6-ROX-AHA, SE
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 744.83 |
Solvent | DMSO |
Spectral properties
Correction Factor (280 nm) | 0.168 |
Extinction coefficient (cm -1 M -1) | 82000 |
Excitation (nm) | 578 |
Emission (nm) | 604 |
Quantum yield | 0.941 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
Molecular weight 744.83 | Correction Factor (280 nm) 0.168 | Extinction coefficient (cm -1 M -1) 82000 | Excitation (nm) 578 | Emission (nm) 604 | Quantum yield 0.941 |
6-ROX is predominately used for labeling oligonucleotides and sequencing nucleic acids. However, 6-ROX SE is very unstable compared to other rhodamine dyes. 6-ROX AHA has been developed to improve the stability of 6-ROX SE. The bioconjugates derived from 6-ROX AHA SE give the same spectral properties to those of the bioconjugates prepared from 6-ROX SE. In most cases 6-ROX AHA SE give higher conjugation yields than 6-ROX SE does, and in some cases the conjugates of 6-ROX AHA are brighter than those of 6-ROX probably due to the additional spacer between the ROX fluorophore and biomolecules to be labeled.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 6-ROX-AHA, SE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 134.259 µL | 671.294 µL | 1.343 mL | 6.713 mL | 13.426 mL |
5 mM | 26.852 µL | 134.259 µL | 268.518 µL | 1.343 mL | 2.685 mL |
10 mM | 13.426 µL | 67.129 µL | 134.259 µL | 671.294 µL | 1.343 mL |
Molarity calculator
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Spectrum
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Spectral properties
Correction Factor (280 nm) | 0.168 |
Extinction coefficient (cm -1 M -1) | 82000 |
Excitation (nm) | 578 |
Emission (nm) | 604 |
Quantum yield | 0.941 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (280 nm) |
5(6)-ROX, SE [5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester] *Mixed isomers* | 578 | 604 | 82000 | 0.941 | 0.168 |
References
View all 11 references: Citation Explorer
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence
Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834
Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834
Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488
Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412
Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
Authors: Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: Invest Ophthalmol Vis Sci (2003): 1312
Authors: Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: Invest Ophthalmol Vis Sci (2003): 1312
HLA-DRB fluorotyping by dark quenching and automated analysis
Authors: Slateva K, Elsner HA, Albis-Camps M, Blasczyk R.
Journal: Tissue Antigens (2001): 250
Authors: Slateva K, Elsner HA, Albis-Camps M, Blasczyk R.
Journal: Tissue Antigens (2001): 250
Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction--amplified short tandem repeats during denaturing capillary electrophoresis
Authors: Hahn M, Wilhelm J, Pingoud A.
Journal: Electrophoresis (2001): 2691
Authors: Hahn M, Wilhelm J, Pingoud A.
Journal: Electrophoresis (2001): 2691
Design, synthesis, and spectroscopic properties of peptide-bridged fluorescence energy-transfer cassettes
Authors: Li Y, Glazer AN.
Journal: Bioconjug Chem (1999): 241
Authors: Li Y, Glazer AN.
Journal: Bioconjug Chem (1999): 241
Differential display with carboxy-X-rhodamine-labeled primers and the selection of differentially amplified cDNA fragments without cloning
Authors: Yoshikawa Y, Mukai H, Asada K, Hino F, Kato I.
Journal: Anal Biochem (1998): 82
Authors: Yoshikawa Y, Mukai H, Asada K, Hino F, Kato I.
Journal: Anal Biochem (1998): 82
Comparison of fluorescence energy transfer primers with different donor-acceptor dye combinations
Authors: Hung SC, Mathies RA, Glazer AN.
Journal: Anal Biochem (1998): 32
Authors: Hung SC, Mathies RA, Glazer AN.
Journal: Anal Biochem (1998): 32