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6-OG488 succinimidyl ester [equivalent to Oregon Green® 488 carboxylic acid, succinimidyl ester, 6-isomer ]
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Application notes
6-OG488 succinimidyl ester is the same molecule to Oregon Green® 488 carboxylic acid, succinimidyl ester, 6-isomer (Invitrogen #O6185, Oregon Green® is the Trademark of Invitrogen). This fluorinated analog of fluorescein has higher photostability and a lower pKa ~ 4.7 while 6-FAM has pKa ~6.4. The decreased pKa of 6-OG488 makes its fluorescence essentially pH insensitive in the physiological pH range. This amine-reactive 6-OG488 succinimidyl ester can be used to make bright green fluorescent bioconjugates with excitation/emission maxima ~496/524 nm. It is an excellent replacement to 6-FITC that has severe pH dependence and low photostability.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 6-OG488 succinimidyl ester [equivalent to Oregon Green® 488 carboxylic acid, succinimidyl ester, 6-isomer ] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 196.321 µL | 981.605 µL | 1.963 mL | 9.816 mL | 19.632 mL |
| 5 mM | 39.264 µL | 196.321 µL | 392.642 µL | 1.963 mL | 3.926 mL |
| 10 mM | 19.632 µL | 98.16 µL | 196.321 µL | 981.605 µL | 1.963 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
| 5-OG488 succinimidyl ester [equivalent to Oregon Green® 488 carboxylic acid, succinimidyl ester, 5-isomer ] | 498 | 526 | 76000 | 0.31 | 0.12 |
References
View all 14 references: Citation Explorer
Biodegradable polymersomes as carriers and release systems for paclitaxel using Oregon Green(R) 488 labeled paclitaxel as a model compound
Authors: Lee JS, Feijen J.
Journal: J Control Release (2012): 312
Authors: Lee JS, Feijen J.
Journal: J Control Release (2012): 312
Microscopic imaging of intracellular calcium in live cells using lifetime-based ratiometric measurements of Oregon Green BAPTA-1
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377
Pharmacokinetics of intraocular drug delivery of Oregon green 488-labeled triamcinolone by subtenon injection using ocular fluorophotometry in rabbit eyes
Authors: Lee SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: Invest Ophthalmol Vis Sci (2008): 4506
Authors: Lee SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: Invest Ophthalmol Vis Sci (2008): 4506
Trans-scleral permeability of Oregon green 488
Authors: Lee SJ, Kim SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: J Ocul Pharmacol Ther (2008): 579
Authors: Lee SJ, Kim SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: J Ocul Pharmacol Ther (2008): 579
The Pennsylvania Green Fluorophore: a hybrid of Oregon Green and Tokyo Green for the construction of hydrophobic and pH-insensitive molecular probes
Authors: Mottram LF, Boonyarattanakalin S, Kovel RE, Peterson BR.
Journal: Org Lett (2006): 581
Authors: Mottram LF, Boonyarattanakalin S, Kovel RE, Peterson BR.
Journal: Org Lett (2006): 581