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5-TAMRA, SE [5-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-68-7*
The single TAMRA isomers are increasingly preferred for labeling peptides and nucleotides because they give better resolution in HPLC purification that is often required in the conjugation processes. 5-TAMRA, SE is predominantly used for labeling peptides and proteins while 6-TAMRA, SE is often used for labeling nucleotides and sequencing nucleic acids.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 5-TAMRA, SE [5-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-68-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 189.563 µL | 947.813 µL | 1.896 mL | 9.478 mL | 18.956 mL |
| 5 mM | 37.913 µL | 189.563 µL | 379.125 µL | 1.896 mL | 3.791 mL |
| 10 mM | 18.956 µL | 94.781 µL | 189.563 µL | 947.813 µL | 1.896 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
| 5(6)-TAMRA [5(6)-Carboxytetramethylrhodamine] *CAS 98181-63-6* | 552 | 578 | 90000 | 0.32 | 0.178 |
| 5-CR6G, SE [5-Carboxyrhodamine 6G, succinimidyl ester] *Single isomer* | 522 | 546 | 94000 | 0.24 | 0.214 |
| 5-ROX, SE [5-Carboxy-X-rhodamine, succinimidyl ester] *CAS 209734-74-7* | 578 | 604 | 82000 | - | 0.168 |
| 5-TAMRA Maleimide [Tetramethylrhodamine-5-maleimide] *CAS 154480-30-5* | 552 | 578 | 90000 | 0.32 | 0.178 |
| 6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-69-8* | 552 | 578 | 90000 | 0.32 | 0.178 |
| 5-FAM, SE [5-Carboxyfluorescein, succinimidyl ester] *CAS 92557-80-7* | 493 | 517 | 83000 | 0.32 | 0.178 |
| 5-TAMRA alkyne | 552 | 578 | 90000 | 0.32 | 0.178 |
| 5-TAMRA azide | 552 | 578 | 90000 | 0.32 | 0.178 |
| 5-TAMRA cadaverine | 552 | 578 | 90000 | 0.32 | 0.178 |
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Citations
View all 4 citations: Citation Explorer
Amikacin liposome inhalation suspension (ALIS) penetrates non-tuberculous mycobacterial biofilms and enhances amikacin uptake into macrophages
Authors: Zhang, Jimin and Leifer, Franziska and Rose, Sasha and Chun, Dung Yu and Thaisz, Jill and Herr, Tracey and Nashed, Mary and Joseph, Jayanthi and Perkins, Walter R and DiPetrillo, Keith
Journal: Frontiers in microbiology (2018)
Authors: Zhang, Jimin and Leifer, Franziska and Rose, Sasha and Chun, Dung Yu and Thaisz, Jill and Herr, Tracey and Nashed, Mary and Joseph, Jayanthi and Perkins, Walter R and DiPetrillo, Keith
Journal: Frontiers in microbiology (2018)
Design and Characterization of Two Bifunctional Cryptophane A-Based Host Molecules for Xenon Magnetic Resonance Imaging Applications
Authors: Rossella, Federica and Rose, Honor May and Witte, Christopher and Jayapaul, Jabadurai and Schröder, Leif
Journal: ChemPlusChem (2014): 1463--1471
Authors: Rossella, Federica and Rose, Honor May and Witte, Christopher and Jayapaul, Jabadurai and Schröder, Leif
Journal: ChemPlusChem (2014): 1463--1471
Human erythrocytes as drug carriers: loading efficiency and side effects of hypotonic dialysis, chlorpromazine treatment and fusion with liposomes
Authors: Favretto, ME and Cluitmans, JCA and Bosman, GJCGM and Brock, R
Journal: Journal of Controlled Release (2013): 343--351
Authors: Favretto, ME and Cluitmans, JCA and Bosman, GJCGM and Brock, R
Journal: Journal of Controlled Release (2013): 343--351
Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Preus, Søren and Wilhelmsson, L Marcus
Journal: ChemBioChem (2012): 1990--2001
Authors: Preus, Søren and Wilhelmsson, L Marcus
Journal: ChemBioChem (2012): 1990--2001
References
View all 96 references: Citation Explorer
Metal-enhanced fluorescence-based RNA sensing
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Journal: J Am Chem Soc (2006): 4206
Authors: Aslan K, Huang J, Wilson GM, Geddes CD.
Journal: J Am Chem Soc (2006): 4206
G Quadruplex-Based FRET Probes with the Thrombin-Binding Aptamer (TBA) Sequence Designed for the Efficient Fluorometric Detection of the Potassium Ion
Authors: Nagatoishi S, Nojima T, Galezowska E, Juskowiak B, Takenaka S.
Journal: Chembiochem (2006): 1730
Authors: Nagatoishi S, Nojima T, Galezowska E, Juskowiak B, Takenaka S.
Journal: Chembiochem (2006): 1730
A targeted protease substrate for a quantitative determination of protease activities in the endolysosomal pathway
Authors: Fischer R, Bachle D, Fotin-Mleczek M, Jung G, Kalbacher H, Brock R.
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Authors: Fischer R, Bachle D, Fotin-Mleczek M, Jung G, Kalbacher H, Brock R.
Journal: Chembiochem (2006): 1428
Electrostatic-gated transport in chemically modified glass nanopore electrodes
Authors: Wang G, Zhang B, Wayment JR, Harris JM, White HS.
Journal: J Am Chem Soc (2006): 7679
Authors: Wang G, Zhang B, Wayment JR, Harris JM, White HS.
Journal: J Am Chem Soc (2006): 7679
Fluorescence anisotropy and FRET studies of G-quadruplex formation in presence of different cations
Authors: Juskowiak B, Galezowska E, Zawadzka A, Gluszynska A, Takenaka S.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 835
Authors: Juskowiak B, Galezowska E, Zawadzka A, Gluszynska A, Takenaka S.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 835
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