6-ROX glycine 25 uM fluorescence reference solution for PCR reactions

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Physical properties

Molecular weight	591.65
Solvent	Water

Spectral properties

Correction Factor (280 nm)	0.168
Extinction coefficient (cm ^-1 M ^-1)	82000
Excitation (nm)	578
Emission (nm)	604
Quantum yield	0.94¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

591.65

Correction Factor (280 nm)

0.168

Extinction coefficient (cm ^-1 M ^-1)

82000

Excitation (nm)

578

Emission (nm)

604

Quantum yield

0.94¹

6-ROX is predominately used as a reference dye for performing PCR detections. However, 6-ROX is very unstable compared to other rhodamine dyes. 6-ROX glycine has improved stability. The 6-ROX glycine has the similar spectral properties to those of 6-ROX. This 6-ROX dye is 25 uM solution in 20 mM Tris (pH 8.4), 0.1 mM EDTA and 0.01% Tween® 20.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of 6-ROX glycine *25 uM fluorescence reference solution for PCR reactions* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	169.019 µL	845.094 µL	1.69 mL	8.451 mL	16.902 mL
5 mM	33.804 µL	169.019 µL	338.038 µL	1.69 mL	3.38 mL
10 mM	16.902 µL	84.509 µL	169.019 µL	845.094 µL	1.69 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (280 nm)	0.168
Extinction coefficient (cm ^-1 M ^-1)	82000
Excitation (nm)	578
Emission (nm)	604
Quantum yield	0.94¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (280 nm)
5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] CAS 198978-94-8	578	604	82000	0.94¹	0.168
6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] CAS#: 216699-36-4	578	604	82000	0.94¹	0.168
5-ROX glycine Fluorescence reference standard for PCR reactions	578	604	82000	0.94¹	0.168
6-ROX azide	578	604	82000	0.94¹	0.168
6-ROX alkyne	578	604	82000	0.94¹	0.168

Images

Figure 1. Chemical structure for 6-ROX glycine *25 uM fluorescence reference solution for PCR reactions*

References

View all 11 references: Citation Explorer

Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926

Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence
Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834

Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488

Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412

Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
Authors: Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: Invest Ophthalmol Vis Sci (2003): 1312

HLA-DRB fluorotyping by dark quenching and automated analysis
Authors: Slateva K, Elsner HA, Albis-Camps M, Blasczyk R.
Journal: Tissue Antigens (2001): 250

Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction--amplified short tandem repeats during denaturing capillary electrophoresis
Authors: Hahn M, Wilhelm J, Pingoud A.
Journal: Electrophoresis (2001): 2691

Design, synthesis, and spectroscopic properties of peptide-bridged fluorescence energy-transfer cassettes
Authors: Li Y, Glazer AN.
Journal: Bioconjug Chem (1999): 241

Differential display with carboxy-X-rhodamine-labeled primers and the selection of differentially amplified cDNA fragments without cloning
Authors: Yoshikawa Y, Mukai H, Asada K, Hino F, Kato I.
Journal: Anal Biochem (1998): 82

Comparison of fluorescence energy transfer primers with different donor-acceptor dye combinations
Authors: Hung SC, Mathies RA, Glazer AN.
Journal: Anal Biochem (1998): 32