5-TAMRA Maleimide [Tetramethylrhodamine-5-maleimide] CAS 154480-30-5

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Physical properties

Molecular weight	481.51
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	552
Emission (nm)	578

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

154480-30-5

Molecular weight

481.51

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.178

Extinction coefficient (cm ^-1 M ^-1)

90000

Excitation (nm)

552

Emission (nm)

578

Tetramethylrhodamine-5-maleimide is the purified single isomer of tetramethylrhodamine-5 (and 6)-maleimide mixed isomers. It is widely used for thiol modifications of peptides and proteins. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between the 5-isomer and 6-isomer might significantly affect some biological properties of the underlying conjugates. The 5-isomer is predominantly used to label peptides and proteins.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-TAMRA Maleimide [Tetramethylrhodamine-5-maleimide] *CAS 154480-30-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	207.68 µL	1.038 mL	2.077 mL	10.384 mL	20.768 mL
5 mM	41.536 µL	207.68 µL	415.36 µL	2.077 mL	4.154 mL
10 mM	20.768 µL	103.84 µL	207.68 µL	1.038 mL	2.077 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	552
Emission (nm)	578

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (260 nm)	Correction Factor (280 nm)
5(6)-TAMRA [5(6)-Carboxytetramethylrhodamine] CAS 98181-63-6	552	578	90000	0.32	0.178
5-TAMRA, SE [5-Carboxytetramethylrhodamine, succinimidyl ester] CAS#: 150810-68-7	552	578	90000	0.32	0.178
6-TAMRA Maleimide [Tetramethylrhodamine-6-maleimide] CAS 174568-68-4	552	578	90000	0.32	0.178
Fluorescein-5-maleimide CAS 75350-46-8	498	517	80000¹	0.32	0.35
Cyanine 5 maleimide [equivalent to Cy5® maleimide]	651	670	250000¹	0.02	0.03
5-TAMRA cadaverine	552	578	90000	0.32	0.178
5-TAMRA ethylenediamine	552	578	90000	0.32	0.178
5-TAMRA azide	552	578	90000	0.32	0.178
5-TAMRA alkyne	552	578	90000	0.32	0.178

Images

Figure 1. Chemical structure for 5-TAMRA Maleimide [Tetramethylrhodamine-5-maleimide] *CAS 154480-30-5*

References

View all 21 references: Citation Explorer

Severing of F-actin by yeast cofilin is pH-independent
Authors: Pavlov D, Muhlrad A, Cooper J, Wear M, Reisler E.
Journal: Cell Motil Cytoskeleton (2006): 533

Fluorometric measurements of intermolecular distances between the alpha and beta subunits of the Na+/K+-ATPase
Authors: Dempski RE, Hartung K, Friedrich T, Bamberg E.
Journal: J Biol Chem. (2006)

4-aminopyridine prevents the conformational changes associated with P/C-type inactivation in Shaker channels
Authors: Claydon TW, Vaid M, Rezazadeh S, Kehl SJ, Fedida D.
Journal: J Pharmacol Exp Ther. (2006)

Both rotor and stator subunits are necessary for efficient binding of F1 to F0 in functionally assembled Escherichia coli ATP synthase
Authors: Krebstakies T, Zimmermann B, Graber P, Altendorf K, Borsch M, Greie JC.
Journal: J Biol Chem (2005): 33338

The beta subunit of the Na+/K+-ATPase follows the conformational state of the holoenzyme
Authors: Dempski RE, Friedrich T, Bamberg E.
Journal: J Gen Physiol (2005): 505

Perturbation analysis of the voltage-sensitive conformational changes of the Na+/glucose cotransporter
Authors: Loo DD, Hirayama BA, Cha A, Bezanilla F, Wright EM.
Journal: J Gen Physiol (2005): 13

Cytochrome c conformations resolved by the photon counting histogram: watching the alkaline transition with single-molecule sensitivity
Authors: Perroud TD, Bokoch MP, Zare RN.
Journal: Proc Natl Acad Sci U S A (2005): 17570

Membrane topology of loop 13-14 of the Na+/glucose cotransporter (SGLT1): a SCAM and fluorescent labelling study
Authors: Gagnon DG, Holt A, Bourgeois F, Wallendorff B, Coady MJ, Lapointe JY.
Journal: Biochim Biophys Acta (2005): 173

Distances between tropomyosin sites across the muscle thin filament using luminescence resonance energy transfer: evidence for tropomyosin flexibility
Authors: Chen Y, Lehrer SS.
Journal: Biochemistry (2004): 11491

Crystal structure of monomeric actin in the ATP state. Structural basis of nucleotide-dependent actin dynamics
Authors: Graceffa P, Dominguez R.
Journal: J Biol Chem (2003): 34172