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5-TAMRA Maleimide [Tetramethylrhodamine-5-maleimide] *CAS 154480-30-5*
Tetramethylrhodamine-5-maleimide is the purified single isomer of tetramethylrhodamine-5 (and 6)-maleimide mixed isomers. It is widely used for thiol modifications of peptides and proteins. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between the 5-isomer and 6-isomer might significantly affect some biological properties of the underlying conjugates. The 5-isomer is predominantly used to label peptides and proteins.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 5-TAMRA Maleimide [Tetramethylrhodamine-5-maleimide] *CAS 154480-30-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 207.68 µL | 1.038 mL | 2.077 mL | 10.384 mL | 20.768 mL |
| 5 mM | 41.536 µL | 207.68 µL | 415.36 µL | 2.077 mL | 4.154 mL |
| 10 mM | 20.768 µL | 103.84 µL | 207.68 µL | 1.038 mL | 2.077 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
| 5(6)-TAMRA [5(6)-Carboxytetramethylrhodamine] *CAS 98181-63-6* | 552 | 578 | 90000 | 0.32 | 0.178 |
| 5-TAMRA, SE [5-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-68-7* | 552 | 578 | 90000 | 0.32 | 0.178 |
| 6-TAMRA Maleimide [Tetramethylrhodamine-6-maleimide] *CAS 174568-68-4* | 552 | 578 | 90000 | 0.32 | 0.178 |
| 5-TAMRA alkyne | 552 | 578 | 90000 | 0.32 | 0.178 |
| 5-TAMRA azide | 552 | 578 | 90000 | 0.32 | 0.178 |
| 5-TAMRA ethylenediamine | 552 | 578 | 90000 | 0.32 | 0.178 |
References
View all 21 references: Citation Explorer
Severing of F-actin by yeast cofilin is pH-independent
Authors: Pavlov D, Muhlrad A, Cooper J, Wear M, Reisler E.
Journal: Cell Motil Cytoskeleton (2006): 533
Authors: Pavlov D, Muhlrad A, Cooper J, Wear M, Reisler E.
Journal: Cell Motil Cytoskeleton (2006): 533
Fluorometric measurements of intermolecular distances between the alpha and beta subunits of the Na+/K+-ATPase
Authors: Dempski RE, Hartung K, Friedrich T, Bamberg E.
Journal: J Biol Chem. (2006)
Authors: Dempski RE, Hartung K, Friedrich T, Bamberg E.
Journal: J Biol Chem. (2006)
4-aminopyridine prevents the conformational changes associated with P/C-type inactivation in Shaker channels
Authors: Claydon TW, Vaid M, Rezazadeh S, Kehl SJ, Fedida D.
Journal: J Pharmacol Exp Ther. (2006)
Authors: Claydon TW, Vaid M, Rezazadeh S, Kehl SJ, Fedida D.
Journal: J Pharmacol Exp Ther. (2006)
Both rotor and stator subunits are necessary for efficient binding of F1 to F0 in functionally assembled Escherichia coli ATP synthase
Authors: Krebstakies T, Zimmermann B, Graber P, Altendorf K, Borsch M, Greie JC.
Journal: J Biol Chem (2005): 33338
Authors: Krebstakies T, Zimmermann B, Graber P, Altendorf K, Borsch M, Greie JC.
Journal: J Biol Chem (2005): 33338
The beta subunit of the Na+/K+-ATPase follows the conformational state of the holoenzyme
Authors: Dempski RE, Friedrich T, Bamberg E.
Journal: J Gen Physiol (2005): 505
Authors: Dempski RE, Friedrich T, Bamberg E.
Journal: J Gen Physiol (2005): 505
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