5-TAMRA, SE [5-Carboxytetramethylrhodamine, succinimidyl ester] CAS#: 150810-68-7

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Physical properties

Molecular weight	527.53
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	552
Emission (nm)	578

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

150810-68-7

Molecular weight

527.53

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.178

Extinction coefficient (cm ^-1 M ^-1)

90000

Excitation (nm)

552

Emission (nm)

578

The single TAMRA isomers are increasingly preferred for labeling peptides and nucleotides because they give better resolution in HPLC purification that is often required in the conjugation processes. 5-TAMRA, SE is predominantly used for labeling peptides and proteins while 6-TAMRA, SE is often used for labeling nucleotides and sequencing nucleic acids.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-TAMRA, SE [5-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-68-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	189.563 µL	947.813 µL	1.896 mL	9.478 mL	18.956 mL
5 mM	37.913 µL	189.563 µL	379.125 µL	1.896 mL	3.791 mL
10 mM	18.956 µL	94.781 µL	189.563 µL	947.813 µL	1.896 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	552
Emission (nm)	578

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (260 nm)	Correction Factor (280 nm)
5-CR6G, SE [5-Carboxyrhodamine 6G, succinimidyl ester] Single isomer	522	546	94000	0.24	0.214
5(6)-TAMRA [5(6)-Carboxytetramethylrhodamine] CAS 98181-63-6	552	578	90000	0.32	0.178
6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] CAS#: 150810-69-8	552	578	90000	0.32	0.178
5-ROX, SE [5-Carboxy-X-rhodamine, succinimidyl ester] CAS 209734-74-7	578	604	82000	-	0.168
5-TAMRA Maleimide [Tetramethylrhodamine-5-maleimide] CAS 154480-30-5	552	578	90000	0.32	0.178
5-FAM, SE [5-Carboxyfluorescein, succinimidyl ester] CAS 92557-80-7	493	517	83000	0.32	0.178
5-TAMRA cadaverine	552	578	90000	0.32	0.178
5-TAMRA ethylenediamine	552	578	90000	0.32	0.178
5-TAMRA azide	552	578	90000	0.32	0.178
5-TAMRA alkyne	552	578	90000	0.32	0.178
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Images

Figure 1. Chemical structure for 5-TAMRA, SE [5-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-68-7*

Citations

View all 4 citations: Citation Explorer

Amikacin liposome inhalation suspension (ALIS) penetrates non-tuberculous mycobacterial biofilms and enhances amikacin uptake into macrophages
Authors: Zhang, Jimin and Leifer, Franziska and Rose, Sasha and Chun, Dung Yu and Thaisz, Jill and Herr, Tracey and Nashed, Mary and Joseph, Jayanthi and Perkins, Walter R and DiPetrillo, Keith
Journal: Frontiers in microbiology (2018)

Design and Characterization of Two Bifunctional Cryptophane A-Based Host Molecules for Xenon Magnetic Resonance Imaging Applications
Authors: Rossella, Federica and Rose, Honor May and Witte, Christopher and Jayapaul, Jabadurai and Schröder, Leif
Journal: ChemPlusChem (2014): 1463--1471

Human erythrocytes as drug carriers: loading efficiency and side effects of hypotonic dialysis, chlorpromazine treatment and fusion with liposomes
Authors: Favretto, ME and Cluitmans, JCA and Bosman, GJCGM and Brock, R
Journal: Journal of Controlled Release (2013): 343--351

Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Preus, Søren and Wilhelmsson, L Marcus
Journal: ChemBioChem (2012): 1990--2001

References

View all 96 references: Citation Explorer

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A fluorescence polarization assay for screening inhibitors against the ribonuclease H activity of HIV-1 reverse transcriptase
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High efficiency micellar electrokinetic chromatography of hydrophobic analytes on poly(dimethylsiloxane) microchips
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Journal: Analyst (2006): 194

Metal-enhanced fluorescence-based RNA sensing
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Fluorescence anisotropy and FRET studies of G-quadruplex formation in presence of different cations
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Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 835

Electrostatic-gated transport in chemically modified glass nanopore electrodes
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Journal: J Am Chem Soc (2006): 7679

A targeted protease substrate for a quantitative determination of protease activities in the endolysosomal pathway
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Journal: Chembiochem (2006): 1428

G Quadruplex-Based FRET Probes with the Thrombin-Binding Aptamer (TBA) Sequence Designed for the Efficient Fluorometric Detection of the Potassium Ion
Authors: Nagatoishi S, Nojima T, Galezowska E, Juskowiak B, Takenaka S.
Journal: Chembiochem (2006): 1730

Fluorescence properties of fluorescein, tetramethylrhodamine and Texas Red linked to a DNA aptamer
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