5-TAMRA ethylenediamine

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Physical properties

Molecular weight	700.58
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	552
Emission (nm)	578

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

700.58

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.178

Extinction coefficient (cm ^-1 M ^-1)

90000

Excitation (nm)

552

Emission (nm)

578

5-TAMRA ethylenediamine is a building block for developing TAMRA bioconjugates. TAMRA is one of the most popular bright orange fluorophores used in various bioconjugations.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-TAMRA ethylenediamine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	142.739 µL	713.694 µL	1.427 mL	7.137 mL	14.274 mL
5 mM	28.548 µL	142.739 µL	285.478 µL	1.427 mL	2.855 mL
10 mM	14.274 µL	71.369 µL	142.739 µL	713.694 µL	1.427 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	552
Emission (nm)	578

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (260 nm)	Correction Factor (280 nm)
5-FAM ethylenediamine	493	517	83000	0.32	0.178
5-FITC ethylenediamine [Fluorescein thiocarbamylethylenediamine] CAS 75453-82-6	491	516	73000	-	0.35
5-TAMRA cadaverine	552	578	90000	0.32	0.178
6-TAMRA ethylenediamine	552	578	90000	0.32	0.178
5(6)-TAMRA [5(6)-Carboxytetramethylrhodamine] CAS 98181-63-6	552	578	90000	0.32	0.178
5-TAMRA, SE [5-Carboxytetramethylrhodamine, succinimidyl ester] CAS#: 150810-68-7	552	578	90000	0.32	0.178
5-TAMRA Maleimide [Tetramethylrhodamine-5-maleimide] CAS 154480-30-5	552	578	90000	0.32	0.178
5-TAMRA azide	552	578	90000	0.32	0.178
5-TAMRA alkyne	552	578	90000	0.32	0.178

Images

Figure 1. Chemical structure for 5-TAMRA ethylenediamine

Citations

View all 3 citations: Citation Explorer

Design and Characterization of Two Bifunctional Cryptophane A-Based Host Molecules for Xenon Magnetic Resonance Imaging Applications
Authors: Rossella, Federica and Rose, Honor May and Witte, Christopher and Jayapaul, Jabadurai and Schröder, Leif
Journal: ChemPlusChem (2014): 1463--1471

Human erythrocytes as drug carriers: loading efficiency and side effects of hypotonic dialysis, chlorpromazine treatment and fusion with liposomes
Authors: Favretto, ME and Cluitmans, JCA and Bosman, GJCGM and Brock, R
Journal: Journal of Controlled Release (2013): 343--351

Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Preus, Søren and Wilhelmsson, L Marcus
Journal: ChemBioChem (2012): 1990--2001

References

View all 96 references: Citation Explorer

Optical detection of DNA hybridization based on fluorescence quenching of tagged oligonucleotide probes by gold nanoparticles
Authors: Wu ZS, Jiang JH, Fu L, Shen GL, Yu RQ.
Journal: Anal Biochem (2006): 22

Masking oligonucleotides improve sensitivity of mutation detection based on guanine quenching
Authors: Maruyama T, Shinohara T, Hosogi T, Ichinose H, Kamiya N, Goto M.
Journal: Anal Biochem (2006): 8

A fluorescence polarization assay for screening inhibitors against the ribonuclease H activity of HIV-1 reverse transcriptase
Authors: Nakayama GR, Bingham P, Tan D, Maegley KA.
Journal: Anal Biochem (2006): 260

High efficiency micellar electrokinetic chromatography of hydrophobic analytes on poly(dimethylsiloxane) microchips
Authors: Roman GT, McDaniel K, Culbertson CT.
Journal: Analyst (2006): 194

Metal-enhanced fluorescence-based RNA sensing
Authors: Aslan K, Huang J, Wilson GM, Geddes CD.
Journal: J Am Chem Soc (2006): 4206

Fluorescence anisotropy and FRET studies of G-quadruplex formation in presence of different cations
Authors: Juskowiak B, Galezowska E, Zawadzka A, Gluszynska A, Takenaka S.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 835

Electrostatic-gated transport in chemically modified glass nanopore electrodes
Authors: Wang G, Zhang B, Wayment JR, Harris JM, White HS.
Journal: J Am Chem Soc (2006): 7679

A targeted protease substrate for a quantitative determination of protease activities in the endolysosomal pathway
Authors: Fischer R, Bachle D, Fotin-Mleczek M, Jung G, Kalbacher H, Brock R.
Journal: Chembiochem (2006): 1428

G Quadruplex-Based FRET Probes with the Thrombin-Binding Aptamer (TBA) Sequence Designed for the Efficient Fluorometric Detection of the Potassium Ion
Authors: Nagatoishi S, Nojima T, Galezowska E, Juskowiak B, Takenaka S.
Journal: Chembiochem (2006): 1730

Fluorescence properties of fluorescein, tetramethylrhodamine and Texas Red linked to a DNA aptamer
Authors: Unruh JR, Gokulrangan G, Wilson GS, Johnson CK.
Journal: Photochem Photobiol (2005): 682