6-CR6G, SE [6-Carboxyrhodamine 6G, succinimidyl ester] Single isomer

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	555.59
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.24
Correction Factor (280 nm)	0.214
Extinction coefficient (cm ^-1 M ^-1)	94000
Excitation (nm)	522
Emission (nm)	546

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

555.59

Correction Factor (260 nm)

0.24

Correction Factor (280 nm)

0.214

Extinction coefficient (cm ^-1 M ^-1)

94000

Excitation (nm)

522

Emission (nm)

546

6-CR6G, SE is amine-reactive derivative of 6-CR6G. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-CR6G and 6-CR6G might significantly affect some biological properties of the underlying conjugates.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-CR6G, SE [6-Carboxyrhodamine 6G, succinimidyl ester] *Single isomer* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	179.989 µL	899.944 µL	1.8 mL	8.999 mL	17.999 mL
5 mM	35.998 µL	179.989 µL	359.978 µL	1.8 mL	3.6 mL
10 mM	17.999 µL	89.994 µL	179.989 µL	899.944 µL	1.8 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.24
Correction Factor (280 nm)	0.214
Extinction coefficient (cm ^-1 M ^-1)	94000
Excitation (nm)	522
Emission (nm)	546

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
5(6)-CR6G [5-(and 6)-Carboxyrhodamine 6G]	522	546	94000	0.214
5-CR6G, SE [5-Carboxyrhodamine 6G, succinimidyl ester] Single isomer	522	546	94000	0.214
6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] CAS#: 150810-69-8	552	578	90000	0.178
6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] CAS#: 216699-36-4	578	604	82000	0.168
6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] CAS 92557-81-8	493	517	83000	0.178
6-HEX, SE [6-Carboxy-2',4,4',5',7,7'-hexachlorofluorescein, succinimidyl ester] Single Isomer	533	559	73000	0.15
6-JOE, SE [6-Carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein, succinimidyl ester] CAS#: 113394-23-3	520	545	75000¹	-
6-TET, SE [6-Carboxy-2',4,7',7-tetrachlorofluorescein, succinimidyl ester]	521	542	73000	0.13
6-ROXtra™ SE Superior 6-ROX Replacement	578	595	-	-
6-VIC, SE [6-VIC NHS ester]	526	543	-	-
6-NED, SE [6-NED NHS ester]	545	567	74000¹	-
Show More (2)

Images

Figure 1. Chemical structure for 6-CR6G, SE [6-Carboxyrhodamine 6G, succinimidyl ester] *Single isomer*

Citations

View all 2 citations: Citation Explorer

Rotational motion of rhodamine 6G tethered to actin through oligo (ethylene glycol) linkers studied by frequency-domain fluorescence anisotropy
Authors: Wazawa, Tetsuichi and Morimoto, Nobuyuki and Nagai, Takeharu and Suzuki, Makoto
Journal: Biophysics and physicobiology (2015): 87--102

References

View all 6 references: Citation Explorer

Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926

High-power blue/UV light-emitting diodes as excitation sources for sensitive detection
Authors: Kuo JS, Kuyper CL, Allen PB, Fiorini GS, Chiu DT.
Journal: Electrophoresis (2004): 3796

Modifying the adsorption behavior of polyamidoamine dendrimers at silica surfaces investigated by total internal reflection fluorescence correlation spectroscopy
Authors: McCain KS, Schluesche P, Harris JM.
Journal: Anal Chem (2004): 930

Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant
Authors: Arezi B, Hansen CJ, Hogrefe HH.
Journal: J Mol Biol (2002): 719

Fluorescence resonance energy transfer (FRET) and competing processes in donor-acceptor substituted DNA strands: a comparative study of ensemble and single-molecule data
Authors: Dietrich A, Buschmann V, Muller C, Sauer M.
Journal: J Biotechnol (2002): 211

Optimization of spectroscopic and electrophoretic properties of energy transfer primers
Authors: Hung SC, Mathies RA, Glazer AN.
Journal: Anal Biochem (1997): 78