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6-ROX glycine *25 uM fluorescence reference solution for PCR reactions*

Chemical structure for 6-ROX glycine *25 uM fluorescence reference solution for PCR reactions*
Chemical structure for 6-ROX glycine *25 uM fluorescence reference solution for PCR reactions*
Ordering information
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Catalog Number395
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight591.65
SolventWater
Spectral properties
Correction Factor (280 nm)0.168
Extinction coefficient (cm -1 M -1)82000
Excitation (nm)578
Emission (nm)604
Quantum yield0.941
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
591.65
Correction Factor (280 nm)
0.168
Extinction coefficient (cm -1 M -1)
82000
Excitation (nm)
578
Emission (nm)
604
Quantum yield
0.941
6-ROX is predominately used as a reference dye for performing PCR detections. However, 6-ROX is very unstable compared to other rhodamine dyes. 6-ROX glycine has improved stability. The 6-ROX glycine has the similar spectral properties to those of 6-ROX. This 6-ROX dye is 25 uM solution in 20 mM Tris (pH 8.4), 0.1 mM EDTA and 0.01% Tween® 20.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of 6-ROX glycine *25 uM fluorescence reference solution for PCR reactions* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM169.019 µL845.094 µL1.69 mL8.451 mL16.902 mL
5 mM33.804 µL169.019 µL338.038 µL1.69 mL3.38 mL
10 mM16.902 µL84.509 µL169.019 µL845.094 µL1.69 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectrum


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spectrum

Spectral properties

Correction Factor (280 nm)0.168
Extinction coefficient (cm -1 M -1)82000
Excitation (nm)578
Emission (nm)604
Quantum yield0.941

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)
5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] *CAS 198978-94-8*578604820000.9410.168
6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS#: 216699-36-4*578604820000.9410.168
5-ROX glycine *Fluorescence reference standard for PCR reactions*578604820000.9410.168
6-ROX azide578604820000.9410.168
6-ROX alkyne578604820000.9410.168

References


View all 11 references: Citation Explorer
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence
Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834
Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488
Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412
Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
Authors: Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: Invest Ophthalmol Vis Sci (2003): 1312
HLA-DRB fluorotyping by dark quenching and automated analysis
Authors: Slateva K, Elsner HA, Albis-Camps M, Blasczyk R.
Journal: Tissue Antigens (2001): 250
Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction--amplified short tandem repeats during denaturing capillary electrophoresis
Authors: Hahn M, Wilhelm J, Pingoud A.
Journal: Electrophoresis (2001): 2691
Design, synthesis, and spectroscopic properties of peptide-bridged fluorescence energy-transfer cassettes
Authors: Li Y, Glazer AN.
Journal: Bioconjug Chem (1999): 241
Differential display with carboxy-X-rhodamine-labeled primers and the selection of differentially amplified cDNA fragments without cloning
Authors: Yoshikawa Y, Mukai H, Asada K, Hino F, Kato I.
Journal: Anal Biochem (1998): 82
Comparison of fluorescence energy transfer primers with different donor-acceptor dye combinations
Authors: Hung SC, Mathies RA, Glazer AN.
Journal: Anal Biochem (1998): 32