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6-ROX-AHA, SE

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Physical properties
Molecular weight744.83
SolventDMSO
Spectral properties
Correction Factor (280 nm)0.168
Extinction coefficient (cm -1 M -1)82000
Excitation (nm)578
Emission (nm)604
Quantum yield0.941
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
744.83
Correction Factor (280 nm)
0.168
Extinction coefficient (cm -1 M -1)
82000
Excitation (nm)
578
Emission (nm)
604
Quantum yield
0.941
6-ROX is predominately used for labeling oligonucleotides and sequencing nucleic acids. However, 6-ROX SE is very unstable compared to other rhodamine dyes. 6-ROX AHA has been developed to improve the stability of 6-ROX SE. The bioconjugates derived from 6-ROX AHA SE give the same spectral properties to those of the bioconjugates prepared from 6-ROX SE. In most cases 6-ROX AHA SE give higher conjugation yields than 6-ROX SE does, and in some cases the conjugates of 6-ROX AHA are brighter than those of 6-ROX probably due to the additional spacer between the ROX fluorophore and biomolecules to be labeled.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-ROX-AHA, SE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM134.259 µL671.294 µL1.343 mL6.713 mL13.426 mL
5 mM26.852 µL134.259 µL268.518 µL1.343 mL2.685 mL
10 mM13.426 µL67.129 µL134.259 µL671.294 µL1.343 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Correction Factor (280 nm)0.168
Extinction coefficient (cm -1 M -1)82000
Excitation (nm)578
Emission (nm)604
Quantum yield0.941

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)
5(6)-ROX, SE [5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester] *Mixed isomers*578604820000.9410.168

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References


View all 11 references: Citation Explorer
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence
Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834
Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488
Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412
Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
Authors: Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: Invest Ophthalmol Vis Sci (2003): 1312
HLA-DRB fluorotyping by dark quenching and automated analysis
Authors: Slateva K, Elsner HA, Albis-Camps M, Blasczyk R.
Journal: Tissue Antigens (2001): 250
Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction--amplified short tandem repeats during denaturing capillary electrophoresis
Authors: Hahn M, Wilhelm J, Pingoud A.
Journal: Electrophoresis (2001): 2691
Design, synthesis, and spectroscopic properties of peptide-bridged fluorescence energy-transfer cassettes
Authors: Li Y, Glazer AN.
Journal: Bioconjug Chem (1999): 241
Differential display with carboxy-X-rhodamine-labeled primers and the selection of differentially amplified cDNA fragments without cloning
Authors: Yoshikawa Y, Mukai H, Asada K, Hino F, Kato I.
Journal: Anal Biochem (1998): 82
Comparison of fluorescence energy transfer primers with different donor-acceptor dye combinations
Authors: Hung SC, Mathies RA, Glazer AN.
Journal: Anal Biochem (1998): 32