6-ROX glycine *25 uM fluorescence reference solution for PCR reactions*

6-ROX glycine

25 uM fluorescence reference solution for PCR reactions

6-ROX is predominately used as a reference dye for performing PCR detections. However, 6-ROX is very unstable compared to other rhodamine dyes. 6-ROX glycine has improved stability. The 6-ROX glycine has the similar spectral properties to those of 6-ROX. This 6-ROX dye is 25 uM solution in 20 mM Tris (pH 8.4), 0.1 mM EDTA and 0.01% Tween® 20.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
395	5 ml	Price

References

View all 11 references: Citation Explorer

Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides

Authors:

Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.

Journal:

Proc Natl Acad Sci U S A (2005): 5926

Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis

Authors:

Faulds K, Smith WE, Graham D.

Journal:

Anal Chem (2004): 412

Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry

Authors:

Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.

Journal:

Proc Natl Acad Sci U S A (2004): 5488

Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence

Authors:

Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.

Journal:

Langmuir (2004): 6834

Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport

Authors:

Yang JH, Basinger SF, Gross RL, Wu SM.

Journal:

Invest Ophthalmol Vis Sci (2003): 1312

Physical properties

Molecular weight	591.65
Solvent	Water

Spectral properties

Correction factor (280 nm)	0.168
Extinction coefficient (cm ^-1 M ^-1)	82000
Excitation (nm)	578
Emission (nm)	604
Quantum yield	0.94 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on September 24, 2025