher conjugation yield than tetramethylrhodamine-5-maleimide under similar conjugation conditions. 5-TMR C6 maleimide has the spectral propertied identical to those of tetramethylrhodamine-5-maleimide, thus all the instrument settings of the tetramethylrhodamine-5-maleimide would be applicable to the applications of 5-TMR C6 maleimide conjugates.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 5-TAMRA C6 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 160.341 µL | 801.706 µL | 1.603 mL | 8.017 mL | 16.034 mL |
5 mM | 32.068 µL | 160.341 µL | 320.682 µL | 1.603 mL | 3.207 mL |
10 mM | 16.034 µL | 80.171 µL | 160.341 µL | 801.706 µL | 1.603 mL |
Molarity calculator
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.178 |
Extinction coefficient (cm -1 M -1) | 90000 |
Excitation (nm) | 552 |
Emission (nm) | 578 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
5(6)-TAMRA Maleimide [Tetramethylrhodamine-5-(and-6)-maleimide] *Mixed isomers* | 552 | 578 | 90000 | 0.32 | 0.178 |
6-TAMRA C6 maleimide | 552 | 578 | 90000 | 0.32 | 0.178 |
Citations
View all 2 citations: Citation Explorer
Isolation of a human SARS-CoV-2 neutralizing antibody from a synthetic phage library and its conversion to fluorescent biosensors
Authors: Li, Haimei and Zhu, Bo and Li, Baowei and Chen, Limei and Ning, Xuerao and Dong, Hang and Liang, Jingru and Yang, Xueying and Dong, Jinhua and Ueda, Hiroshi
Journal: Scientific reports (2022): 1--14
Authors: Li, Haimei and Zhu, Bo and Li, Baowei and Chen, Limei and Ning, Xuerao and Dong, Hang and Liang, Jingru and Yang, Xueying and Dong, Jinhua and Ueda, Hiroshi
Journal: Scientific reports (2022): 1--14
Interchromophoric Interactions Between TMR, Alexa, and BODIPY Fluorophores
Authors: Donaphon, Bryan M
Journal: (2018)
Authors: Donaphon, Bryan M
Journal: (2018)
References
View all 21 references: Citation Explorer
Severing of F-actin by yeast cofilin is pH-independent
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Journal: Cell Motil Cytoskeleton (2006): 533
Authors: Pavlov D, Muhlrad A, Cooper J, Wear M, Reisler E.
Journal: Cell Motil Cytoskeleton (2006): 533
Fluorometric measurements of intermolecular distances between the alpha and beta subunits of the Na+/K+-ATPase
Authors: Dempski RE, Hartung K, Friedrich T, Bamberg E.
Journal: J Biol Chem. (2006)
Authors: Dempski RE, Hartung K, Friedrich T, Bamberg E.
Journal: J Biol Chem. (2006)
4-aminopyridine prevents the conformational changes associated with P/C-type inactivation in Shaker channels
Authors: Claydon TW, Vaid M, Rezazadeh S, Kehl SJ, Fedida D.
Journal: J Pharmacol Exp Ther. (2006)
Authors: Claydon TW, Vaid M, Rezazadeh S, Kehl SJ, Fedida D.
Journal: J Pharmacol Exp Ther. (2006)
Both rotor and stator subunits are necessary for efficient binding of F1 to F0 in functionally assembled Escherichia coli ATP synthase
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Journal: J Biol Chem (2005): 33338
Authors: Krebstakies T, Zimmermann B, Graber P, Altendorf K, Borsch M, Greie JC.
Journal: J Biol Chem (2005): 33338
The beta subunit of the Na+/K+-ATPase follows the conformational state of the holoenzyme
Authors: Dempski RE, Friedrich T, Bamberg E.
Journal: J Gen Physiol (2005): 505
Authors: Dempski RE, Friedrich T, Bamberg E.
Journal: J Gen Physiol (2005): 505
Perturbation analysis of the voltage-sensitive conformational changes of the Na+/glucose cotransporter
Authors: Loo DD, Hirayama BA, Cha A, Bezanilla F, Wright EM.
Journal: J Gen Physiol (2005): 13
Authors: Loo DD, Hirayama BA, Cha A, Bezanilla F, Wright EM.
Journal: J Gen Physiol (2005): 13
Cytochrome c conformations resolved by the photon counting histogram: watching the alkaline transition with single-molecule sensitivity
Authors: Perroud TD, Bokoch MP, Zare RN.
Journal: Proc Natl Acad Sci U S A (2005): 17570
Authors: Perroud TD, Bokoch MP, Zare RN.
Journal: Proc Natl Acad Sci U S A (2005): 17570
Membrane topology of loop 13-14 of the Na+/glucose cotransporter (SGLT1): a SCAM and fluorescent labelling study
Authors: Gagnon DG, Holt A, Bourgeois F, Wallendorff B, Coady MJ, Lapointe JY.
Journal: Biochim Biophys Acta (2005): 173
Authors: Gagnon DG, Holt A, Bourgeois F, Wallendorff B, Coady MJ, Lapointe JY.
Journal: Biochim Biophys Acta (2005): 173
Distances between tropomyosin sites across the muscle thin filament using luminescence resonance energy transfer: evidence for tropomyosin flexibility
Authors: Chen Y, Lehrer SS.
Journal: Biochemistry (2004): 11491
Authors: Chen Y, Lehrer SS.
Journal: Biochemistry (2004): 11491
Crystal structure of monomeric actin in the ATP state. Structural basis of nucleotide-dependent actin dynamics
Authors: Graceffa P, Dominguez R.
Journal: J Biol Chem (2003): 34172
Authors: Graceffa P, Dominguez R.
Journal: J Biol Chem (2003): 34172
Application notes
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