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6-OG488 acid [equivalent to Oregon Green® 488 carboxylic acid, 6-isomer]
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 412.30 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (260 nm) | 0.31 |
| Correction Factor (280 nm) | 0.12 |
| Extinction coefficient (cm -1 M -1) | 76000 |
| Excitation (nm) | 498 |
| Emission (nm) | 526 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
See also: Fluoresceins
CAS 195136-53-9 | Molecular weight 412.30 | Correction Factor (260 nm) 0.31 | Correction Factor (280 nm) 0.12 | Extinction coefficient (cm -1 M -1) 76000 | Excitation (nm) 498 | Emission (nm) 526 |
6-OG488 acid is the same molecule to Oregon Green® 488 carboxylic acid, 6-isomer (Oregon Green® is the Trademark of Invitrogen). This fluorinated analog of fluorescein has higher photostability and a lower pKa ~ 4.7 while 6-FAM has pKa ~6.4. The decreased pKa of 6-OG488 makes its fluorescence essentially pH insensitive in the physiological pH range. This amine-reactive 6-OG488 can be used to make bright green fluorescent bioconjugates with excitation/emission maxima ~496/524 nm. It is an excellent replacement to 6-FITC that has severe pH dependence and low photostability.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 6-OG488 acid [equivalent to Oregon Green® 488 carboxylic acid, 6-isomer] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 242.542 µL | 1.213 mL | 2.425 mL | 12.127 mL | 24.254 mL |
| 5 mM | 48.508 µL | 242.542 µL | 485.084 µL | 2.425 mL | 4.851 mL |
| 10 mM | 24.254 µL | 121.271 µL | 242.542 µL | 1.213 mL | 2.425 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.31 |
| Correction Factor (280 nm) | 0.12 |
| Extinction coefficient (cm -1 M -1) | 76000 |
| Excitation (nm) | 498 |
| Emission (nm) | 526 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| 5-OG488 acid [equivalent to Oregon Green® 488 carboxylic acid, 5-isomer] | 498 | 526 | 76000 | 0.12 |
| 6-ROXtra™ acid | 578 | 595 | - | - |
| 6-OG488 Azide | 498 | 526 | 76000 | 0.12 |
| 6-JOE acid | 520 | 545 | 750001 | - |
| 6-HEX acid | 533 | 559 | 73000 | 0.15 |
| 6-TET Acid | 521 | 542 | 73000 | 0.13 |
Images
Figure 1. Chemical structure for 6-OG488 acid [equivalent to Oregon Green® 488 carboxylic acid, 6-isomer]
References
View all 14 references: Citation Explorer
Biodegradable polymersomes as carriers and release systems for paclitaxel using Oregon Green(R) 488 labeled paclitaxel as a model compound
Authors: Lee JS, Feijen J.
Journal: J Control Release (2012): 312
Authors: Lee JS, Feijen J.
Journal: J Control Release (2012): 312
Microscopic imaging of intracellular calcium in live cells using lifetime-based ratiometric measurements of Oregon Green BAPTA-1
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377
Trans-scleral permeability of Oregon green 488
Authors: Lee SJ, Kim SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: J Ocul Pharmacol Ther (2008): 579
Authors: Lee SJ, Kim SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: J Ocul Pharmacol Ther (2008): 579
Pharmacokinetics of intraocular drug delivery of Oregon green 488-labeled triamcinolone by subtenon injection using ocular fluorophotometry in rabbit eyes
Authors: Lee SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: Invest Ophthalmol Vis Sci (2008): 4506
Authors: Lee SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: Invest Ophthalmol Vis Sci (2008): 4506
A mismatch-selective bifunctional rhodium-Oregon Green conjugate: a fluorescent probe for mismatched DNA
Authors: Zeglis BM, Barton JK.
Journal: J Am Chem Soc (2006): 5654
Authors: Zeglis BM, Barton JK.
Journal: J Am Chem Soc (2006): 5654
The Pennsylvania Green Fluorophore: a hybrid of Oregon Green and Tokyo Green for the construction of hydrophobic and pH-insensitive molecular probes
Authors: Mottram LF, Boonyarattanakalin S, Kovel RE, Peterson BR.
Journal: Org Lett (2006): 581
Authors: Mottram LF, Boonyarattanakalin S, Kovel RE, Peterson BR.
Journal: Org Lett (2006): 581
Collagen-binding adhesion protein 35-Oregon Green 488
Authors: Cheng KT, Van Z and voort M., undefined
Journal: In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD). (2004)
Authors: Cheng KT, Van Z and voort M., undefined
Journal: In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD). (2004)
Cetuximab-Oregon Green 488
Authors: , undefined
Journal: In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD) (2004)
Authors: , undefined
Journal: In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD) (2004)
Alexa and Oregon Green dyes as fluorescence anisotropy probes for measuring protein-protein and protein-nucleic acid interactions
Authors: Rusinova E, Tretyachenko-Ladokhina V, Vele OE, Senear DF, Alex and er Ross JB., undefined
Journal: Anal Biochem (2002): 18
Authors: Rusinova E, Tretyachenko-Ladokhina V, Vele OE, Senear DF, Alex and er Ross JB., undefined
Journal: Anal Biochem (2002): 18
Imaging of changes in sarcoplasmic reticulum [Ca(2+)] using Oregon Green BAPTA 5N and confocal laser scanning microscopy
Authors: White C, McGeown G.
Journal: Cell Calcium (2002): 151
Authors: White C, McGeown G.
Journal: Cell Calcium (2002): 151
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