Name: 6-dR110 [6-Carboxy-4,7-dichlororhodamine 110]
Brand: AAT Bioquest
SKU: 316
Price: 5798 USD
Availability: InStock

6-dR110

6-Carboxy-4,7-dichlororhodamine 110

Compared to fluorescein labeling reagents such as FITC and FAM, 6-dR110 [6-carboxy-4,7-dichlororhodamine 110] reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength. They are photostable alternative reagents superior to FITC and FAM. 5-carboxy-4,7-dichlororhodamine 110 is one of the four critical 4,7-dichloro-rhodamine dyes used as the BigDye terminators for DNA sequencing in combination with d6G, dTMR and dROX. ABI PRISM® BigDye™ Terminator Cycle Sequencing is the classic method for DNA sequencing.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
316	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-dR110 [6-Carboxy-4,7-dichlororhodamine 110] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	183.678 µL	918.392 µL	1.837 mL	9.184 mL	18.368 mL
5 mM	36.736 µL	183.678 µL	367.357 µL	1.837 mL	3.674 mL
10 mM	18.368 µL	91.839 µL	183.678 µL	918.392 µL	1.837 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	544.43 g/mol	=	mL	x	mM	=	mmol

References

View all 6 references: Citation Explorer

Design and synthesis of fluorescence-labeled nucleotide with a cleavable azo linker for DNA sequencing

Authors:

Tan, L.; Liu, Y.; Yang, Q.; Li, X.; Wu, X. Y.; Gong, B.; Shen, Y. M.; Shao, Z.

Journal:

Chem Commun (Camb) (2016): 954-7

Synthesis of novel tyrosinyl FRET cassettes, terminators, and their potential use in DNA sequencing

Authors:

Sudhakar Rao, T.; Zhang, W.; Xiao, H.; Flick, P.; Kumar, S.; Nampalli, S.

Journal:

Nucleosides Nucleotides Nucleic Acids (2003): 1443-5

Design strategies and performance of custom DNA sequencing primers

Authors:

Buck, G. A.; Fox, J. W.; Gunthorpe, M.; Hager, K. M.; Naeve, C. W.; Pon, R. T.; Adams, P. S.; Rush, J.

Journal:

Biotechniques (1999): 528-36

Improved DNA sequencing accuracy and detection of heterozygous alleles using manganese citrate and different fluorescent dye terminators

Authors:

Korch, C.; Drabkin, H.

Journal:

Genome Res (1999): 588-95

Peak height pattern in dichloro-rhodamine and energy transfer dye terminator sequencing

Authors:

Zakeri, H.; Amparo, G.; Chen, S. M.; Spurgeon, S.; Kwok, P. Y.

Journal:

Biotechniques (1998): 406-10, 412-4

Physical properties

Molecular weight	544.43
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 6, 2025