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6-ROX glycine *25 uM fluorescence reference solution for PCR reactions*

6-ROX is predominately used as a reference dye for performing PCR detections. However, 6-ROX is very unstable compared to other rhodamine dyes. 6-ROX glycine has improved stability. The 6-ROX glycine has the similar spectral properties to those of 6-ROX. This 6-ROX dye is 25 uM solution in 20 mM Tris (pH 8.4), 0.1 mM EDTA and 0.01% Tween® 20.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)
5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] *CAS 198978-94-8*578604820000.9410.168
6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS#: 216699-36-4*578604820000.9410.168
5-ROX glycine *Fluorescence reference standard for PCR reactions*578604820000.9410.168
6-ROX azide578604820000.9410.168
6-ROX alkyne578604820000.9410.168

References

View all 11 references: Citation Explorer
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412
Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488
Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence
Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834
Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
Authors: Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: Invest Ophthalmol Vis Sci (2003): 1312
Page updated on December 11, 2024

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Physical properties

Molecular weight

591.65

Solvent

Water

Spectral properties

Correction Factor (280 nm)

0.168

Extinction coefficient (cm -1 M -1)

82000

Excitation (nm)

578

Emission (nm)

604

Quantum yield

0.941

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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