Calculator
6-HEX acid
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 582.97 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (280 nm) | 0.15 |
| Extinction coefficient (cm -1 M -1) | 73000 |
| Excitation (nm) | 533 |
| Emission (nm) | 559 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
| 6-HEX azide |
| 6-HEX alkyne |
| Overview |  SDSProtocol |
Molecular weight 582.97 | Correction Factor (280 nm) 0.15 | Extinction coefficient (cm -1 M -1) 73000 | Excitation (nm) 533 | Emission (nm) 559 |
6-HEX is a fluorophore that is widely used in nucleic acid sequencing and related research. 6-HEX acid is the most economic form to make a conjugate with amino-containing biomolecules.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 6-HEX acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 171.535 µL | 857.677 µL | 1.715 mL | 8.577 mL | 17.154 mL |
| 5 mM | 34.307 µL | 171.535 µL | 343.071 µL | 1.715 mL | 3.431 mL |
| 10 mM | 17.154 µL | 85.768 µL | 171.535 µL | 857.677 µL | 1.715 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (280 nm) | 0.15 |
| Extinction coefficient (cm -1 M -1) | 73000 |
| Excitation (nm) | 533 |
| Emission (nm) | 559 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| 6-HEX, SE [6-Carboxy-2',4,4',5',7,7'-hexachlorofluorescein, succinimidyl ester] *Single Isomer* | 533 | 559 | 73000 | 0.15 |
| 6-HEX phosphoramidite [5'-Hexachlorofluorescein phosphoramidite] | 533 | 559 | 73000 | 0.15 |
| 6-OG488 acid [equivalent to Oregon Green® 488 carboxylic acid, 6-isomer] | 498 | 526 | 76000 | 0.12 |
| 6-ROXtra™ acid | 578 | 595 | - | - |
| 6-JOE acid | 520 | 545 | 750001 | - |
| 6-TET Acid | 521 | 542 | 73000 | 0.13 |
Images
Figure 1. 6-HEX is a fluorophore that is widely used in nucleic acid sequencing and related research. 6-HEX acid is the most economic form to make a conjugate with amino-containing biomolecules.