6-ROX C2 Maleimide

6-ROX C2 maleimide is an excellent thiol-reactive ROX probe that can be used to develop new ROX bioconjugates. It selectively reacts with thiol groups of peptides, proteins and other biological molecules (such as thiol-modified oligonucleotides and small thiol-containing organic molecules). 6-ROX C2 maleimide has the spectral properties identical to those of 6-ROX conjugates, thus all the instrument settings of the 6-ROX probes would be applicable to the applications of 6-ROX C2 maleimide conjugates. Compared to the commonly used 6-ROX acid and 6-ROX SE, 6-ROX C2 maleimide is much more stable.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
426	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-ROX C2 Maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	152.27 µL	761.348 µL	1.523 mL	7.613 mL	15.227 mL
5 mM	30.454 µL	152.27 µL	304.539 µL	1.523 mL	3.045 mL
10 mM	15.227 µL	76.135 µL	152.27 µL	761.348 µL	1.523 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	656.73 g/mol	=	mL	x	mM	=	mmol

References

View all 11 references: Citation Explorer

Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides

Authors:

Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.

Journal:

Proc Natl Acad Sci U S A (2005): 5926

Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis

Authors:

Faulds K, Smith WE, Graham D.

Journal:

Anal Chem (2004): 412

Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry

Authors:

Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.

Journal:

Proc Natl Acad Sci U S A (2004): 5488

Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence

Authors:

Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.

Journal:

Langmuir (2004): 6834

Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport

Authors:

Yang JH, Basinger SF, Gross RL, Wu SM.

Journal:

Invest Ophthalmol Vis Sci (2003): 1312

Physical properties

Molecular weight	656.73
Solvent	DMSO

Spectral properties

Correction factor (280 nm)	0.168
Extinction coefficient (cm ^-1 M ^-1)	82000
Excitation (nm)	578
Emission (nm)	604
Quantum yield	0.94 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 11, 2025