5-VIC phosphoramidite
| Overview |  SDSProtocol |
See also: Cell Structures and Organelles, Nucleus, DNA and RNA Quantitation, Physiological Probes, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), PCR Detection of Viral DNA/RNA
Molecular weight 1023.38 | Excitation (nm) 526 | Emission (nm) 543 |
VIC is a fluorescent dye that was originally developed by Applied Biosystems (now, Thermofisher). VIC emits in the green-yellow part of the visible spectrum. It is used to fluorescently label oligonucleotides at the 5’-end (for use as probes in a variety of real-time PCR, hybridization, and fluorescence-based genetic analysis applications). VIC phosphoramidite is the most convenient building block to incorporate VIC tag into an oligonucleotide.
Calculators
Common stock solution preparation
Table 1. Volume of MeCN needed to reconstitute specific mass of 5-VIC phosphoramidite to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 97.715 µL | 488.577 µL | 977.154 µL | 4.886 mL | 9.772 mL |
| 5 mM | 19.543 µL | 97.715 µL | 195.431 µL | 977.154 µL | 1.954 mL |
| 10 mM | 9.772 µL | 48.858 µL | 97.715 µL | 488.577 µL | 977.154 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Images
Figure 1. Chemical structure for 5-VIC phosphoramidite.